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The map label for this gene is gspE [H]

Identifier: 237814400

GI number: 237814400

Start: 4022518

End: 4024011

Strand: Direct

Name: gspE [H]

Synonym: GBP346_A4198

Alternate gene names: 237814400

Gene position: 4022518-4024011 (Clockwise)

Preceding gene: 237814399

Following gene: 237814401

Centisome position: 98.14

GC content: 69.61

Gene sequence:

>1494_bases
GTGACGCAGGCGCTCGCGCAAGGCGCCCCGGATCAGCCGGCCGCCGGCGCGCCGTCGCCGATCGCCGCGCGGCTGTTGCC
GTACGGCTTCGCGAAGGCCGGCCAGATCCTGATCGCACACCAGCACGCGGACACGCTCGAGGTGTGGATCAGCGAGCGCA
CGAGCTCGGCCGCGCTCGCCGAAGTCGCGCGCAACTTCGGCGCGATCTCGTTGCAGCGCATACCGGCCGACGAGCTCGCG
CAGGCGATCAACCATGCGTACGCGCGCCAGGACGGCAGCGCCGCGCAGATCGTCGGCGAGGTCGAGGGCGAAGTCGATCT
GTCGCGGCTGATGCAGGACATCCCCGAGGTCGAGGATCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGGATGATCA
ACGCGCTCCTCACGCAAGCGGCGCGCGAGCAGGCGTCGGACATCCACATCGAGCCGTTCGAGAACGCGTCGGTCGTGCGC
TTTCGCGTGGACGGCACGCTGCGCGACGTCGTGCGCCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGCATCAAGAT
CATGGCGCAGCTCGACATCGCCGAAAAGCGCCTGCCGCAGGACGGCCGGATCACGCTGCGCGTGGGCGGGCGGCCCGTCG
ACGTGCGGGTGTCGACGCTGCCGACCGGGCACGGCGAGCGCGCGGTGCTGCGTCTGCTGGAGAAGGATGCGCAGCGGCTG
AATCTGGAAGCGCTCGGGATGGGCCGCGACACGCTCGTGCAGTTCGACAAGCTGATTTCCCGCCCGCACGGCATCGTGCT
CGTGACGGGGCCGACCGGCTCGGGCAAGACGACGACGCTGTACGCGTCGATGTCGCGGCTCGAAACCGCGACGACCAACA
TCATGACGGTCGAGGATCCGATCGAATACGATCTGTCCGGCATCGGCCAGACGCAGGTGAACGAGCGGATCGGGATGACG
TTCGCGCGCGCGCTGCGCTCGATCCTGCGTCAGGACCCGGACATCATCATGATCGGCGAAATCCGCGATCTCGAAACCGC
GCAGATCGCCGTGCAGGCGTCGCTCACGGGCCACCTGGTGCTCGCGACGCTGCACACGAACGACGCGGCGTCGGCCGTCA
CGCGTCTCACCGACATGGGCGTCGAGCCGTACCTGCTTGCGTCTTCGCTGCTCGGCGTGCTCGCGCAGCGGCTCGTGCGC
CAGCTCTGCCCGGCGTGCAAGGAGGAGCGGCACGAGGGCGGCCGCGCGGTCTGGCATCCGGTCGGCTGCGACAAGTGCGG
GCATTCGGGCTACACGGGGCGTCGCGGCGTGTACGAGCTGCTCGTCATCGACGATTCGATCCGCTCGCTGATTCACCGCA
ACGCGGCCGATGCGGAGATTCTCGCCACCGGCCGCGCGAACGGGATGCGCACGCTGCGCGACGACGCCGAGCGCTGGCTC
GCGGCGGGCGCGACGTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCATAG

Upstream 100 bases:

>100_bases
ACCTCGACAAGATGCGGCGCCAGCAGCTCGCTCCGCCCGCGCCCGCCAGCGCGCCGGCGGCGGCGAGCGACGCCGGCACC
GCGTCGCCGGGAGCCCGTCC

Downstream 100 bases:

>100_bases
CGTCATGCCGGCCTTCCGTTTCGAAGCGATCGACGCGTCGGGGCGCGCGCAAAAAGGCGTGATCGAAGCCGACAGCGCGC
GCAACGCGCGCGGCCAGCTG

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTQALAQGAPDQPAAGAPSPIAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLQRIPADELA
QAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVR
FRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMT
FARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVR
QLCPACKEERHEGGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
AAGATSLEEVLRVTGGA

Sequences:

>Translated_497_residues
MTQALAQGAPDQPAAGAPSPIAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLQRIPADELA
QAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVR
FRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMT
FARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVR
QLCPACKEERHEGGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
AAGATSLEEVLRVTGGA
>Mature_496_residues
TQALAQGAPDQPAAGAPSPIAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALAEVARNFGAISLQRIPADELAQ
AINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRF
RVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRLN
LEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLSGIGQTQVNERIGMTF
ARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQ
LCPACKEERHEGGRAVWHPVGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWLA
AGATSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=53.8095238095238, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1786296, Length=434, Percent_Identity=43.5483870967742, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=35.8156028368794, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 53754; Mature: 53623

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQALAQGAPDQPAAGAPSPIAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALA
CCCCHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHCCCCHHHHH
EVARNFGAISLQRIPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDL
HHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCHHHHHH
LESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHC
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDP
CHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECCC
IEYDLSGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEE
LATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPACKEERHEGGRAVWHP
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
VGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAGATSLEEVLRVTGGA
HHCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQALAQGAPDQPAAGAPSPIAARLLPYGFAKAGQILIAHQHADTLEVWISERTSSAALA
CCCHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEHHHHHCCCCHHHHH
EVARNFGAISLQRIPADELAQAINHAYARQDGSAAQIVGEVEGEVDLSRLMQDIPEVEDL
HHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCHHHHHH
LESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALHGA
HCCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAQRL
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHHHC
NLEALGMGRDTLVQFDKLISRPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDP
CHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEECCC
IEYDLSGIGQTQVNERIGMTFARALRSILRQDPDIIMIGEIRDLETAQIAVQASLTGHLV
CHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCEEE
LATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPACKEERHEGGRAVWHP
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECC
VGCDKCGHSGYTGRRGVYELLVIDDSIRSLIHRNAADAEILATGRANGMRTLRDDAERWL
CCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
AAGATSLEEVLRVTGGA
HHCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]