Click here to switch to the map view.

The map label for this gene is nuoF2 [H]

Identifier: 237813331

GI number: 237813331

Start: 3007393

End: 3008958

Strand: Direct

Name: nuoF2 [H]

Synonym: GBP346_A3100

Alternate gene names: 237813331

Gene position: 3007393-3008958 (Clockwise)

Preceding gene: 237813330

Following gene: 237813332

Centisome position: 73.38

GC content: 71.46

Gene sequence:

>1566_bases
ATGAGCACGCGCATTCATGTGCCGCGCGATTCGGCCGCGCTCGCGGTCGGCGCCGACGCGCTCGCGCGCGCGATCGAGGC
GCAGGCGGCCGCGCGCGGCATCGCGATCGAGCTCGTGCGCAACGGCTCGCGCGGGCTGCTGTGGCTCGAGCCGCTCGTCG
AAGTGAGCACCCCGGCGGGCCGCATCGGCTACGCGAACCTCACGGCAGCCGACGTCCCCGCGCTCTTCGACGCGGGCTGG
CTCGGCGGCGCCGCGCATCCGGCGTGCGTCGGCATCGTCGACGAGATTCCCTATCTGAAGCACCAGCAGCGGCTCACGTT
CGCGCGCATCGGCATCACCGATCCGCTGTCGATCGACGATTACGTCGCGCACGGCGGCCTCGAAGGCCTGAGGAACGCGC
TCGCACTCGACGGCGCCGCCGCATGCGAGACGCTCGTCGAATCGGGCCTGCGCGGGCGCGGCGGCGCCGCATTCCCGGCG
GGCGTCAAATGGCGGACCGTGCGCGAGGCCGCGGCCGCGCAGAAATACGTCGTCTGCAATGCGGACGAAGGCGACTCCGG
CACCTTCTCCGATCGCCTCGTGATGGAAAGCGATCCGTACTGCCTGATCGAAGGAATGATCATCGCGGGCATCGCGACGG
GCGCGACGATCGGCCATATCTACGTGCGCAGCGAATATCCGCACGCGATCGCGGCGCTCGGCGAGGCGATCGCCCGCGCG
CGCGCGGCCGGCTGGCTCGGCGCGAGCGTGCTCGGCACCGCGCATGCGTTCGAGCTGCACGTCGCGAAAGGCGCGGGCGC
CTACGTCTGCGGCGAAGAGACCGCGCTCCTCGAATCGCTCGAAGGCAAGCGCGGCGTCGTGCGCGCGAAGCCGCCGCTGC
CCGCGCTATCGGGACTCTTCGCAAAACCGACCGTCATCAACAACGTGATCACGCTCGCGACCGTACCGATCATCTTCGCG
CGCGGCGCGGCGTTCTATCGCGATTACGGCATGGGCCGCTCGCGCGGCACGCTGCCGTTCCAGCTCGCAGGCAACGTGAA
GCGCGGCGGGCTCGTCGAGCTCGCGTTCGGCGTCACGCTGCGCGAGCTGCTGTTCGATTTCGGCGGCGGCACCGCGAGCG
GGCGCCCCGCGCGCGCCGCGCAGGTCGGCGGCCCGCTCGGCACCTACCTGCCCGAGAGCCAGTGGGACATCCCGCTCGAT
TACGAGGCGTACGCGGCTGTCGGCGCCGTCGTCGGGCACGGCGGCATCGTGCTGCACGACGACACGTCGAACCTGGCCGA
GCTCGCCGAATACGCGATGCACTTCTGCGCGCTCGAATCGTGCGGCAAGTGCACGCCGTGCCGGATCGGCTCGACGCGCG
GCGTCGAGACGATCGCGAGGATCCGCGCGGGCGACACGTCGGAGCGACAGGTGCGCCTGCTGCGCGACCTGTGCGACACG
ATGACCGCGGGCTCGCTCTGCGCGATGGGCGGCATGACGCCGTACCCGGTGCTGTCCGCGCTCGACCATTTCCCCGAAGA
TTTCGGCCTCGCCGCGCACGCGAGCCACGCGGCACCCGCCGCCTGA

Upstream 100 bases:

>100_bases
AGTCGCCGTCGCTCACGATCAACGACGAGCCGCACGCGAAGATGTCGCCCGCGCGCTTCGACGCGCTCTTCGACGCGGCC
GTCCGCACGAAGGAGCCCGC

Downstream 100 bases:

>100_bases
CAGCAGGAGCTCCGATGACCTCTTCCGCATTCGATTCGTCCCGGCAAGGCTGCGGCTCCGGCCAGTGCGCGTGCCGGCGC
GCCGCGCAGACGCGGCGCGC

Product: NAD-dependent formate dehydrogenase beta subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSTRIHVPRDSAALAVGADALARAIEAQAAARGIAIELVRNGSRGLLWLEPLVEVSTPAGRIGYANLTAADVPALFDAGW
LGGAAHPACVGIVDEIPYLKHQQRLTFARIGITDPLSIDDYVAHGGLEGLRNALALDGAAACETLVESGLRGRGGAAFPA
GVKWRTVREAAAAQKYVVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARA
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALSGLFAKPTVINNVITLATVPIIFA
RGAAFYRDYGMGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPESQWDIPLD
YEAYAAVGAVVGHGGIVLHDDTSNLAELAEYAMHFCALESCGKCTPCRIGSTRGVETIARIRAGDTSERQVRLLRDLCDT
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAAHASHAAPAA

Sequences:

>Translated_521_residues
MSTRIHVPRDSAALAVGADALARAIEAQAAARGIAIELVRNGSRGLLWLEPLVEVSTPAGRIGYANLTAADVPALFDAGW
LGGAAHPACVGIVDEIPYLKHQQRLTFARIGITDPLSIDDYVAHGGLEGLRNALALDGAAACETLVESGLRGRGGAAFPA
GVKWRTVREAAAAQKYVVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARA
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALSGLFAKPTVINNVITLATVPIIFA
RGAAFYRDYGMGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPESQWDIPLD
YEAYAAVGAVVGHGGIVLHDDTSNLAELAEYAMHFCALESCGKCTPCRIGSTRGVETIARIRAGDTSERQVRLLRDLCDT
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAAHASHAAPAA
>Mature_520_residues
STRIHVPRDSAALAVGADALARAIEAQAAARGIAIELVRNGSRGLLWLEPLVEVSTPAGRIGYANLTAADVPALFDAGWL
GGAAHPACVGIVDEIPYLKHQQRLTFARIGITDPLSIDDYVAHGGLEGLRNALALDGAAACETLVESGLRGRGGAAFPAG
VKWRTVREAAAAQKYVVCNADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARAR
AAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALSGLFAKPTVINNVITLATVPIIFAR
GAAFYRDYGMGRSRGTLPFQLAGNVKRGGLVELAFGVTLRELLFDFGGGTASGRPARAAQVGGPLGTYLPESQWDIPLDY
EAYAAVGAVVGHGGIVLHDDTSNLAELAEYAMHFCALESCGKCTPCRIGSTRGVETIARIRAGDTSERQVRLLRDLCDTM
TAGSLCAMGGMTPYPVLSALDHFPEDFGLAAHASHAAPAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=363, Percent_Identity=37.1900826446281, Blast_Score=243, Evalue=5e-64,
Organism=Homo sapiens, GI20149568, Length=363, Percent_Identity=37.1900826446281, Blast_Score=242, Evalue=6e-64,
Organism=Escherichia coli, GI1788620, Length=407, Percent_Identity=39.8034398034398, Blast_Score=293, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI17531727, Length=366, Percent_Identity=39.0710382513661, Blast_Score=255, Evalue=5e-68,
Organism=Drosophila melanogaster, GI19920794, Length=363, Percent_Identity=38.0165289256198, Blast_Score=252, Evalue=6e-67,
Organism=Drosophila melanogaster, GI45550452, Length=363, Percent_Identity=38.2920110192837, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24653819, Length=352, Percent_Identity=31.5340909090909, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=29.5180722891566, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54234; Mature: 54103

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRIHVPRDSAALAVGADALARAIEAQAAARGIAIELVRNGSRGLLWLEPLVEVSTPAG
CCCEEECCCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHHCCCCCC
RIGYANLTAADVPALFDAGWLGGAAHPACVGIVDEIPYLKHQQRLTFARIGITDPLSIDD
CCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCEEEEECCCCCCCCHH
YVAHGGLEGLRNALALDGAAACETLVESGLRGRGGAAFPAGVKWRTVREAAAAQKYVVCN
HHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
ADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARA
CCCCCCCCCCCEEEECCCCEEEEECCCEEHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALSGLF
HHHHHHHHHHHHCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCC
AKPTVINNVITLATVPIIFARGAAFYRDYGMGRSRGTLPFQLAGNVKRGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPESQWDIPLDYEAYAAVGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMHFCALESCGKCTPCRIGSTRGVETIARIRAGDTSERQVRLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAAHASHAAPAA
HCCCCHHHHCCCCHHHHHHHHHHCCHHCCCHHCCCCCCCCC
>Mature Secondary Structure 
STRIHVPRDSAALAVGADALARAIEAQAAARGIAIELVRNGSRGLLWLEPLVEVSTPAG
CCEEECCCCCCEEEECHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECHHHHCCCCCC
RIGYANLTAADVPALFDAGWLGGAAHPACVGIVDEIPYLKHQQRLTFARIGITDPLSIDD
CCCCCCCCHHHCCHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHCCEEEEECCCCCCCCHH
YVAHGGLEGLRNALALDGAAACETLVESGLRGRGGAAFPAGVKWRTVREAAAAQKYVVCN
HHHHCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEE
ADEGDSGTFSDRLVMESDPYCLIEGMIIAGIATGATIGHIYVRSEYPHAIAALGEAIARA
CCCCCCCCCCCEEEECCCCEEEEECCCEEHHHCCCEEEEEEEECCCCHHHHHHHHHHHHH
RAAGWLGASVLGTAHAFELHVAKGAGAYVCGEETALLESLEGKRGVVRAKPPLPALSGLF
HHHHHHHHHHHHCCEEEEEEEECCCCEEEECCHHHHHHHCCCCCCEEECCCCCHHHHCCC
AKPTVINNVITLATVPIIFARGAAFYRDYGMGRSRGTLPFQLAGNVKRGGLVELAFGVTL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEHHHHHH
RELLFDFGGGTASGRPARAAQVGGPLGTYLPESQWDIPLDYEAYAAVGAVVGHGGIVLHD
HHHHHHCCCCCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEC
DTSNLAELAEYAMHFCALESCGKCTPCRIGSTRGVETIARIRAGDTSERQVRLLRDLCDT
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MTAGSLCAMGGMTPYPVLSALDHFPEDFGLAAHASHAAPAA
HCCCCHHHHCCCCHHHHHHHHHHCCHHCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]