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The map label for this gene is metE [H]

Identifier: 237813355

GI number: 237813355

Start: 3033062

End: 3035356

Strand: Reverse

Name: metE [H]

Synonym: GBP346_A3125

Alternate gene names: 237813355

Gene position: 3035356-3033062 (Counterclockwise)

Preceding gene: 237813360

Following gene: 237813354

Centisome position: 74.06

GC content: 71.02

Gene sequence:

>2295_bases
ATGACCACCGCACACATCCTGGGTTTTCCGCGCATCGGCGCGCAACGGGAACTGAAGTTCGCCCTCGAGCGCTACTGGCG
CGACGGCGCGTCGGCCGACGCCGAGCGCGCGCTCGTCGATACCGGCCGCGCGCTGCGCGCCGAGCACTGGCGCATTGAGC
GCGACGCCGGCCTCGACTGCGTGACGGTCGGCGATTTCGCGTGGTACGACCACGTGCTGACGACGCTCGCGCACGTCGGC
GGCCTGCCGCGGCGTTTCGGCTTCGACGCGCGCGCGCTCACGCTCGCCGACTATTTCGCGGCCGCGCGCGGCAACGCCGC
GCAGCCGGCGATGGAGATGACGAAGTGGTTCGATACGAACTATCACTACCTCGTGCCCGAGTACTCGCCCGCGACGACGT
TCGGGCCGGGCGTCGAGTGGCTCTTCGACGAGGTGCGCGAGGCGCGCGCGCTCGGCCATCGCGCGAAGGCGGCGCTCGTC
GGCCCGCTTACGCTGCTCTGGCTAGGCAAGGCGCGCGACGGGCTCGTCGAGCGCCTCGCGCTGCTGCCGCGCCTCGTGCC
CGCGTATCGCGCGCTGCTCGCGCGGCTGAGGGAAGCGGGCGTCGACTGGGTGCAGATCGACGAGCCGATCTTCTCGCTCG
ATCTGCCCGACGCGTGGCGCGACGCGGCGCGGCCGACGTACGAAGCGCTCGCGCCCGGCGCGCCGAAGCTGCTCGTCGCG
ACGTATTTCGACGACGCGAGCGAGCACGCGGCGCTGCTCAAGGCGCTGCCCGTCGCGGGCCTGCACGTCGATCTCGTGCG
CGCCGACGCGCAGCTCGATGCGTTCGTCGCGGACTATCCGGCCGACAAGGTGCTCTCGTGCGGCATCGTCGACGGCCGCA
ACGTGTGGCGCAACGATCTCGACCGTTCGCTCGCGCGGCTCGCGCCGGTGCGCGACGCGCTCGGCGAGCGGCTGTGGGTC
GCGACGAGCTGCTCGCTGCTGCATGTGCCCGTCGATCTCGCGCATGAGCCGAGGCTCGACGAGGAGCTGAAGACTTGGCT
CGCGTTCGCGGCGCAGAAGACGCGCGAGGTCGCCGCGCTGCGCGACGCGCTCGTGAAGGGGCGCGCGGCGGTCGCGGCCG
AGTTCGATGACGCCGCCGCGGCGGCGGCCGCGCGGCGCACGTCGGCGCGCATTCACAATCCGCTCGTCAAGCGCCGCGTC
GCGGCGCTGACCGACGCCGACGCGCGCCGCGCGAGCACCTATAGCGTGCGTGCGGCCGCGCAGCGCGCGCGCTTCGGCTT
GCCGCTGCTGCCGACGACGACGATCGGCTCGTTTCCGCAGACGCCGGAGATTCGCCGCGCGCGCGCCGCGTTCAAGCAGG
GCGTGCTCGATCACCTCGGCTATCTGGAGGCGATGCGCGAGCAGGTGCGCATCGCGATCGACAAGCAGCTCGCGTACGGC
CTCGACGTGCTCGTGCACGGCGAGGCCGAGCGCAACGACATGGTCGAATACTTCGGCGAACTGCTGTGGGGCTTCGCGAT
CACGAGCAACGGCTGGGTCCAGAGCTACGGTTCGCGCTGCGTGAAGCCGCCCCTCGTTTATGGCGACGTGTATTTGCCGG
AGCCGATGACGGTCGGCTGGGCGTCGTATGCGCAGAGCCTGAGCGCGAAGCCGGTGAAGGGCATGCTGACGGGGCCCGTG
ACGATGCTGCAATGGTCGTTCGTGCGCGACGACCAGCCGCGCGCGACGACGGCCTTGCAGATCGCGCTCGCGCTGCGGCA
GGAGACGCTCGACCTCGAGAAGGCCGGCATCGGGATGATCCAGATCGACGAGCCGGCGCTGCGCGAGGGGTTGCCGCTGA
AGGCGCGCGAGCGCGCCGCGTATCTGGACTGGGCGGTGCGCGCGTTCGGGATCGCGGCGTCGGGCGTCGCGGACGACACG
CAGATCCACACGCACATGTGCTATTCGGAGTTCGGCGACATCCTGCCGTCGATCGCGGCGCTCGACGCGGACGTGATCTC
GATCGAGACGACCCGCTCGAACATGGAACTGCTCGACGCGTTCGAGACGTTCGACTATCCGAACGAGATCGGGCCGGGCG
TCTATGACATCCATTCGCCGCGCGTGCCGGACGCGGACGAGATCGAGCGACTCATCCTGCTCGCGCTCGAGCGGATTCCG
GCGCAGCGCCTGTGGGTGAATCCGGACTGCGGGCTGAAGACGCGCGAGTGGCGGCAGGTCGACGCGGCGCTCGCGGCGAT
GGTCGACGCGGCCAAGCGCGTGCGGCAGAAGGTCGAGGAGGCGGCGCCGGCGTGA

Upstream 100 bases:

>100_bases
GCGAATGATGTTGAAAAAAATTCATGTTGTTCGGCAAAAACTTTCGTTTGAGTAATGATAGGGCAAATCCGATACTGCCG
GTCACTTCTGGAGGATCGAG

Downstream 100 bases:

>100_bases
CGCGGCGCGCGGCGGTGCGAAAGAGGTGTGCGCAGAAGGGGCGTGAATGCGCGTGAAGGGGTGTGAGGGTGCGTGAGGGC
GGCGCGCCGAGCGCGGCGCA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MTTAHILGFPRIGAQRELKFALERYWRDGASADAERALVDTGRALRAEHWRIERDAGLDCVTVGDFAWYDHVLTTLAHVG
GLPRRFGFDARALTLADYFAAARGNAAQPAMEMTKWFDTNYHYLVPEYSPATTFGPGVEWLFDEVREARALGHRAKAALV
GPLTLLWLGKARDGLVERLALLPRLVPAYRALLARLREAGVDWVQIDEPIFSLDLPDAWRDAARPTYEALAPGAPKLLVA
TYFDDASEHAALLKALPVAGLHVDLVRADAQLDAFVADYPADKVLSCGIVDGRNVWRNDLDRSLARLAPVRDALGERLWV
ATSCSLLHVPVDLAHEPRLDEELKTWLAFAAQKTREVAALRDALVKGRAAVAAEFDDAAAAAAARRTSARIHNPLVKRRV
AALTDADARRASTYSVRAAAQRARFGLPLLPTTTIGSFPQTPEIRRARAAFKQGVLDHLGYLEAMREQVRIAIDKQLAYG
LDVLVHGEAERNDMVEYFGELLWGFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGWASYAQSLSAKPVKGMLTGPV
TMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMIQIDEPALREGLPLKARERAAYLDWAVRAFGIAASGVADDT
QIHTHMCYSEFGDILPSIAALDADVISIETTRSNMELLDAFETFDYPNEIGPGVYDIHSPRVPDADEIERLILLALERIP
AQRLWVNPDCGLKTREWRQVDAALAAMVDAAKRVRQKVEEAAPA

Sequences:

>Translated_764_residues
MTTAHILGFPRIGAQRELKFALERYWRDGASADAERALVDTGRALRAEHWRIERDAGLDCVTVGDFAWYDHVLTTLAHVG
GLPRRFGFDARALTLADYFAAARGNAAQPAMEMTKWFDTNYHYLVPEYSPATTFGPGVEWLFDEVREARALGHRAKAALV
GPLTLLWLGKARDGLVERLALLPRLVPAYRALLARLREAGVDWVQIDEPIFSLDLPDAWRDAARPTYEALAPGAPKLLVA
TYFDDASEHAALLKALPVAGLHVDLVRADAQLDAFVADYPADKVLSCGIVDGRNVWRNDLDRSLARLAPVRDALGERLWV
ATSCSLLHVPVDLAHEPRLDEELKTWLAFAAQKTREVAALRDALVKGRAAVAAEFDDAAAAAAARRTSARIHNPLVKRRV
AALTDADARRASTYSVRAAAQRARFGLPLLPTTTIGSFPQTPEIRRARAAFKQGVLDHLGYLEAMREQVRIAIDKQLAYG
LDVLVHGEAERNDMVEYFGELLWGFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGWASYAQSLSAKPVKGMLTGPV
TMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMIQIDEPALREGLPLKARERAAYLDWAVRAFGIAASGVADDT
QIHTHMCYSEFGDILPSIAALDADVISIETTRSNMELLDAFETFDYPNEIGPGVYDIHSPRVPDADEIERLILLALERIP
AQRLWVNPDCGLKTREWRQVDAALAAMVDAAKRVRQKVEEAAPA
>Mature_763_residues
TTAHILGFPRIGAQRELKFALERYWRDGASADAERALVDTGRALRAEHWRIERDAGLDCVTVGDFAWYDHVLTTLAHVGG
LPRRFGFDARALTLADYFAAARGNAAQPAMEMTKWFDTNYHYLVPEYSPATTFGPGVEWLFDEVREARALGHRAKAALVG
PLTLLWLGKARDGLVERLALLPRLVPAYRALLARLREAGVDWVQIDEPIFSLDLPDAWRDAARPTYEALAPGAPKLLVAT
YFDDASEHAALLKALPVAGLHVDLVRADAQLDAFVADYPADKVLSCGIVDGRNVWRNDLDRSLARLAPVRDALGERLWVA
TSCSLLHVPVDLAHEPRLDEELKTWLAFAAQKTREVAALRDALVKGRAAVAAEFDDAAAAAAARRTSARIHNPLVKRRVA
ALTDADARRASTYSVRAAAQRARFGLPLLPTTTIGSFPQTPEIRRARAAFKQGVLDHLGYLEAMREQVRIAIDKQLAYGL
DVLVHGEAERNDMVEYFGELLWGFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGWASYAQSLSAKPVKGMLTGPVT
MLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMIQIDEPALREGLPLKARERAAYLDWAVRAFGIAASGVADDTQ
IHTHMCYSEFGDILPSIAALDADVISIETTRSNMELLDAFETFDYPNEIGPGVYDIHSPRVPDADEIERLILLALERIPA
QRLWVNPDCGLKTREWRQVDAALAAMVDAAKRVRQKVEEAAPA

