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The map label for this gene is lepA [H]

Identifier: 237813197

GI number: 237813197

Start: 2885496

End: 2887289

Strand: Reverse

Name: lepA [H]

Synonym: GBP346_A2962

Alternate gene names: 237813197

Gene position: 2887289-2885496 (Counterclockwise)

Preceding gene: 237813198

Following gene: 237813196

Centisome position: 70.45

GC content: 63.15

Gene sequence:

>1794_bases
ATGGATCATATTCGCAATTTCTCGATCATCGCGCACATCGACCACGGCAAGTCGACGCTCGCGGATCGCATCATCCAGCT
TTGCGGCGGCCTGTCCGACCGGGAGATGGAATCGCAGGTGCTCGACTCGATGGACCTCGAGCGTGAGCGCGGCATCACGA
TCAAGGCGCAGACCGCCGCGCTCACCTATCGCGCGCGCGACGGCAAGGTCTACAACCTGAATCTCATCGATACCCCGGGG
CACGTCGATTTCTCGTACGAAGTGAGCCGCTCGCTGTCCGCGTGCGAGGGCGCGCTGCTCGTCGTCGACGCAAGCCAGGG
CGTCGAGGCGCAGACGGTCGCGAACTGCTATACGGCGATCGAGCTCGGCGTCGAGGTGGTGCCCGTCCTCAACAAGATCG
ATCTGCCGGCGGCGAACCCGGAGAACGCGATCGCCGAGATCGAGGACGTGATCGGCATCGACGCGATGGACGCGGTGCGC
TGCAGCGCGAAGACGGGCCTCGGCGTCGAGGACGTGCTCGAATCGCTGATCGCCAAGGTGCCGCCGCCGAAGGGCGATCC
CGATGCGCCGCTGCAGGCGCTCATCATCGATTCGTGGTTCGACAACTACGTCGGCGTCGTGATGCTCGTGCGCATCGTCA
ACGGCACGCTGCGCCCGAAGGAAAGGATCAAGCTGATGGCGACCGATGCGCAGTATGCGGTCGAGCACGTCGGCGTGTTC
ACGCCGAAGTCGCGCAATCTCGAATCGCTGTCTGCGGGGCAGGTGGGCTTCATCATCTCCGGCATCAAGGAACTGACGGC
CGCGAAGGTCGGCGATACGGTCACGCATGCGACGAAGCCCGCGCCCGAGCCGCTGCCGGGCTTCAAGGAAGTGAAGCCGC
AGGTGTTCGCGGGCCTCTATCCGGTCGAGGCGAACCAGTACGACGCGCTGCGCGAATCGCTCGAGAAGCTCAAGCTCAAC
GACGCGTCGCTGCAGTACGAGCCCGAGGTGTCGCAGGCGCTCGGCTTCGGCTTCCGCTGCGGCTTCCTCGGCCTGCTGCA
CATGGAGATCGTGCAGGAGCGGCTCGAACGCGAGTTCGACATGGACCTCATCACGACCGCGCCGACGGTCGTCTACGAGG
TCGTGCAAAGCGACGGCACGACGATCATGGTCGAGAACCCGGCGAAGATGCCGGAGCCCGCGCGGATCGCCGAGATTCGC
GAGCCGATCGTGACGGTCAACCTGTACATGCCGCAGGATTACGTCGGCTCGGTGATCACGCTGTGCGAGCAAAAGCGCGG
CACGCAGATCAACATGCAGTACCACGGCCGGCAGGTGCAGCTGACCTACGAGATCCCGATGGCCGAAATCGTGCTCGATT
TCTTCGATCGGCTGAAATCGGTGTCGCGCGGCTACGCGTCGATGGATTACGAGTTCAAGGAATACCGGACGTCCGATGTC
GTGAAGGTCGACATGCTGATCAACGGCGACAAGGTGGATGCGCTGTCGATCATCGTGCACCGTTCGCAGTCGCAGTATCG
CGGCCGCGAAGTCGCGGCGAAGATGCGCGAGATCATTCCGCGCCAGATGTACGACGTCGCGATCCAGGCGGCGATCGGCG
CGCACATCATCGCGCGCGAGAACATCAAGGCGCTGCGCAAGAACGTGCTGGCGAAGTGCTACGGCGGCGACATCACGCGC
AAGAAGAAACTGCTCGAAAAGCAGAAGGAAGGCAAGAAACGAATGAAGCAGGTGGGTTCGGTCGAGATCCCGCAGGAGGC
GTTCCTGGCGATCTTGCGAGTCGAAGACAAATAA

Upstream 100 bases:

>100_bases
CCTGGCGGGCCTTTTCGGCTAAAATAAGCCGTTTTTTCACCGACTTACAAGGCGTGCTCTGCGGTCGTCGAGCGCGCCTT
TTTCGCTTGATCGGCACTGA

Downstream 100 bases:

