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The map label for this gene is pilD [H]

Identifier: 237813902

GI number: 237813902

Start: 3549389

End: 3550303

Strand: Direct

Name: pilD [H]

Synonym: GBP346_A3681

Alternate gene names: 237813902

Gene position: 3549389-3550303 (Clockwise)

Preceding gene: 237813901

Following gene: 237813903

Centisome position: 86.6

GC content: 70.49

Gene sequence:

>915_bases
ATGACGCCCAATCCGTTCCTTTCCGGCTCGCCCGAACATGCGGCGGCGGCCGGGCCGCTCGCCGCATTCGCGGCGCTCCC
CACGGGCATGCAACTTGCGTTCGCGATCGTGCTCGGCCTCGTCGTCGGCAGCTTCATCAACGTCGTCGTCCATCGGCTGC
CGATCATGATGAAGCGCGCGTGGCTCGCCGAAATCGCCGAAGCGACGGGCGCGCCGTGCGCCGACGACGGCCTGCCGGCG
CGCTACAACCTCTGCGTGCCGCGCAGCGCGTGCCCGCACTGCGGCCATGCGCTGCGCGCGTGGGAAAACGTGCCGGTCCT
CAGCTATATCGCATTGCGGGGCCGGTGCCGGCACTGCCGTACGCCGATCGGCGCGCGCTATCCGCTCATCGAACTCGCGA
GCGGCGCGCTCGCCGCCGGCGCGCTCGCGCTGTTCGGGCCGAGCGGCGCCGCACTCGCCGCGTTCGGCCTGTGCGCGGCG
CTCCTCGCGATGAGCGCGATCGACATGCAGACCGGCTTCCTGCCCGATTCGCTCACGTTGCCGCTCCTGTGGGCCGGGCT
GTGCGTCAATCTCTGGGGCACGTTTGCGAGCCTGCGCGCCGCGGTGATCGGCGCGATCGCCGGCTATCTGTTCCTCTGGT
GCATTCTGTGGCTCTTCAAACTGCTGCGCGGCATCGAAGGAATCGGCTACGGCGACCTGAAGCTCCTCGCCGCGCTCGGC
GCATGGCTCGGCTGGGAAGCATTGCCGCAGGTCGTGCTGATCGCAGCGGTCGCCGGCGCCGCGGTCGGTCTCGTCGCAAC
CTGGCGCGGCCGCATGCGCTTCGAGGAACCGTTGCCATTCGGCCCGTTCCTCGCGGCGGGCGGCGCCGCGACCCTCTTTT
TCGGCACGCCGTTCTACCTGCTGCTCGGAGGCTGA

Upstream 100 bases:

>100_bases
GCGCGCTCGTCGTCGCGATGTACTTGCCGATCGTCCAGCTCGGCAACGTGGTGTAGCATCGCAGCCGAATCCGACTCCAT
TTGCGATGCCAACCGCCTCG

Downstream 100 bases:

>100_bases
ACCATGTTCTCGGTGGGATTGACGGGCGGCATCGGTAGCGGCAAGACGACCGTGGCCGATTTGTTCGGTAAGCTCGGCGC
GACGATCGTCGATACCGATC

Product: type IV prepilin leader peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTPNPFLSGSPEHAAAAGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVVHRLPIMMKRAWLAEIAEATGAPCADDGLPA
RYNLCVPRSACPHCGHALRAWENVPVLSYIALRGRCRHCRTPIGARYPLIELASGALAAGALALFGPSGAALAAFGLCAA
LLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWGTFASLRAAVIGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALG
AWLGWEALPQVVLIAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYLLLGG

Sequences:

>Translated_304_residues
MTPNPFLSGSPEHAAAAGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVVHRLPIMMKRAWLAEIAEATGAPCADDGLPA
RYNLCVPRSACPHCGHALRAWENVPVLSYIALRGRCRHCRTPIGARYPLIELASGALAAGALALFGPSGAALAAFGLCAA
LLAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWGTFASLRAAVIGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALG
AWLGWEALPQVVLIAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYLLLGG
>Mature_303_residues
TPNPFLSGSPEHAAAAGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVVHRLPIMMKRAWLAEIAEATGAPCADDGLPAR
YNLCVPRSACPHCGHALRAWENVPVLSYIALRGRCRHCRTPIGARYPLIELASGALAAGALALFGPSGAALAAFGLCAAL
LAMSAIDMQTGFLPDSLTLPLLWAGLCVNLWGTFASLRAAVIGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALGA
WLGWEALPQVVLIAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYLLLGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=278, Percent_Identity=37.410071942446, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=47.7272727272727, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31707; Mature: 31576

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPNPFLSGSPEHAAAAGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVVHRLPIMMKRA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLAEIAEATGAPCADDGLPARYNLCVPRSACPHCGHALRAWENVPVLSYIALRGRCRHCR
HHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHC
TPIGARYPLIELASGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTL
CCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
PLLWAGLCVNLWGTFASLRAAVIGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AWLGWEALPQVVLIAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEE
LLGG
EECC
>Mature Secondary Structure 
TPNPFLSGSPEHAAAAGPLAAFAALPTGMQLAFAIVLGLVVGSFINVVVHRLPIMMKRA
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLAEIAEATGAPCADDGLPARYNLCVPRSACPHCGHALRAWENVPVLSYIALRGRCRHCR
HHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHC
TPIGARYPLIELASGALAAGALALFGPSGAALAAFGLCAALLAMSAIDMQTGFLPDSLTL
CCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
PLLWAGLCVNLWGTFASLRAAVIGAIAGYLFLWCILWLFKLLRGIEGIGYGDLKLLAALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AWLGWEALPQVVLIAAVAGAAVGLVATWRGRMRFEEPLPFGPFLAAGGAATLFFGTPFYL
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCEEE
LLGG
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]