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The map label for this gene is glcF [H]

Identifier: 237813699

GI number: 237813699

Start: 3365224

End: 3366450

Strand: Direct

Name: glcF [H]

Synonym: GBP346_A3476

Alternate gene names: 237813699

Gene position: 3365224-3366450 (Clockwise)

Preceding gene: 237813698

Following gene: 237813700

Centisome position: 82.11

GC content: 69.44

Gene sequence:

>1227_bases
ATGCAGACGAACCTCGCCGATTTCATCCGCCACACGCCCGACGGCGACGACGCCGACGCGATCCTGCGCAGGTGCGTGCA
CTGCGGCTTCTGCACCGCGACCTGCCCGACCTACCAGTTGCTCGGCGACGAGCTCGACGGCCCGCGCGGACGCATCTACC
TGATCAAGCAGATGCTCGAGGGCGCGGAAGTCACGCGCAGCACGCAACAGCACCTCGACCGCTGCCTCACGTGCCGCAGT
TGCGAGACGACCTGCCCGTCGGGCGTCGAGTACGGCCGGCTCGTCGACATCGGCCGCAAGGTCGTCGAGACGAAAGTGTC
GCGGCCGCTGTCGCAGCGGATCGTGCGGCGCCTGCTCGCGAGCTTCGTGCCGAACGCGGCCGTGTTCGCGCCCGTGATGC
GGCTCGGCCAGCATGTGCGGCCGCTGTTGCCGAAGCGGCTGCGCGACAAGGTGCCGCCGCGCGCGCGCCTGCTCGCGTGG
CCGAGCGCGCGGCGCGAGCGCCGCGTGCTGATGCTCGCGGGCTGCGTGCAGCCTTCGATGATGCCGAACATCAACATCGC
GACCGCGCGCGTGCTGGATGCGCTCGGCATCGAAACCGTGGTCGCGCCGGAAGCCGGCTGCTGCGGCGCGATCCGGCTGC
ATCTGGGCTATCACGATGAAGCGCTCGACGATGCGCGACGCAACATCGACGCGTGGTGGCCGCACATCGAGCGCGGCGTC
GAAGCGATCGTGATGAACGCGTCCGGCTGCGGCGCGACGGTGCGCGAGTACGCGCACCTGCTGCGCGGCGATCCGGCCTA
CGCGGACAAGGCGCGCCGCATCGTCGAGCTGACGAAGGACGCATCCGAGCTGCTCGGCGGCTTCGAGGCCGAGCTGATCG
CGCTCACGCGCCGGCGCGGAATCCATACCGTCGCCTATCATCCGCCGTGCACGCTGCAGCACGGCCAGCAGTTGCGCGGC
AAGGTCGAGCGGCTGCTGGAGGGGCTCGGCATCGAAGTTCGGCTGCCCGCCGACAGCCATCTGTGCTGCGGCTCCGCGGG
CACCTATTCGCTCACGCAGCCGTCGCTCGCGTACAAGCTGCGCAAGCGCAAGCTCGCGAAGCTGCAGGAGACCGAGCCGC
AGATGATCGTATCGGCGAACATCGGCTGCATCGCGCATCTGCAAAGCGGCACGGCGGTGCCCGTCGTGCACTGGGTGCAG
CTCGTCGAGAATCTGCTGTACGGTTGA

Upstream 100 bases:

>100_bases
GCCCGCGCCGCTGATGAAAATCCACCGCGGCCTGAAGACGGCGTTCGATCCGGCGCGCATCTTCAACCGCGGCCGGCTCT
ACCCCGATCTCTAAAGCGCG

Downstream 100 bases:

>100_bases
CGCCGGACGGCACATGCCGCCGCACGCGTCCGGCCGCTCGCGCCGGTTGTTCGCGCCCGGTTGTTCGCGCCCGGTTGTTC
GCACGGGTTGTTCGCACGGG

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRHTPDGDDADAILRRCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLSQRIVRRLLASFVPNAAVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PSARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHIERGV
EAIVMNASGCGATVREYAHLLRGDPAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEGLGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKRKLAKLQETEPQMIVSANIGCIAHLQSGTAVPVVHWVQ
LVENLLYG

Sequences:

>Translated_408_residues
MQTNLADFIRHTPDGDDADAILRRCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLSQRIVRRLLASFVPNAAVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PSARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHIERGV
EAIVMNASGCGATVREYAHLLRGDPAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEGLGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKRKLAKLQETEPQMIVSANIGCIAHLQSGTAVPVVHWVQ
LVENLLYG
>Mature_408_residues
MQTNLADFIRHTPDGDDADAILRRCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLEGAEVTRSTQQHLDRCLTCRS
CETTCPSGVEYGRLVDIGRKVVETKVSRPLSQRIVRRLLASFVPNAAVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAW
PSARRERRVLMLAGCVQPSMMPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHIERGV
EAIVMNASGCGATVREYAHLLRGDPAYADKARRIVELTKDASELLGGFEAELIALTRRRGIHTVAYHPPCTLQHGQQLRG
KVERLLEGLGIEVRLPADSHLCCGSAGTYSLTQPSLAYKLRKRKLAKLQETEPQMIVSANIGCIAHLQSGTAVPVVHWVQ
LVENLLYG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=57.002457002457, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1788576, Length=410, Percent_Identity=22.9268292682927, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786497, Length=241, Percent_Identity=22.8215767634855, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45156; Mature: 45156

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRHTPDGDDADAILRRCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLE
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHH
GAEVTRSTQQHLDRCLTCRSCETTCPSGVEYGRLVDIGRKVVETKVSRPLSQRIVRRLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SFVPNAAVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAWPSARRERRVLMLAGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCEEEEEECCCCCCC
MPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHIERGV
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCHHHHHHHHHCCC
EAIVMNASGCGATVREYAHLLRGDPAYADKARRIVELTKDASELLGGFEAELIALTRRRG
CEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
IHTVAYHPPCTLQHGQQLRGKVERLLEGLGIEVRLPADSHLCCGSAGTYSLTQPSLAYKL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCCHHHHHHH
RKRKLAKLQETEPQMIVSANIGCIAHLQSGTAVPVVHWVQLVENLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRHTPDGDDADAILRRCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMLE
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCCCCHHHHHHHHHH
GAEVTRSTQQHLDRCLTCRSCETTCPSGVEYGRLVDIGRKVVETKVSRPLSQRIVRRLLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SFVPNAAVFAPVMRLGQHVRPLLPKRLRDKVPPRARLLAWPSARRERRVLMLAGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHCEEEEEECCCCCCC
MPNINIATARVLDALGIETVVAPEAGCCGAIRLHLGYHDEALDDARRNIDAWWPHIERGV
CCCCCHHHHHHHHHHCCHHEECCCCCCCEEEEEEECCCHHHHHHHHHCHHHHHHHHHCCC
EAIVMNASGCGATVREYAHLLRGDPAYADKARRIVELTKDASELLGGFEAELIALTRRRG
CEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCC
IHTVAYHPPCTLQHGQQLRGKVERLLEGLGIEVRLPADSHLCCGSAGTYSLTQPSLAYKL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCCHHHHHHH
RKRKLAKLQETEPQMIVSANIGCIAHLQSGTAVPVVHWVQLVENLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]