Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 237814231

GI number: 237814231

Start: 3856788

End: 3857522

Strand: Reverse

Name: fliA [H]

Synonym: GBP346_A4026

Alternate gene names: 237814231

Gene position: 3857522-3856788 (Counterclockwise)

Preceding gene: 237814232

Following gene: 237814229

Centisome position: 94.12

GC content: 66.53

Gene sequence:

>735_bases
ATGATGTATAACGCTCAAGGCAAGATTTCCCAGGACGAAGTGCTGACGCAATACGCGCCGCTCGTGCGTCGTCTCGGCCT
GCAGCTCGTCGCGAAGATGCCGGCGAGCGTGGACCTCGACGATCTGATCCAGGCGGGCATGATCGGCCTGCTCGACGCGG
CGAGCCGCTACAAGGAAGATCAGGGCGCGCAGTTCGAGACCTACGCGACGCAGCGCATCCGCGGCGCGATGCTCGACGAG
CTGCGCAGCAACGATTGGCTGCCGCGCAGCCTGCGCAAGACCTCGCGCGAAGTCGAACATGCGGTGCATCAGGTCGAGCA
GCGGCTCGGCCGCTCGGCGAACGAGACGGAGATCGCCGCGCACCTGCAGATGCCGCTCGACGAATACCAGTCGATGCTGC
AGGATCTGCACGGCAGCCAGCTCGTCTACTACGAGGACTTCGACCGCTCGGCCGACGACGAGCCGTTTCTCGACCGCTAC
CGCGCCGATCACAGCGATCCGCTGTCGGCGCTGCTCGACGAGCATCTGCGCAGCGCGCTCGTCGATGCGATCGAGCGCCT
GCCGGAGCGCGAGAAGCTGCTGATGTCGCTGTACTACGAGCGCGGGCTGAACCTGCGCGAGATCGGTGCGGTGCTCGAGG
TGAGCGAGTCGCGCGTGTGCCAGTTGCATAGCCAGGCGGTCGCGCGGCTACGCGCGAAGCTGCGCGAGCAGGCGTGGGTC
GGCACGGAGAGCTGA

Upstream 100 bases:

>100_bases
GATGCGCCCGGGTCCGGGCATCGGCCGGGTCCGGACGGGAAGTTCGGCGTTTGAACGCGGCGCGGCTCATGCCGCCTGAA
CGCCACGACGAAGAGAGGGC

Downstream 100 bases:

>100_bases
CGCGGCCGCGCGGCGCCCGGGCGAGCGAAGCGCGGCTTGAAATGCGGGCCCGAAGGCGCGCCGGCGACGCGGGCGGAATG
CCGTCGGAGCGCGGCACGGA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MMYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDE
LRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRY
RADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWV
GTES

Sequences:

>Translated_244_residues
MMYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDE
LRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRY
RADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWV
GTES
>Mature_244_residues
MMYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKEDQGAQFETYATQRIRGAMLDE
LRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAAHLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRY
RADHSDPLSALLDEHLRSALVDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWV
GTES

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=233, Percent_Identity=53.6480686695279, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1789448, Length=205, Percent_Identity=28.2926829268293, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27928; Mature: 27928

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
HLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYRADHSDPLSALLDEHLRSAL
HHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
VDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWV
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTES
CCCC
>Mature Secondary Structure
MMYNAQGKISQDEVLTQYAPLVRRLGLQLVAKMPASVDLDDLIQAGMIGLLDAASRYKED
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGAQFETYATQRIRGAMLDELRSNDWLPRSLRKTSREVEHAVHQVEQRLGRSANETEIAA
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
HLQMPLDEYQSMLQDLHGSQLVYYEDFDRSADDEPFLDRYRADHSDPLSALLDEHLRSAL
HHHCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
VDAIERLPEREKLLMSLYYERGLNLREIGAVLEVSESRVCQLHSQAVARLRAKLREQAWV
HHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]