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The map label for this gene is nuoB2 [H]

Identifier: 237813963

GI number: 237813963

Start: 3615899

End: 3616402

Strand: Direct

Name: nuoB2 [H]

Synonym: GBP346_A3747

Alternate gene names: 237813963

Gene position: 3615899-3616402 (Clockwise)

Preceding gene: 237813961

Following gene: 237813964

Centisome position: 88.22

GC content: 65.67

Gene sequence:

>504_bases
ATGGCGAATCATCCTCTCACCCTCGAAAAAGACGGCTTCATCGTCACGACGCTCGACGCCGCGATGGCCGCCGCGCAGAA
AAACAGCCTCTGGTACATGACGTTCGGCCTCGCGTGTTGCGCGGTCGAGATGATGCACGCGGCGGGCGCGCGTTACGACA
TGGACCGTTTCGGCATGATCCCGCGCGCGTCGCCGCGCCAGTGCGATCTGATGATCGTCGCCGGCACGCTGACCAACAAG
ATGGCGCCCGCGATGCGCCGCGTCTACGACCAGATGGCCGAACCGCGCTACGTCGTGTCGATGGGCTCGTGCGCGAACGG
CGGCGGCTATTACCACTACGGCTATTCGGTCGTGCGCGGCTGCGATCGCATCGTGCCCGTCGACGTCTACGTACCCGGCT
GCCCGCCGACCGCCGAGGCGCTCGTCTACGGTCTGATGCAACTGCAGCGCAAGGTCGCCGAGCGCAGCACGCATAGTCGG
CCTAAACTGTTCGCGCGACCCTGA

Upstream 100 bases:

>100_bases
CGCGCGCCCTCGCCTCGCCTCGCGTCGCATTCGTACAAGCCATTCGGCACGGCCGCGTATAGAGTCTGCGCTCGTCACCG
GCTCTTCGACTCCATTCGGC

Downstream 100 bases:

>100_bases
CCGCCGACAATCCATGCCCGCCTATCGACGACTCCACGCGCCTCACCCGCGCCTGCGCGACATCGTGAACTGCTATTTCG
TCGAGGTCGGGCGCGCGGGC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASPRQCDLMIVAGTLTNK
MAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYGYSVVRGCDRIVPVDVYVPGCPPTAEALVYGLMQLQRKVAERSTHSR
PKLFARP

Sequences:

>Translated_167_residues
MANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASPRQCDLMIVAGTLTNK
MAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYGYSVVRGCDRIVPVDVYVPGCPPTAEALVYGLMQLQRKVAERSTHSR
PKLFARP
>Mature_166_residues
ANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMIPRASPRQCDLMIVAGTLTNKM
APAMRRVYDQMAEPRYVVSMGSCANGGGYYHYGYSVVRGCDRIVPVDVYVPGCPPTAEALVYGLMQLQRKVAERSTHSRP
KLFARP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=148, Percent_Identity=74.3243243243243, Blast_Score=241, Evalue=3e-64,
Organism=Escherichia coli, GI1788624, Length=157, Percent_Identity=49.0445859872611, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI1789074, Length=159, Percent_Identity=32.0754716981132, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=38.8429752066116, Blast_Score=94, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=66.6666666666667, Blast_Score=227, Evalue=3e-60,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=73.5714285714286, Blast_Score=232, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=73.5714285714286, Blast_Score=232, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24651058, Length=140, Percent_Identity=74.2857142857143, Blast_Score=229, Evalue=7e-61,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18439; Mature: 18308

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
7.8 %Met     (Translated Protein)
11.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
7.2 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMI
CCCCCCEEECCCEEEEEHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCC
PRASPRQCDLMIVAGTLTNKMAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYGYSVVRG
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHC
CDRIVPVDVYVPGCPPTAEALVYGLMQLQRKVAERSTHSRPKLFARP
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ANHPLTLEKDGFIVTTLDAAMAAAQKNSLWYMTFGLACCAVEMMHAAGARYDMDRFGMI
CCCCCEEECCCEEEEEHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCC
PRASPRQCDLMIVAGTLTNKMAPAMRRVYDQMAEPRYVVSMGSCANGGGYYHYGYSVVRG
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHHHHHC
CDRIVPVDVYVPGCPPTAEALVYGLMQLQRKVAERSTHSRPKLFARP
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA