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The map label for this gene is pcaF_1 [H]

Identifier: 237813938

GI number: 237813938

Start: 3594145

End: 3595362

Strand: Direct

Name: pcaF_1 [H]

Synonym: GBP346_A3721

Alternate gene names: 237813938

Gene position: 3594145-3595362 (Clockwise)

Preceding gene: 237813937

Following gene: 237813939

Centisome position: 87.69

GC content: 72.25

Gene sequence:

>1218_bases
TTGCCGGAATTCGACATGACCGAAGCATTCCTGTGTGACGCAATCCGCACGCCCATCGGCCGCTACGGCGGCGCGCTGGC
GCCGGTGCGGGCCGACGACCTCGGCGCGGTGCCGCTCAAGGCGCTCGTCGAGCGCAACCGCGACGTCGACTGGGCCGCCG
TCGACGACGTGATCTACGGCTGCGCGAACCAGGCCGGCGAGGACAACCGCAACGTCGCGCGCATGTCGCTGCTGCTCGCC
GGCTTGCCGCACGCCGTGCCCGGCGCGACGATCAACCGCCTCTGCGGCTCCGGGATGGACGCGATCGGCCTCGCCGCGCG
CGCGATCAAGGCGGGTGAGGCGAGCCTGATGATCGCCGGCGGCGTCGAGAGCATGAGCCGCGCGCCGTTCGTGACCGGCA
AGGCGACGAGCGCGTTCTCGCGCCAGGCCGAGATCTTCGATACGACCATCGGCTGGCGCTTCGTCAATCCGCTGATGAAA
CAGCGGTACGGCGTCGATTCGATGCCGGAGACGGCCGAAAATGTCGCGACCGATTACCGCGTGAGCCGCGCCGATCAGGA
CGCGTTCGCGCTGCGCAGCCAGCAGAAAGCGGCGCGCGCGCAGGCCGACGGCACGCTCGCGCAGGAGATCGCGCCGGTGA
CGATCGCGCAGAAGAAGGGCGAGCCGCTCGTCGTGGCGCGCGACGAGCATCCGCGCGAGACGACCCTCGAGGCGCTCGCG
AAACTCAAGGGCGTGGTGCGCCCGGACGGCACGGTGACGGCGGGCAACGCGTCGGGCGTGAACGACGGTGCGTGCGCGCT
GCTGGTGGCGAGCGAGGCGGCGGCGCGCCGCCACGGGCTCGTGCCGCGCGCGCGCGTGCTGGGGATCGCGACGGCGGGCG
TGGAGCCGCGGGTGATGGGGATCGGCCCGGCGCCGGCGACGCAGCAACTGCTCGCGCGGCTGGGGATGACGCTCGACCAG
TTCGACGTGATCGAGCTGAACGAGGCGTTCGCGTCGCAGGGGCTCGCGGTGCTGCGGCTGCTGGGCGTCGCGGACGACGA
TGCGCGGGTGAATCCGAACGGCGGCGCGATCGCGCTCGGGCATCCGCTTGGCGCGTCGGGCGCGCGGCTCGTGACGACCG
CCACGTATCAACTGCACCGCACGGGCGGCCGCTTCGCGCTCTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGATC
GCGATCGAGCGCGTGTGA

Upstream 100 bases:

>100_bases
CCCGCGGAAGCCGGCCAAACGGCATAACCCGTTCGGCCGAGCTGTCGATCGCGCGACCGTTCACTACCATGAATGAACCC
GCCCGCTCGCGGGTTCACCT

Downstream 100 bases:

>100_bases
TTGGCGCCGCCGCGCGACAGCGCGGCGGATGTCTACAAGCGGCCCGCGGACGAAGGACGCATCCCGTTCATTTCGAAGAG
GCAAGGAGACAGCCGATGTC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLA
GLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMK
QRYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKKGEPLVVARDEHPRETTLEALA
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQQLLARLGMTLDQ
FDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAI
AIERV

Sequences:

>Translated_405_residues
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLA
GLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMK
QRYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKKGEPLVVARDEHPRETTLEALA
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQQLLARLGMTLDQ
FDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAI
AIERV
>Mature_404_residues
PEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYGCANQAGEDNRNVARMSLLLAG
LPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAGGVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQ
RYGVDSMPETAENVATDYRVSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKKGEPLVVARDEHPRETTLEALAK
LKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMGIGPAPATQQLLARLGMTLDQF
DVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALGHPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIA
IERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=42.5316455696203, Blast_Score=284, Evalue=8e-77,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=39.5465994962217, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=34.2298288508557, Blast_Score=225, Evalue=7e-59,
Organism=Homo sapiens, GI4501853, Length=410, Percent_Identity=38.0487804878049, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.1475409836066, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI194353979, Length=408, Percent_Identity=28.4313725490196, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=68.5785536159601, Blast_Score=559, Evalue=1e-160,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=45.8852867830424, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.9057071960298, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.2098765432099, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=31.6301703163017, Blast_Score=165, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=43.7185929648241, Blast_Score=300, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=34.1584158415842, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=33.9901477832512, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=31.7865429234339, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=29.3532338308458, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=407, Percent_Identity=35.8722358722359, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=403, Percent_Identity=34.4913151364764, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.3915211970075, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=39.1625615763547, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=35.5555555555556, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=30.6603773584906, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24585051, Length=320, Percent_Identity=26.5625, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921506, Length=320, Percent_Identity=25.9375, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42312; Mature: 42181

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYG
CCCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CANQAGEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYR
CHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKKGEPLVVARDEHPRETTLEALA
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHH
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMG
HHHCCCCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEE
IGPAPATQQLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALG
CCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEEC
HPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
PEFDMTEAFLCDAIRTPIGRYGGALAPVRADDLGAVPLKALVERNRDVDWAAVDDVIYG
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
CANQAGEDNRNVARMSLLLAGLPHAVPGATINRLCGSGMDAIGLAARAIKAGEASLMIAG
HHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEC
GVESMSRAPFVTGKATSAFSRQAEIFDTTIGWRFVNPLMKQRYGVDSMPETAENVATDYR
CHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VSRADQDAFALRSQQKAARAQADGTLAQEIAPVTIAQKKGEPLVVARDEHPRETTLEALA
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCEEEECCCCCEEEEECCCCCHHHHHHHHH
KLKGVVRPDGTVTAGNASGVNDGACALLVASEAAARRHGLVPRARVLGIATAGVEPRVMG
HHHCCCCCCCCEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCHHEEEEEECCCCCEEEE
IGPAPATQQLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDARVNPNGGAIALG
CCCCCHHHHHHHHHCCCCCCCCEEEHHHHHHHCCHHHHHHHCCCCCCCEECCCCCEEEEC
HPLGASGARLVTTATYQLHRTGGRFALCTMCIGVGQGIAIAIERV
CCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11741863 [H]