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The map label for this gene is phnD [H]

Identifier: 237813703

GI number: 237813703

Start: 3369515

End: 3370510

Strand: Reverse

Name: phnD [H]

Synonym: GBP346_A3480

Alternate gene names: 237813703

Gene position: 3370510-3369515 (Counterclockwise)

Preceding gene: 237813704

Following gene: 237813702

Centisome position: 82.24

GC content: 65.56

Gene sequence:

>996_bases
ATGAAGCTGATCCGCTCCCTCGTCGCCTGCGTGGCCGCGCTCGCGTGCGCCGCCGCCGCTCATGCGCAAACGCTGAACTT
CGGCATCATCTCCACCGATTCGTCGGCGGTGCTCAAGCGCCGCTGGCAACCGCTCATCGACGACATGAACCGCAAGACGG
GCCTCGACGTGAAGGCGTTCTTCGCGACCGACTACGCGGGCATCATCGAGGGCATGCGCTTCAACAAGGTACAGCTCGGC
TACTTCGGCAACGCGTCGGCGCTCGAGGCGGTCGAGCGCTCGAACGGCGAGGTGTTCGCGAAGCTTCGCCACGCGAACGG
CGAGACCGGCTACTACTCGGTGCTGATCACGAACGCGAACAGCCGCTTCAGGACGCTTGACGACGTGTTCAGGAACCCGA
AGGACGTGACGCTCGGCTTCGGCGATCCGAACTCGACGTCCGGCACGCTCGTGCCCGCGTACTATCTGTTCGCGCCGCGC
GGCATAGCGCCGCGCACCGCGTTCAGGACGGTGCTGCCGTCGAGCCACGAGGGCAATCTGCTCGCGGTCGTCAACGACAA
GATCGACATCGCGACGAACAACACCGACGTGCTCGGCATGCTGAAGAAGCAGCATCCGGATCAATACGCGCGGGTGCGCG
TGCTGTGGACATCGCCGCAGATCGCATCCGATCCGCTCGTGTGGCGCAAGGATCTGCCCGACGCGACCAAGCAGAAGCTG
CGCGATTTCTTCTACAACTACGCGAAGACCGATCCGCACGAGCAGGCGGTAATGGCGGGCATTCGCAATCTGGTCGGCTT
CGCGCCGTCGTCGGATGCGCAACTGATCCCCGTGCGCAAGATCGTGCTGTTCCAGCAGAAGCAGAAGATCGAGACGGACA
TGGCGCTGTCGGCCGATGCGCGCCGCGCGCAGCTCGCGGCGCTCGATGCGAGGCTCGCCGCGCTCGACGCGTCCGGCGCG
GCGGATGCGTCGAGCGCGCAGGCGGACAAGCGATGA

Upstream 100 bases:

>100_bases
GCCTCGTCCATGCGATGACGCCGCGCGCGCCGCGGGCCGCCCCGCTCGTCGTGCCTGCCCGGCGCGCCATTCGCCCGACC
ACCACAACGGAACCGAACCC

Downstream 100 bases:

>100_bases
ACGCGCGCGAGCCGGCGGGCGCCGCGCTCGGCGAACCGCTCGCAACGGCCGCCGCCGCGCGCCGCGGCTGGCGGGCGTGG
CTTGCGTGGGCGCTCGCGTT

Product: phosphonate ABC transporter, periplasmic phosphonate binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLIRSLVACVAALACAAAAHAQTLNFGIISTDSSAVLKRRWQPLIDDMNRKTGLDVKAFFATDYAGIIEGMRFNKVQLG
YFGNASALEAVERSNGEVFAKLRHANGETGYYSVLITNANSRFRTLDDVFRNPKDVTLGFGDPNSTSGTLVPAYYLFAPR
GIAPRTAFRTVLPSSHEGNLLAVVNDKIDIATNNTDVLGMLKKQHPDQYARVRVLWTSPQIASDPLVWRKDLPDATKQKL
RDFFYNYAKTDPHEQAVMAGIRNLVGFAPSSDAQLIPVRKIVLFQQKQKIETDMALSADARRAQLAALDARLAALDASGA
ADASSAQADKR

Sequences:

>Translated_331_residues
MKLIRSLVACVAALACAAAAHAQTLNFGIISTDSSAVLKRRWQPLIDDMNRKTGLDVKAFFATDYAGIIEGMRFNKVQLG
YFGNASALEAVERSNGEVFAKLRHANGETGYYSVLITNANSRFRTLDDVFRNPKDVTLGFGDPNSTSGTLVPAYYLFAPR
GIAPRTAFRTVLPSSHEGNLLAVVNDKIDIATNNTDVLGMLKKQHPDQYARVRVLWTSPQIASDPLVWRKDLPDATKQKL
RDFFYNYAKTDPHEQAVMAGIRNLVGFAPSSDAQLIPVRKIVLFQQKQKIETDMALSADARRAQLAALDARLAALDASGA
ADASSAQADKR
>Mature_331_residues
MKLIRSLVACVAALACAAAAHAQTLNFGIISTDSSAVLKRRWQPLIDDMNRKTGLDVKAFFATDYAGIIEGMRFNKVQLG
YFGNASALEAVERSNGEVFAKLRHANGETGYYSVLITNANSRFRTLDDVFRNPKDVTLGFGDPNSTSGTLVPAYYLFAPR
GIAPRTAFRTVLPSSHEGNLLAVVNDKIDIATNNTDVLGMLKKQHPDQYARVRVLWTSPQIASDPLVWRKDLPDATKQKL
RDFFYNYAKTDPHEQAVMAGIRNLVGFAPSSDAQLIPVRKIVLFQQKQKIETDMALSADARRAQLAALDARLAALDASGA
ADASSAQADKR

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=324, Percent_Identity=46.6049382716049, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36221; Mature: 36221

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIRSLVACVAALACAAAAHAQTLNFGIISTDSSAVLKRRWQPLIDDMNRKTGLDVKAF
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEE
FATDYAGIIEGMRFNKVQLGYFGNASALEAVERSNGEVFAKLRHANGETGYYSVLITNAN
ECCCHHHHHCCCEECEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCC
SRFRTLDDVFRNPKDVTLGFGDPNSTSGTLVPAYYLFAPRGIAPRTAFRTVLPSSHEGNL
CCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCE
LAVVNDKIDIATNNTDVLGMLKKQHPDQYARVRVLWTSPQIASDPLVWRKDLPDATKQKL
EEEEECEEEEEECCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEECCCCCHHHHHHH
RDFFYNYAKTDPHEQAVMAGIRNLVGFAPSSDAQLIPVRKIVLFQQKQKIETDMALSADA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCH
RRAQLAALDARLAALDASGAADASSAQADKR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLIRSLVACVAALACAAAAHAQTLNFGIISTDSSAVLKRRWQPLIDDMNRKTGLDVKAF
CHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEE
FATDYAGIIEGMRFNKVQLGYFGNASALEAVERSNGEVFAKLRHANGETGYYSVLITNAN
ECCCHHHHHCCCEECEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEECCC
SRFRTLDDVFRNPKDVTLGFGDPNSTSGTLVPAYYLFAPRGIAPRTAFRTVLPSSHEGNL
CCHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCE
LAVVNDKIDIATNNTDVLGMLKKQHPDQYARVRVLWTSPQIASDPLVWRKDLPDATKQKL
EEEEECEEEEEECCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCEECCCCCHHHHHHH
RDFFYNYAKTDPHEQAVMAGIRNLVGFAPSSDAQLIPVRKIVLFQQKQKIETDMALSADA
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCH
RRAQLAALDARLAALDASGAADASSAQADKR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]