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The map label for this gene is trmD

Identifier: 237813270

GI number: 237813270

Start: 2950908

End: 2951702

Strand: Reverse

Name: trmD

Synonym: GBP346_A3037

Alternate gene names: 237813270

Gene position: 2951702-2950908 (Counterclockwise)

Preceding gene: 237813271

Following gene: 237813269

Centisome position: 72.02

GC content: 67.55

Gene sequence:

>795_bases
ATGGACGAAGCCACGCAGAGCGCGATCCAGTTCGACGTCGTCACACTCTTTCCGGAGATGTTTCGCGCGTTGACCGACTG
GGGCATCACGAGCCGCGCGGTGAAGCAGGGGCGGTTCGGCTTGCGCACGTGGAATCCGCGCGATTTCACGACCGACAACT
ACCGCACCGTCGACGATCGCCCGTACGGCGGCGGCCCCGGCATGGTGATGCTCGCGAAACCGCTCGAGGCGGCGATCGGC
GCGGCGAAGGCCGCGCAGGCGGCGCAAGGCGTCGCGACGTCGCGCGTCGTGATGATGTCGCCGCAGGGCGCGCCGCTCAC
GCACGAGCGCGTCGCGCGGATGGCGGCGGAGCCCGGCGTCGTGCTGCTGTGCGGACGTTACGAGGCGATCGATCAACGGT
TGATCGACCGTTGTGTCGACGAGGAACTGAGCCTCGGCGATTTCGTGCTGTCGGGCGGCGAATTGCCGGCGATGGCGCTG
ATGGACGCCGTCGTGCGACTGCTGCCCGGTGTGCTGAATGATGCGCAATCGGCGGTGCAGGACAGCTTCGCCGATGGGTT
GCTCGATTGCCCGCATTACACGCGGCCCGAGGAGTACGAGGGCGTGCGCGTGCCGGACGTGCTGCTCGGCGGGCATCATG
CGGAGATCGAGCGCTGGCGTCGGCAGGAAGCGTTGAGAAACACCATCGCGAAGCGCCCCGACCTGATCGCGCGGGCGCGT
CGCGAAAAGTTGCTGAGCCGGGCCGACGAGGCATGGCTTGCAAGTCTCGCGAAAGAAGCGAAGCAGGCCTCCTGA

Upstream 100 bases:

>100_bases
AAAACCGGCGAGCGGCTGATTCCGTTCGTCGGCGTGTACGTGAAGGCGGTCGAGCAGGCGGCGGGGCGCGTCGTCGTCGA
CTGGGAAGCCGATTACTGAA

Downstream 100 bases:

>100_bases
GGCGGCGGTGCTTCGTTCGCGAATTAGGCGGTGCCGCGCATGCGTGCGCGGCGCCTGATGTGAATCCATCCTCTATCGGG
GCCGAGCCTGGGAGCAGGCG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDEATQSAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTIAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS

Sequences:

>Translated_264_residues
MDEATQSAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTIAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS
>Mature_264_residues
MDEATQSAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMLAKPLEAAIG
AAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGVVLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMAL
MDAVVRLLPGVLNDAQSAVQDSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTIAKRPDLIARAR
REKLLSRADEAWLASLAKEAKQAS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=57.1428571428571, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BURM7 (A3MHR9)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001079805.1
- ProteinModelPortal:   A3MHR9
- SMR:   A3MHR9
- GeneID:   4894665
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_0230
- TIGR:   BMA10247_0230
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   BMAL320389:BMA10247_0230-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29000; Mature: 29000

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEATQSAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGV
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMALMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTIAKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLASLAKEAKQAS
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDEATQSAIQFDVVTLFPEMFRALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMLAKPLEAAIGAAKAAQAAQGVATSRVVMMSPQGAPLTHERVARMAAEPGV
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLIDRCVDEELSLGDFVLSGGELPAMALMDAVVRLLPGVLNDAQSAVQ
EEEECCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DSFADGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIERWRRQEALRNTIAKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
REKLLSRADEAWLASLAKEAKQAS
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA