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The map label for this gene is pmbA [H]

Identifier: 237813257

GI number: 237813257

Start: 2940333

End: 2941703

Strand: Reverse

Name: pmbA [H]

Synonym: GBP346_A3024

Alternate gene names: 237813257

Gene position: 2941703-2940333 (Counterclockwise)

Preceding gene: 237813258

Following gene: 237813256

Centisome position: 71.77

GC content: 69.07

Gene sequence:

>1371_bases
ATGGCTGCAAACCTCGACGCCCAGGCGCACTATTTTCCGCACACGCAAGACGAACTGAAAGCGATCGCGACGGACATCCT
CCGGCACGCGAAGGCGCTCGGCGCGACCGACGCCGCCACCGAAATCTCCGAGGGCGACGGCCTGTCGGTGTCGGTGCGGC
GCGGCGAGGTCGAGACGATCGAGCACAACCGCGACAAGACGGTCGGCGTGACCGTCTTCATCGGCAAGAAGCGGGGCAAC
GCGAGCACGTCGGATTTCTCGCCCACGGCGCTCAAGGATACGGTCGCCGCCGCGTACAACATCGCGCGCTTCACGGCCGA
GGACGACGCGGCAGGGCTCGCCGAAGCCGAACTGCTCGAAACCGAGCCGCGCGACCTGGACCTTTATCACCCGTGGCGCC
TGTCGGCCGACGAAGCGGTCGATCTCGCGCGCCGCTCGGAGGACGCCGCTTTCGCGGTGAGCCCGCAGATCCGCAATTCG
GAGGGCGCGAGCGTGTCCGCGCAGCATTCGCAGTTCGTGCTCGCGACGACGCGCGGCTTTCTCGCAGGCTATCCGTATTC
GCGCCACTACATCGCGTGCGCGCCGATCGCGGGCAGCGGCCGCCACATGCAGCGCGACGACTGGTACACGTCCAAGCGCC
GCGCGGACGAGTTGGCGTCGCCCGAATCGGTCGGCCGCTACGCGGCGCAACGCGCGCTCGCGCGGATGGGCGCGCGCCGC
CTCGACACGCGCAAGGTGCCGGTCCTCTTCGAGGCGCCGCTCGCGGCGGGCCTGCTCGGCGCGTTCGTGCAGGCGGTGAG
CGGCGGCGCGCTGTACCGCAAGACGTCGTTCCTCGTCGACAGCCTCGGCAAGGAGGTGTTCGCGCCGCACGTGCAGATCG
TCGAGGATCCGCACGTGCCGCGCGCGATGGGCAGCGCGCCGTTCGACGAGGAGGGCGTGCGCACGCGCGCGCGCAGCGTC
GTCCGGGACGGCGTGGTCGAGGGCTATTTCCTGTCGACGTATTCGGCGCGCAAGCTCGGCACGCAGACGACCGGCAACGC
GGGCGGCTCGCACAACATCGCGCTGCGCAGCGCGCTCACGGCGCCCGGCGACGATTTCGACGCGATGCTCAAGAAGCTCG
GCACGGGACTTTTGCTGACCGAACTGATGGGGCAGGGCGTCAACTACGTGACGGGCGACTACTCGCGCGGCGCGGCGGGC
TTCTGGGTCGAGAACGGCGAGATCCAGTATCCGGTCGAGGAGATCACGGTGGCGAGCACGCTGCAGGAGATGTTCCGCCA
TATCGTCGCGATCGGCGCGGATTCGATCGTGCGCGGCACGAAGGAAACGGGTTCGGTGCTGATCGAGCAGATGACGATCG
CGGGGCAGTAA

Upstream 100 bases:

>100_bases
GTCGCCGACCCGCGTCGCGCGCGCCGGACCTTGCTATGATCGCGGAATACGCACATTTCGAAACACGCGGGCGACCCGCC
CGTCACCGGATACCACGACG

Downstream 100 bases:

>100_bases
GCGCTCCGATCGCTGCATCCGTTTCGGTCGTCTCGATCGATCCGATCGCAAAAGCAAAAGGCGCCGCGGCAAGCGGCGCC
TCAGATTGCTGACAAACCCT

Product: TldD/PmbA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAANLDAQAHYFPHTQDELKAIATDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKTVGVTVFIGKKRGN
ASTSDFSPTALKDTVAAAYNIARFTAEDDAAGLAEAELLETEPRDLDLYHPWRLSADEAVDLARRSEDAAFAVSPQIRNS
EGASVSAQHSQFVLATTRGFLAGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRRADELASPESVGRYAAQRALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKEVFAPHVQIVEDPHVPRAMGSAPFDEEGVRTRARSV
VRDGVVEGYFLSTYSARKLGTQTTGNAGGSHNIALRSALTAPGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGEIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Sequences:

>Translated_456_residues
MAANLDAQAHYFPHTQDELKAIATDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKTVGVTVFIGKKRGN
ASTSDFSPTALKDTVAAAYNIARFTAEDDAAGLAEAELLETEPRDLDLYHPWRLSADEAVDLARRSEDAAFAVSPQIRNS
EGASVSAQHSQFVLATTRGFLAGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRRADELASPESVGRYAAQRALARMGARR
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKEVFAPHVQIVEDPHVPRAMGSAPFDEEGVRTRARSV
VRDGVVEGYFLSTYSARKLGTQTTGNAGGSHNIALRSALTAPGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAG
FWVENGEIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
>Mature_455_residues
AANLDAQAHYFPHTQDELKAIATDILRHAKALGATDAATEISEGDGLSVSVRRGEVETIEHNRDKTVGVTVFIGKKRGNA
STSDFSPTALKDTVAAAYNIARFTAEDDAAGLAEAELLETEPRDLDLYHPWRLSADEAVDLARRSEDAAFAVSPQIRNSE
GASVSAQHSQFVLATTRGFLAGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRRADELASPESVGRYAAQRALARMGARRL
DTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKEVFAPHVQIVEDPHVPRAMGSAPFDEEGVRTRARSVV
RDGVVEGYFLSTYSARKLGTQTTGNAGGSHNIALRSALTAPGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGF
WVENGEIQYPVEEITVASTLQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=426, Percent_Identity=47.6525821596244, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1789640, Length=401, Percent_Identity=22.6932668329177, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 49034; Mature: 48903

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANLDAQAHYFPHTQDELKAIATDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCEEEH
EHNRDKTVGVTVFIGKKRGNASTSDFSPTALKDTVAAAYNIARFTAEDDAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
TEPRDLDLYHPWRLSADEAVDLARRSEDAAFAVSPQIRNSEGASVSAQHSQFVLATTRGF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCEEEEEECCHH
LAGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRRADELASPESVGRYAAQRALARMGARR
HHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKEVFAPHVQIVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
RAMGSAPFDEEGVRTRARSVVRDGVVEGYFLSTYSARKLGTQTTGNAGGSHNIALRSALT
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCC
APGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGEIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCEEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AANLDAQAHYFPHTQDELKAIATDILRHAKALGATDAATEISEGDGLSVSVRRGEVETI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEECCCEEEH
EHNRDKTVGVTVFIGKKRGNASTSDFSPTALKDTVAAAYNIARFTAEDDAAGLAEAELLE
HCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHC
TEPRDLDLYHPWRLSADEAVDLARRSEDAAFAVSPQIRNSEGASVSAQHSQFVLATTRGF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCEEEEEECCHH
LAGYPYSRHYIACAPIAGSGRHMQRDDWYTSKRRADELASPESVGRYAAQRALARMGARR
HHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDTRKVPVLFEAPLAAGLLGAFVQAVSGGALYRKTSFLVDSLGKEVFAPHVQIVEDPHVP
CCCCCCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
RAMGSAPFDEEGVRTRARSVVRDGVVEGYFLSTYSARKLGTQTTGNAGGSHNIALRSALT
HHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHCCCCCCCCCCCCCCEEEEECCC
APGDDFDAMLKKLGTGLLLTELMGQGVNYVTGDYSRGAAGFWVENGEIQYPVEEITVAST
CCCCCHHHHHHHHCCHHHHHHHHHCCCCEEECCCCCCCCEEEEECCEEECCHHHHHHHHH
LQEMFRHIVAIGADSIVRGTKETGSVLIEQMTIAGQ
HHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]