>100_bases
CAGGACTGATCCTCTATGAATTTTGCGTTGATCCTTTTCGTGCTCGTCGTCGTGACGGGCATTGCGTGGGTGTTGGACAA
GCTGGTTTTCCTGCCGCGGC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMESQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAMDAVR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKERIKLMATDAQYAVEHVGVF
TPKSRNLESLSAGQVGFIISGIKELTAAKVGDTVTHATKPAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIAEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGTQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRTSDV
VKVDMLINGDKVDALSIIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Sequences:

>Translated_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMESQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAMDAVR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKERIKLMATDAQYAVEHVGVF
TPKSRNLESLSAGQVGFIISGIKELTAAKVGDTVTHATKPAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIAEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGTQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRTSDV
VKVDMLINGDKVDALSIIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
>Mature_597_residues
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMESQVLDSMDLERERGITIKAQTAALTYRARDGKVYNLNLIDTPG
HVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAIELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAMDAVR
CSAKTGLGVEDVLESLIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKERIKLMATDAQYAVEHVGVF
TPKSRNLESLSAGQVGFIISGIKELTAAKVGDTVTHATKPAPEPLPGFKEVKPQVFAGLYPVEANQYDALRESLEKLKLN
DASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIAEIR
EPIVTVNLYMPQDYVGSVITLCEQKRGTQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRTSDV
VKVDMLINGDKVDALSIIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARENIKALRKNVLAKCYGGDITR
KKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=604, Percent_Identity=49.0066225165563, Blast_Score=617, Evalue=1e-177,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=38.75, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=46.3087248322148, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=46.3087248322148, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=46.3087248322148, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=43.6363636363636, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=43.6363636363636, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=43.6363636363636, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI53729339, Length=218, Percent_Identity=30.2752293577982, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=218, Percent_Identity=30.2752293577982, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=69.8653198653199, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=512, Percent_Identity=28.90625, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=36.1290322580645, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1789559, Length=225, Percent_Identity=28.4444444444444, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=39.3790849673203, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=26.3498920086393, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17533571, Length=161, Percent_Identity=38.5093167701863, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=230, Percent_Identity=31.7391304347826, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=38.8059701492537, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=38.8059701492537, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=33.8345864661654, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32566303, Length=247, Percent_Identity=28.7449392712551, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=223, Percent_Identity=29.5964125560538, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=47.5083056478405, Blast_Score=576, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6323098, Length=160, Percent_Identity=39.375, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=41.6666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=41.7391304347826, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=38.8888888888889, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=282, Percent_Identity=25.886524822695, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=156, Percent_Identity=31.4102564102564, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=46.0132890365449, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=37.888198757764, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.8378378378378, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.8378378378378, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.8378378378378, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI28574573, Length=137, Percent_Identity=43.7956204379562, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=39.5973154362416, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=34.5864661654135, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24652838, Length=172, Percent_Identity=33.1395348837209, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137572, Length=172, Percent_Identity=33.1395348837209, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45553807, Length=172, Percent_Identity=33.1395348837209, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45553816, Length=172, Percent_Identity=33.1395348837209, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24651721, Length=172, Percent_Identity=33.1395348837209, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864154, Length=172, Percent_Identity=33.1395348837209, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28572034, Length=219, Percent_Identity=29.2237442922374, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66222; Mature: 66222

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMESQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCEEEEEEEE
LTYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAMDAVRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
PPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKERIKLMATDAQYAVEHVGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCHHHHHHHHCCC
TPKSRNLESLSAGQVGFIISGIKELTAAKVGDTVTHATKPAPEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIAEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHH
LCEQKRGTQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRTSDV
HHHHCCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSIIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARE
EEEEEEECCCCCCCEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC
>Mature Secondary Structure
MDHIRNFSIIAHIDHGKSTLADRIIQLCGGLSDREMESQVLDSMDLERERGITIKAQTAA
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHCCCEEEEEEEE
LTYRARDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAI
EEEEECCCEEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
ELGVEVVPVLNKIDLPAANPENAIAEIEDVIGIDAMDAVRCSAKTGLGVEDVLESLIAKV
HHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHC
PPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKERIKLMATDAQYAVEHVGVF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCHHHHHHHHCCC
TPKSRNLESLSAGQVGFIISGIKELTAAKVGDTVTHATKPAPEPLPGFKEVKPQVFAGLY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCHHHCCHHHHHCCC
PVEANQYDALRESLEKLKLNDASLQYEPEVSQALGFGFRCGFLGLLHMEIVQERLEREFD
CCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
MDLITTAPTVVYEVVQSDGTTIMVENPAKMPEPARIAEIREPIVTVNLYMPQDYVGSVIT
CHHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEEECCHHHHHHHHH
LCEQKRGTQINMQYHGRQVQLTYEIPMAEIVLDFFDRLKSVSRGYASMDYEFKEYRTSDV
HHHHCCCCEEEEEECCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCE
VKVDMLINGDKVDALSIIVHRSQSQYRGREVAAKMREIIPRQMYDVAIQAAIGAHIIARE
EEEEEEECCCCCCCEEHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIKALRKNVLAKCYGGDITRKKKLLEKQKEGKKRMKQVGSVEIPQEAFLAILRVEDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA