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The map label for this gene is citH [H]

Identifier: 237813731

GI number: 237813731

Start: 3397003

End: 3398583

Strand: Reverse

Name: citH [H]

Synonym: GBP346_A3508

Alternate gene names: 237813731

Gene position: 3398583-3397003 (Counterclockwise)

Preceding gene: 237813732

Following gene: 237813724

Centisome position: 82.92

GC content: 70.21

Gene sequence:

>1581_bases
GTGCGATGCGCGGCCGCCGCGTCGCGCCGACGAACCGCCGCGTTCCATATCGGGCGCGGCGGTTTTCTTTTTTTGGCGTG
CGTGGCGCATGCCTATAATCGTGCGCGTTCGTCTTCGTCCGCCTTCGCCGTTCGAGGCGGCGCGGCGATCGGCGCGCGCA
TCGTGGCGGGCGGGACGGTTCGTGACGCACGCTGCGCGCGTGCGCGCCCGCGACGACGGAGCGCTCGCGCCGACGGCAGC
GGCACGGCAGCGACGGACAACAACGACGGACGACAGCGACCACGCTCAGGAGACGCCATGCATGCAGCCGACGCGGCTTC
GCCCGCGACCGATACCGTCCCCCCGCGCGTGTGGCGCGCCGTCGTCGCCGCGTCGATCGGCAACGCGCTCGAATGGTTCG
ATCTCGTCGTCTACGGTTTCTTCGCGGTCACGATCTCGCGGCTGTTCTTTCCCGCCGGCAACGACGCGGTGTCGCTGCTG
CTGACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGGCCGCTCGGCGCGATCGTGCTCGGCGCATACGCGGACCGCCA
CGGCCGCAAGGCCGCGCTCACGCTGTCGATCCTGCTGATGATGGCGGGCACGTTCGTCATCGCGGTGCTGCCGACCTACG
CGACGATCGGGATCGCGGCGCCCGTCGTGCTCGTCGGCGCGCGGCTGATGCAGGGCTTCTCCGCGGGCGGCGAATTCGGC
AGCGCGACCGCGTTTCTCGCCGAGCACGTGCCCGGGCGGCGCGGTTTCTTCGCGAGCTGGCAGGTCGCGAGCCAGGGGCT
CACGACGCTCCTCGCGGCGATCTTCGGCACGGCGCTCAACGCGGGGCTCACGAGCGCGCAGATGGCGGCATGGGGCTGGC
GCGTGCCGTTCTGCTTCGGGTTGCTGCTCGGGCCCGTCGCGTACTACATTCGTTCGAAAGTCGACGAGACGCCCGAATTC
CTCGCGTCGGCGCCCGCCGCGAACCCGCTGCGCGACACGTTCGCCGCGCACAAGGGGCGGCTTGCCGCGGCGATCGGCGC
GGTCGTGCTCGGCACCGTCGCGACTTACCTCGTGCTGTTCATGCCGACCTACGGCGTGAAGGAGCTGAAGCTCGCGCCGT
CCGCGGCGTTCGCCGCGATTCTCGTCGTCGGCGTGATCCAGATGGGTTTCGCGCCGGTGGTCGGGCACTGGTCCGATCGC
CACGGCCGCGTGCGGACGATGTGCGTGCCCGCGCTCGCGATCCTCGTGCTGATCTACCCGGCGTTCGCGTGGCTCGCCGC
GCATCCGACGTTCGGCGCGCTGATCGCCGTGCAGATCGTGTTCGCGTTCCTGATGACCGGCTATTTCGCCGCGCTGCCGG
GCCTGCTTTCGGAGATCTTTCCGGTTGCGACGCGCACGACCGGCATGTCGCTCGCGTATAACGTCGCGGTGACGGTCTTC
GGCGGATTCGGCCCGTTCATCATCGCGTGGCTGATCCGTGCGACGGGCACGAAAGCCGCGCCCAGCTTCTATCTGATCTT
CGCCGCGCTCGCGAGCCTCGCGGCGCTCGTCGCGCTGCGGCGGCGCTTCGGGTTGCGCTGA

Upstream 100 bases:

>100_bases
GACTACGGCAAGCAGTACGGCTTCAAGATGAAGACGAAGCTGCTCATCACGGCCGAAGCGGTCAAGCAGTAAGCGTTTTC
GCGAGGCCGCCGCGCGCCGC

Downstream 100 bases:

