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The map label for this gene is gcvH [H]

Identifier: 237814301

GI number: 237814301

Start: 3922763

End: 3923143

Strand: Direct

Name: gcvH [H]

Synonym: GBP346_A4099

Alternate gene names: 237814301

Gene position: 3922763-3923143 (Clockwise)

Preceding gene: 237814300

Following gene: 237814302

Centisome position: 95.71

GC content: 64.83

Gene sequence:

>381_bases
ATGAGCAACGTCCCGGCCGAACTGAAATACACCGACGAACACGAGTGGATTCGCACCGAAGCCGACGGCACGCTGACGAT
CGGCATCACCGACCACGCGCAAACCACGCTCGGCGACATCGTCTTCCTCGAGCTGCCGAAAGTCGGCTCGCAAGTGAAGG
CGGGCGACGCCGTCGGCGTCGTCGAATCGGTGAAAGCCGCGTCGGACATCTACTCGCCCGTGACCGGCGAAGTGATCGCG
ATCAACACGGACGTGGCCGATGCGCCGGAAGAGGTGAACGGCGACGCGTACGGCGCATGGCTCTTCAAGATCAAGCTGGC
GGCCGACGCCTCGACCGACAGCCTGCTCGACGCCGCCGCCTACGAGAAGCTGGTCGGCTGA

Upstream 100 bases:

>100_bases
TGCGTAACGGGCCGGGCGGCGCGCCGCCCGGCGATACCGTACCCGGCGCGCCGATTGCGCGCCAGAAAGAATCACACCCT
TTTCCATTCAGGAGCATTCG

Downstream 100 bases:

>100_bases
CCCGCACCTTACTCATCGCGCGGCGCGCGGCCCGGCGGCATGGGTCGTGCACCGCGGGGGACCGGGGGAGGTGCTGAAAC
GCCGGCCCTGGTCGATTGCG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSNVPAELKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPKVGSQVKAGDAVGVVESVKAASDIYSPVTGEVIA
INTDVADAPEEVNGDAYGAWLFKIKLAADASTDSLLDAAAYEKLVG

Sequences:

>Translated_126_residues
MSNVPAELKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPKVGSQVKAGDAVGVVESVKAASDIYSPVTGEVIA
INTDVADAPEEVNGDAYGAWLFKIKLAADASTDSLLDAAAYEKLVG
>Mature_125_residues
SNVPAELKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPKVGSQVKAGDAVGVVESVKAASDIYSPVTGEVIAI
NTDVADAPEEVNGDAYGAWLFKIKLAADASTDSLLDAAAYEKLVG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=118, Percent_Identity=44.9152542372881, Blast_Score=110, Evalue=4e-25,
Organism=Homo sapiens, GI89057342, Length=118, Percent_Identity=44.0677966101695, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=61.9047619047619, Blast_Score=166, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=42.2413793103448, Blast_Score=101, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=42.2413793103448, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=41.2698412698413, Blast_Score=102, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17865652, Length=120, Percent_Identity=40.8333333333333, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13252; Mature: 13121

Theoretical pI: Translated: 3.94; Mature: 3.94

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPAELKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPKVGSQVKAGDAVGV
CCCCCCCEEECCCCCEEEECCCCEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCHHHH
VESVKAASDIYSPVTGEVIAINTDVADAPEEVNGDAYGAWLFKIKLAADASTDSLLDAAA
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHH
YEKLVG
HHHHCC
>Mature Secondary Structure 
SNVPAELKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPKVGSQVKAGDAVGV
CCCCCCEEECCCCCEEEECCCCEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCHHHH
VESVKAASDIYSPVTGEVIAINTDVADAPEEVNGDAYGAWLFKIKLAADASTDSLLDAAA
HHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHH
YEKLVG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA