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The map label for this gene is hisH [H]

Identifier: 237814062

GI number: 237814062

Start: 3699429

End: 3700070

Strand: Reverse

Name: hisH [H]

Synonym: GBP346_A3847

Alternate gene names: 237814062

Gene position: 3700070-3699429 (Counterclockwise)

Preceding gene: 237814063

Following gene: 237814061

Centisome position: 90.28

GC content: 66.67

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCAATTGTGGATTACGGAATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCATGAAGGCGGAGCC
GGCGGCCGACGTGAAGATCGTCGACCGGCCCGAGGCGATTCGCGCGGCCGACCGCGTGGTGCTGCCGGGCCAGGGCGCGA
TGCCCGACTGCATGCGGTACCTCGGCGAATCCGGCTTGCAGGAGGCGGTGGTCGAGGCGTCGCGCACGAAGCCGCTGCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAAGGGGCTCGGCCTGCTGCCGGGCAA
GGTGGTGCGCTTCGCGCTCGAGGGCCGGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGGG
TGCGCCAGACCCAGGCGCATCCGTTGTGGGACGGCGTGCCGGATGACGCCTATTTCTACTTCGTGCACAGTTATTACGTG
GTGCCGGACAACCCGGCGCACACGGCGGGCGAGACCCTGTACGGCGACGTTTTCACGTCCGCGGTCGCGCGCGACAATCT
CTTCGCGACCCAATTTCACCCCGAAAAGAGCGCCGAAGTGGGGCTGCGGCTGTACCGGAATTTTGTCCACTGGAACCCCT
GA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGTCACGCGTCTGATGGGATTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCGACGGACTGAAG
GCATTATTGCCTGCACTGAC

Downstream 100 bases:

>100_bases
ACGTGGCGCGGGAACCACACTCGGCGTGCGCAAAACGCCCGCCGCACGGGCTTCGCGAGCGGATACGCAGAAAGAGTTGT
ACTAAACTAGCGAGACGGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRYLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRYLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRYLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.0576923076923, Blast_Score=121, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=31.651376146789, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23573; Mature: 23573

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRY
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETLYGDVFTS
CCEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRY
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETLYGDVFTS
CCEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]