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The map label for this gene is rfbA [H]

Identifier: 237813507

GI number: 237813507

Start: 3171679

End: 3172572

Strand: Reverse

Name: rfbA [H]

Synonym: GBP346_A3283

Alternate gene names: 237813507

Gene position: 3172572-3171679 (Counterclockwise)

Preceding gene: 237813508

Following gene: 237813506

Centisome position: 77.41

GC content: 63.42

Gene sequence:

>894_bases
ATGGCGCGCAAGGGCATTATTCTGGCCGGCGGTTCCGGCACGCGGCTGTATCCGATCACGCATGCGGTGTCGAAGCAGCT
GTTGCCGGTCTACGACAAGCCGATGATCTATTACCCGCTGTCGACGCTGATGGTGGCGGACATTCGCGATGTGCTGATCA
TCTCGACGCCGCAGGACACGCCGCGCTTCGAGGCGATGCTGGGTGACGGCAGCCAGTGGGGGATGAACATCCGCTACGCG
GTGCAGCCGTCGCCGGACGGGCTCGCGCAGGCGTTCGTGATCGGCCGGGAATTCGTCGGCCGCGATCCGTCGACGCTGAT
TCTCGGCGACAACATCTTCTATGGCCACGATCTCGCGAAGCAACTCGATCGGGCATCGGCGCGGCAGGCGGGCGCGACGG
TGTTCGCATACCACGTGCACGATCCGGAGCGCTACGGCGTCGTCGAGTTCGACGGCGAATTCCGCGCGCTGTCGATCGAG
GAGAAGCCCGCGAAGCCGCGCTCGAACTACGCGGTGACGGGGCTGTACTTCTACGACAACCAGGTGTGCGACATCGCGGC
GGACATCAAGCCGTCCGCGCGCGGCGAGCTGGAGATCACGGACGTGAATTCCCGCTATCTCGCCGCGGGTTTGCTCGACG
TCGAGATCATGGGCCGCGGCTACGCGTGGCTCGATACCGGCACGCACGATTCGCTGATCGAGGCGGCGACGTTCATCGCG
ACGCTGCAAAAGCGCCAGGGGCTCGTCGTCGCGTGTCCGGAGGAGATCGCGTATCGGCGGCAGTGGATCGACGCGGAACA
ACTGCTCAAGCTCGCACAGCCTTTGTCGAAAAATGGCTATGGCCAGTACCTGAAAAACATTCTTACGGATCAAGTCGCAT
GGCCATCCAAGTAA

Upstream 100 bases:

>100_bases
GGTATCTCGACCATCAGGAGTGGGTCGACGAAGTGGTGTCGGGCGATTACCGCAAGTGGGTCGAAACGAACTACGCGCAA
CGCGCGTAAAGGTTGAACCG

Downstream 100 bases:

>100_bases
CTGCTACCGCTTTGCCGGAAGTCAAGATTATCGAACCGAAGGTATTCGGCGATGCTCGCGGATTCTTCTTCGAGAGCTTC
AATGCTCGAGAATTTACAGA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVADIRDVLIISTPQDTPRFEAMLGDGSQWGMNIRYA
VQPSPDGLAQAFVIGREFVGRDPSTLILGDNIFYGHDLAKQLDRASARQAGATVFAYHVHDPERYGVVEFDGEFRALSIE
EKPAKPRSNYAVTGLYFYDNQVCDIAADIKPSARGELEITDVNSRYLAAGLLDVEIMGRGYAWLDTGTHDSLIEAATFIA
TLQKRQGLVVACPEEIAYRRQWIDAEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK

Sequences:

>Translated_297_residues
MARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVADIRDVLIISTPQDTPRFEAMLGDGSQWGMNIRYA
VQPSPDGLAQAFVIGREFVGRDPSTLILGDNIFYGHDLAKQLDRASARQAGATVFAYHVHDPERYGVVEFDGEFRALSIE
EKPAKPRSNYAVTGLYFYDNQVCDIAADIKPSARGELEITDVNSRYLAAGLLDVEIMGRGYAWLDTGTHDSLIEAATFIA
TLQKRQGLVVACPEEIAYRRQWIDAEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK
>Mature_296_residues
ARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVADIRDVLIISTPQDTPRFEAMLGDGSQWGMNIRYAV
QPSPDGLAQAFVIGREFVGRDPSTLILGDNIFYGHDLAKQLDRASARQAGATVFAYHVHDPERYGVVEFDGEFRALSIEE
KPAKPRSNYAVTGLYFYDNQVCDIAADIKPSARGELEITDVNSRYLAAGLLDVEIMGRGYAWLDTGTHDSLIEAATFIAT
LQKRQGLVVACPEEIAYRRQWIDAEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=72.2222222222222, Blast_Score=446, Evalue=1e-127,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=63.859649122807, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787488, Length=235, Percent_Identity=26.8085106382979, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=191, Percent_Identity=23.5602094240838, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320148, Length=248, Percent_Identity=28.2258064516129, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355443, Length=245, Percent_Identity=22.0408163265306, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24644084, Length=245, Percent_Identity=22.0408163265306, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32963; Mature: 32832

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVADIRDVLIISTPQDT
CCCCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCC
PRFEAMLGDGSQWGMNIRYAVQPSPDGLAQAFVIGREFVGRDPSTLILGDNIFYGHDLAK
CCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEECHHHHH
QLDRASARQAGATVFAYHVHDPERYGVVEFDGEFRALSIEEKPAKPRSNYAVTGLYFYDN
HHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECC
QVCDIAADIKPSARGELEITDVNSRYLAAGLLDVEIMGRGYAWLDTGTHDSLIEAATFIA
CEEEEEECCCCCCCCEEEEEECCCCEEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHH
TLQKRQGLVVACPEEIAYRRQWIDAEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK
HHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVADIRDVLIISTPQDT
CCCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCC
PRFEAMLGDGSQWGMNIRYAVQPSPDGLAQAFVIGREFVGRDPSTLILGDNIFYGHDLAK
CCEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEECHHHHH
QLDRASARQAGATVFAYHVHDPERYGVVEFDGEFRALSIEEKPAKPRSNYAVTGLYFYDN
HHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCEEEEEECCCCCCCCCCEEEEEEEEECC
QVCDIAADIKPSARGELEITDVNSRYLAAGLLDVEIMGRGYAWLDTGTHDSLIEAATFIA
CEEEEEECCCCCCCCEEEEEECCCCEEEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHH
TLQKRQGLVVACPEEIAYRRQWIDAEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK
HHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]