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The map label for this gene is ntrC [H]

Identifier: 237813051

GI number: 237813051

Start: 2745983

End: 2747518

Strand: Reverse

Name: ntrC [H]

Synonym: GBP346_A2812

Alternate gene names: 237813051

Gene position: 2747518-2745983 (Counterclockwise)

Preceding gene: 237813052

Following gene: 237813043

Centisome position: 67.04

GC content: 70.38

Gene sequence:

>1536_bases
ATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCTGGGTGCTCGAGAAGGCGCTCGCGCGCGAGAGCTTCGC
GACGCGGAGCTTCGCGAACGTGCGCGACGCGCTCGCCGCGCTCGACCAGGAAACGCCGCAGGTGCTCGTGTCCGACATCC
GGATGCCGGGCGGCTCGGGGCTCGAGCTGCTGCAGGCGATGCACGACCGGCTGCCGGGGCTGCCCGTCATCATCATGACG
GCGTTCTCGGATCTGGACAGCGCGGTGGCGGCGTTTCAGGGCGGCGCGTTCGAATATCTCGCGAAGCCGTTCGACGTCGA
CAAGGCGGTCGAGCTGATCCGGCGCGCGGTCGAAGAGAGCCTGCGCGGCGGCGTGCCCGACGACGACAAGCTCGCCGAGG
CGCCCGAGATGCTCGGCCAGGCGCCCGCGATGCAGGACATGTTCCGCGCGATCGGCCGGCTGTCGCATTCGGCCGCGACG
GTGCTGATCACGGGCGAATCGGGCACCGGCAAGGAGCTCGTCGCGCGCGCGCTGCACCGGCACAGCCCACGCGCGAACGG
CCCGTTCATCGCGCTGAACACGGCGGCGATCCCGAAGGATCTGCTCGAATCCGAGCTGTTCGGCCACGAGCGCGGCGCGT
TCACGGGCGCGCAGACGACGCGCCAGGGCCGCTTCGAGCAGGCCGAGAACGGCACGCTGTTTCTCGACGAGATCGGCGAC
ATGCCGTTCGATCTGCAAACGCGCCTGTTGCGCGTGTTGTCGGACGGGCAGTTCTATCGCGTGGGCGGCCACAGCCCGCT
GCGCGCGAACGTGCGGGTGATCGCGGCGACGCATCAGAATCTCGAGGCGCGCGTGCGGCAGGGGCTGTTCCGCGAGGATC
TGTATCACCGGCTCAACGTGATCCGGCTGCGGCTGCCGCCGCTGCGCGAGCGCAGCGAGGACATCGCGCTGCTCACGCGT
CACTTCCTGCAGAAGAGCGCGCGCGACCTCGGCGTCGAGCCGAAGCGCGTGTCCGACGAGGCGCTCGCCTATCTGACGTC
GCTGCCGTTTCCGGGCAACGTCCGGCAGCTCGAGAATCTCGCGAACTGGCTGACGGTGATGGCGCCCGCGCAGACGGTTG
AGATCAAGGATCTGCCGCCCGATCTCGTCGCGACGCAGACGGCGTCCGGCAGCATGATGCCCGTCGAGGCCACGGTGGAG
GCGGATGCGCAGGCATCCGGCTTGGGCGTCGGCGCGGCGGAACCGCACGCGCCGAGCGCGGTCGCGGCCGTGCCGGCGAC
GGCGGGCGCGCCCACTGCGGTCGGCGCGCCCGCGGGGCACCCGCTGTGGGAGCACGGGTTGCGCACCGAGGTCGCACGCT
TGCTGCGCGAGAACTCGGCGGACGTGATGGACGCGCTCGCGCGCCGCTTCGAGGCCGCCGTGATTCGCGAGGCGCTCGAT
TTCACGCGCGGCCGCAAGGTCGAGGCGGCGGAGCGGCTCGGCATCGGCCGCAACACGATCACGCGCAAGATTCAGGAGCT
GCATCTCGAATCGTGA

Upstream 100 bases:

>100_bases
ATCGAAGTGGACAGCCGGCCGGGCCGGACCGAATTTCAGATTTTGCTGCCGCTCGACCATTGAGCGGCGGCGCAGCGAAT
CTCCATACCTCTGACCGACT

Downstream 100 bases:

>100_bases
TCGGCGCGGTGCGCCGGCGGCCATGCACGCGATGCCGCGCGTGGCGCGGCGAATGCAAGACGGGGCGCTTCGGCGCCCCG
TTTGCTTTGGATCGCGAAGC

Product: nitrogen regulation protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKPIWIVDDDQSIRWVLEKALARESFATRSFANVRDALAALDQETPQVLVSDIRMPGGSGLELLQAMHDRLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGVPDDDKLAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVATQTASGSMMPVEATVE
ADAQASGLGVGAAEPHAPSAVAAVPATAGAPTAVGAPAGHPLWEHGLRTEVARLLRENSADVMDALARRFEAAVIREALD
FTRGRKVEAAERLGIGRNTITRKIQELHLES

Sequences:

>Translated_511_residues
MKPIWIVDDDQSIRWVLEKALARESFATRSFANVRDALAALDQETPQVLVSDIRMPGGSGLELLQAMHDRLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGVPDDDKLAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVATQTASGSMMPVEATVE
ADAQASGLGVGAAEPHAPSAVAAVPATAGAPTAVGAPAGHPLWEHGLRTEVARLLRENSADVMDALARRFEAAVIREALD
FTRGRKVEAAERLGIGRNTITRKIQELHLES
>Mature_511_residues
MKPIWIVDDDQSIRWVLEKALARESFATRSFANVRDALAALDQETPQVLVSDIRMPGGSGLELLQAMHDRLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGVPDDDKLAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVATQTASGSMMPVEATVE
ADAQASGLGVGAAEPHAPSAVAAVPATAGAPTAVGAPAGHPLWEHGLRTEVARLLRENSADVMDALARRFEAAVIREALD
FTRGRKVEAAERLGIGRNTITRKIQELHLES

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=508, Percent_Identity=55.1181102362205, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1788550, Length=508, Percent_Identity=38.9763779527559, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1790437, Length=402, Percent_Identity=40.7960199004975, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788905, Length=401, Percent_Identity=39.9002493765586, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=53.3333333333333, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1789233, Length=258, Percent_Identity=46.1240310077519, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1789087, Length=272, Percent_Identity=45.5882352941176, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.936170212766, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=261, Percent_Identity=46.7432950191571, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1787583, Length=224, Percent_Identity=45.9821428571429, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=46.2555066079295, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=36.283185840708, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87081858, Length=300, Percent_Identity=30.6666666666667, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789809, Length=111, Percent_Identity=30.6306306306306, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55658; Mature: 55658

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESFATRSFANVRDALAALDQETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
LELLQAMHDRLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGVPDDDKLAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVATQTASGSMMPVEATVEADAQASGLGVGAAEPHAPSA
HHHHHHHCCCCEEEECCCCCHHHEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCH
VAAVPATAGAPTAVGAPAGHPLWEHGLRTEVARLLRENSADVMDALARRFEAAVIREALD
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FTRGRKVEAAERLGIGRNTITRKIQELHLES
HHCCCCCHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARESFATRSFANVRDALAALDQETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
LELLQAMHDRLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGVPDDDKLAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHSPLRANVRVIAATHQNLEARVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDEALAYLTSLPFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVATQTASGSMMPVEATVEADAQASGLGVGAAEPHAPSA
HHHHHHHCCCCEEEECCCCCHHHEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCH
VAAVPATAGAPTAVGAPAGHPLWEHGLRTEVARLLRENSADVMDALARRFEAAVIREALD
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
FTRGRKVEAAERLGIGRNTITRKIQELHLES
HHCCCCCHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]