>100_bases
CGCGCGGCGTTCGGCGCGCCCCGCATCCGGCGGCCCAAGCCTCGTCGGCTCAAACGTCGCCGACTTGGGCCTCTGCGCCA
CGGCCGGCGGAGCCGGAACG

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MRCAAAASRRRTAAFHIGRGGFLFLACVAHAYNRARSSSSAFAVRGGAAIGARIVAGGTVRDARCARARPRRRSARADGS
GTAATDNNDGRQRPRSGDAMHAADAASPATDTVPPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLL
LTLGTFGVSFFMRPLGAIVLGAYADRHGRKAALTLSILLMMAGTFVIAVLPTYATIGIAAPVVLVGARLMQGFSAGGEFG
SATAFLAEHVPGRRGFFASWQVASQGLTTLLAAIFGTALNAGLTSAQMAAWGWRVPFCFGLLLGPVAYYIRSKVDETPEF
LASAPAANPLRDTFAAHKGRLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDR
HGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR

Sequences:

>Translated_526_residues
MRCAAAASRRRTAAFHIGRGGFLFLACVAHAYNRARSSSSAFAVRGGAAIGARIVAGGTVRDARCARARPRRRSARADGS
GTAATDNNDGRQRPRSGDAMHAADAASPATDTVPPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLL
LTLGTFGVSFFMRPLGAIVLGAYADRHGRKAALTLSILLMMAGTFVIAVLPTYATIGIAAPVVLVGARLMQGFSAGGEFG
SATAFLAEHVPGRRGFFASWQVASQGLTTLLAAIFGTALNAGLTSAQMAAWGWRVPFCFGLLLGPVAYYIRSKVDETPEF
LASAPAANPLRDTFAAHKGRLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDR
HGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR
>Mature_526_residues
MRCAAAASRRRTAAFHIGRGGFLFLACVAHAYNRARSSSSAFAVRGGAAIGARIVAGGTVRDARCARARPRRRSARADGS
GTAATDNNDGRQRPRSGDAMHAADAASPATDTVPPRVWRAVVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLL
LTLGTFGVSFFMRPLGAIVLGAYADRHGRKAALTLSILLMMAGTFVIAVLPTYATIGIAAPVVLVGARLMQGFSAGGEFG
SATAFLAEHVPGRRGFFASWQVASQGLTTLLAAIFGTALNAGLTSAQMAAWGWRVPFCFGLLLGPVAYYIRSKVDETPEF
LASAPAANPLRDTFAAHKGRLAAAIGAVVLGTVATYLVLFMPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDR
HGRVRTMCVPALAILVLIYPAFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=31.8604651162791, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788942, Length=441, Percent_Identity=34.0136054421769, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788292, Length=411, Percent_Identity=31.6301703163017, Blast_Score=168, Evalue=7e-43,
Organism=Escherichia coli, GI1789941, Length=428, Percent_Identity=33.8785046728972, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082231, Length=219, Percent_Identity=29.2237442922374, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 55228; Mature: 55228

Theoretical pI: Translated: 11.64; Mature: 11.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCAAAASRRRTAAFHIGRGGFLFLACVAHAYNRARSSSSAFAVRGGAAIGARIVAGGTV
CCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCEEEECCCC
RDARCARARPRRRSARADGSGTAATDNNDGRQRPRSGDAMHAADAASPATDTVPPRVWRA
CHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
VVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLLLTLGTFGVSFFMRPLGAIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAYADRHGRKAALTLSILLMMAGTFVIAVLPTYATIGIAAPVVLVGARLMQGFSAGGEFG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SATAFLAEHVPGRRGFFASWQVASQGLTTLLAAIFGTALNAGLTSAQMAAWGWRVPFCFG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
LLLGPVAYYIRSKVDETPEFLASAPAANPLRDTFAAHKGRLAAAIGAVVLGTVATYLVLF
HHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
MPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDRHGRVRTMCVPALAILVLIYP
HCCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEE
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRCAAAASRRRTAAFHIGRGGFLFLACVAHAYNRARSSSSAFAVRGGAAIGARIVAGGTV
CCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCEEEECCCC
RDARCARARPRRRSARADGSGTAATDNNDGRQRPRSGDAMHAADAASPATDTVPPRVWRA
CHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
VVAASIGNALEWFDLVVYGFFAVTISRLFFPAGNDAVSLLLTLGTFGVSFFMRPLGAIVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GAYADRHGRKAALTLSILLMMAGTFVIAVLPTYATIGIAAPVVLVGARLMQGFSAGGEFG
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SATAFLAEHVPGRRGFFASWQVASQGLTTLLAAIFGTALNAGLTSAQMAAWGWRVPFCFG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHH
LLLGPVAYYIRSKVDETPEFLASAPAANPLRDTFAAHKGRLAAAIGAVVLGTVATYLVLF
HHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
MPTYGVKELKLAPSAAFAAILVVGVIQMGFAPVVGHWSDRHGRVRTMCVPALAILVLIYP
HCCCCCHHHCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
AFAWLAAHPTFGALIAVQIVFAFLMTGYFAALPGLLSEIFPVATRTTGMSLAYNVAVTVF
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEE
GGFGPFIIAWLIRATGTKAAPSFYLIFAALASLAALVALRRRFGLR
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]