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=757, Percent_Identity=57.5957727873184, Blast_Score=840, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=776, Percent_Identity=46.2628865979381, Blast_Score=642, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84369; Mature: 84238

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAHILGFPRIGAQRELKFALERYWRDGASADAERALVDTGRALRAEHWRIERDAGLDC
CCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHCEEECCCCCCE
VTVGDFAWYDHVLTTLAHVGGLPRRFGFDARALTLADYFAAARGNAAQPAMEMTKWFDTN
EEECCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
YHYLVPEYSPATTFGPGVEWLFDEVREARALGHRAKAALVGPLTLLWLGKARDGLVERLA
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLPRLVPAYRALLARLREAGVDWVQIDEPIFSLDLPDAWRDAARPTYEALAPGAPKLLVA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHCCCCHHHHCCCCCCEEEE
TYFDDASEHAALLKALPVAGLHVDLVRADAQLDAFVADYPADKVLSCGIVDGRNVWRNDL
EECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHHEEECCCCCCHHHHHHH
DRSLARLAPVRDALGERLWVATSCSLLHVPVDLAHEPRLDEELKTWLAFAAQKTREVAAL
HHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDALVKGRAAVAAEFDDAAAAAAARRTSARIHNPLVKRRVAALTDADARRASTYSVRAAA
HHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QRARFGLPLLPTTTIGSFPQTPEIRRARAAFKQGVLDHLGYLEAMREQVRIAIDKQLAYG
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDVLVHGEAERNDMVEYFGELLWGFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGW
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCEECCCCCCCCH
ASYAQSLSAKPVKGMLTGPVTMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMI
HHHHHHCCCCCCCCHHCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
QIDEPALREGLPLKARERAAYLDWAVRAFGIAASGVADDTQIHTHMCYSEFGDILPSIAA
EECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDADVISIETTRSNMELLDAFETFDYPNEIGPGVYDIHSPRVPDADEIERLILLALERIP
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCC
AQRLWVNPDCGLKTREWRQVDAALAAMVDAAKRVRQKVEEAAPA
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTAHILGFPRIGAQRELKFALERYWRDGASADAERALVDTGRALRAEHWRIERDAGLDC
CCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHCEEECCCCCCE
VTVGDFAWYDHVLTTLAHVGGLPRRFGFDARALTLADYFAAARGNAAQPAMEMTKWFDTN
EEECCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
YHYLVPEYSPATTFGPGVEWLFDEVREARALGHRAKAALVGPLTLLWLGKARDGLVERLA
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LLPRLVPAYRALLARLREAGVDWVQIDEPIFSLDLPDAWRDAARPTYEALAPGAPKLLVA
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHCCCCHHHHCCCCCCEEEE
TYFDDASEHAALLKALPVAGLHVDLVRADAQLDAFVADYPADKVLSCGIVDGRNVWRNDL
EECCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCHHEEECCCCCCHHHHHHH
DRSLARLAPVRDALGERLWVATSCSLLHVPVDLAHEPRLDEELKTWLAFAAQKTREVAAL
HHHHHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RDALVKGRAAVAAEFDDAAAAAAARRTSARIHNPLVKRRVAALTDADARRASTYSVRAAA
HHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
QRARFGLPLLPTTTIGSFPQTPEIRRARAAFKQGVLDHLGYLEAMREQVRIAIDKQLAYG
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDVLVHGEAERNDMVEYFGELLWGFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGW
CEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCEECCCCCCCCH
ASYAQSLSAKPVKGMLTGPVTMLQWSFVRDDQPRATTALQIALALRQETLDLEKAGIGMI
HHHHHHCCCCCCCCHHCCCHHHHEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
QIDEPALREGLPLKARERAAYLDWAVRAFGIAASGVADDTQIHTHMCYSEFGDILPSIAA
EECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LDADVISIETTRSNMELLDAFETFDYPNEIGPGVYDIHSPRVPDADEIERLILLALERIP
CCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCC
AQRLWVNPDCGLKTREWRQVDAALAAMVDAAKRVRQKVEEAAPA
HHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA