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The map label for this gene is alkK [H]

Identifier: 237812655

GI number: 237812655

Start: 2358471

End: 2360111

Strand: Reverse

Name: alkK [H]

Synonym: GBP346_A2405

Alternate gene names: 237812655

Gene position: 2360111-2358471 (Counterclockwise)

Preceding gene: 237812661

Following gene: 237812654

Centisome position: 57.58

GC content: 67.89

Gene sequence:

>1641_bases
ATGGGTAAGCCGTTGCTGGGCCAGATGATGGATATGCCGCTGCTCGTGTCGTCGCTGATCGCGCATGCGGCGCGACACGC
GGGTGACGTCGAGATCGTGTCCCGGCGCGTCGAGGGCGACATTCACCGCTATACCTATCGCGATTGCGAAGCGCGTTCCA
AACGGCTCGCGCAGGCGCTGACCGGCCTGGGCGTGGGCGTGGGCGAGCGCATTGGAACGCTCGCGTGGAACGGCTACCGG
CACGTCGAGGCGTATTACGGCATCAGCGGGATGGGCGCCGTCTGCCATACGATCAACCCGCGCCTCTTTCCCGAGCAGAT
CGCGTACATCGTCAACCACGCCGAGGATCGATACGTGCTGTTCGACCTCACCTTCGCGCCGCTCGTCGATCAGCTCGCGC
CGCAATGCCCGAACGTGAAGGGCTGGATCGCGATGACGGACGATGCGCATCTGCCGAAGGGCGCGACGCCTTATCTCTGT
TACGAGACGCTCGTCGGCGCGCAGGACGGCGATTACGCCTGGCCGCTGCTCGACGAGCGGCAGGCGTCGTCGCTCTGCTA
CACGTCCGGCACGACGGGCCACCCGAAGGGCGCGCTCTATTCGCACCGCTCGACGGTGCTGCACGCGTACGGCGCGGCGC
TGCCCGACGCGATGGGGCTGTCCTCGCGCGACGCGGCGCTGCCCGTCGTGCCGATGTTCCACGTCAACGCATGGGGGCTG
CCGTACACGGCGGCGCTCACCGGCACGAAGCTCGTGCTGCCCGGCAAGGACCTCGACGGCAAATCGCTGTACGAGCTGAT
CGAAAGCGAGCGCGTGACGTTCTCGGCGGGCGTGCCCACCGTCTGGCTCGGTCTGCTCGGGTACATGCGCGAGGCCGGCG
TGCGCTTCTCGACGCTCGAGCGCACGGTGATCGGCGGCTCCGCGTGCCCGCCTTCGATGCTCGAGACGTTCGAGGACGTC
TACGACGTGCGTGTGATCCATGCATGGGGGATGACCGAGCTGTCGCCGCTCGGCACGCTGGCCAAGCTCAATTGGGCGCA
GTCGCAGCGCGGCATCGGCGAGCAGCGGCGGCTCCTCGAGAAGCAGGGCCGGGTGATCTACGGGATCGACATGCGCATCG
TCGGCGAGGACGGCCGCGAGCTGCCGTGGGACGGCGTCGCGTTCGGCGACTTGCAGGTGCGCGGGCCGTGGGTGATCGAC
CGCTATTTCGGAATCGACGCGTCGCCGCTCGTCGACGGCTGGTTCCCGACGGGCGACGTCGCGACGATCGACGCCGACGG
CTTCCTGCAGATCACAGATCGCAGCAAGGACGTGATCAAGTCGGGCGGCGAATGGATCAGCTCGATCGACGTCGAGAACG
TCGCGGTGGCGCACCCGGCCGTCGCGGAGGCCGCGTGCATCGCATGCGCGCATCCGAAATGGACCGAGCGGCCGCTCCTC
GTCGTCGTCAAGCGCGCCGGCATGGACGTGACGCGCGACGAACTGCTCGCGTTCTACGAGGGCAAGGTCGCGAAATGGTG
GATTCCGGACGATGTCGCGTTCGTCGACGCGCTGCCGCACACCGCGACGGGCAAGCTGCAAAAGCTCAAGCTGCGCGAGC
AATTCCGGGGCCACGTGCTGCCGACGGCCGTGGACGCGTAG

Upstream 100 bases:

>100_bases
TGTAAGTCCGCGCCGCGCGGGGCGCTCGAAGCCGTCCGCGCGCCGCCGAGCCGTTTGCAGCAGACCGTATCGGACAAATA
CTTCAGGGAGAAGGAAGACG

Downstream 100 bases:

>100_bases
CCCGCGCATCCGGCGCGTTGGCCGCGCGCGGGGGGCATGCCGACGCGCCGACGCGCGGGCGCGCGCGTCCGCGCGAATGG
CCGCCGTACGGGTCGCCACC

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQALTGLGVGVGERIGTLAWNGYR
HVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPKGATPYLC
YETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKGALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGL
PYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAGVPTVWLGLLGYMREAGVRFSTLERTVIGGSACPPSMLETFEDV
YDVRVIHAWGMTELSPLGTLAKLNWAQSQRGIGEQRRLLEKQGRVIYGIDMRIVGEDGRELPWDGVAFGDLQVRGPWVID
RYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLL
VVVKRAGMDVTRDELLAFYEGKVAKWWIPDDVAFVDALPHTATGKLQKLKLREQFRGHVLPTAVDA

Sequences:

>Translated_546_residues
MGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQALTGLGVGVGERIGTLAWNGYR
HVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPKGATPYLC
YETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKGALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGL
PYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAGVPTVWLGLLGYMREAGVRFSTLERTVIGGSACPPSMLETFEDV
YDVRVIHAWGMTELSPLGTLAKLNWAQSQRGIGEQRRLLEKQGRVIYGIDMRIVGEDGRELPWDGVAFGDLQVRGPWVID
RYFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLL
VVVKRAGMDVTRDELLAFYEGKVAKWWIPDDVAFVDALPHTATGKLQKLKLREQFRGHVLPTAVDA
>Mature_545_residues
GKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQALTGLGVGVGERIGTLAWNGYRH
VEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVLFDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPKGATPYLCY
ETLVGAQDGDYAWPLLDERQASSLCYTSGTTGHPKGALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGLP
YTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAGVPTVWLGLLGYMREAGVRFSTLERTVIGGSACPPSMLETFEDVY
DVRVIHAWGMTELSPLGTLAKLNWAQSQRGIGEQRRLLEKQGRVIYGIDMRIVGEDGRELPWDGVAFGDLQVRGPWVIDR
YFGIDASPLVDGWFPTGDVATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLLV
VVKRAGMDVTRDELLAFYEGKVAKWWIPDDVAFVDALPHTATGKLQKLKLREQFRGHVLPTAVDA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=531, Percent_Identity=26.7419962335217, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI187761345, Length=537, Percent_Identity=25.8845437616387, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI187761343, Length=537, Percent_Identity=25.8845437616387, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI28416953, Length=569, Percent_Identity=26.0105448154657, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI115511026, Length=518, Percent_Identity=22.007722007722, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI38505220, Length=455, Percent_Identity=24.1758241758242, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=23.4750462107209, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI157311624, Length=542, Percent_Identity=23.0627306273063, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=542, Percent_Identity=23.0627306273063, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI122937307, Length=516, Percent_Identity=22.4806201550388, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI42544132, Length=458, Percent_Identity=22.0524017467249, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI221142682, Length=515, Percent_Identity=24.4660194174757, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788107, Length=531, Percent_Identity=25.6120527306968, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI145693145, Length=520, Percent_Identity=25, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=26.3888888888889, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1786810, Length=356, Percent_Identity=25.8426966292135, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994703, Length=561, Percent_Identity=23.8859180035651, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=561, Percent_Identity=23.8859180035651, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=561, Percent_Identity=23.8859180035651, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=518, Percent_Identity=26.2548262548263, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=530, Percent_Identity=25.0943396226415, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=368, Percent_Identity=27.445652173913, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=539, Percent_Identity=23.0055658627087, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=24.3589743589744, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=527, Percent_Identity=25.0474383301708, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=22.8070175438596, Blast_Score=105, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=22.5490196078431, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=360, Percent_Identity=24.1666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71996755, Length=360, Percent_Identity=24.4444444444444, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24581924, Length=543, Percent_Identity=26.8876611418048, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859661, Length=477, Percent_Identity=27.2536687631027, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=23.6842105263158, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19922652, Length=506, Percent_Identity=21.7391304347826, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648260, Length=509, Percent_Identity=22.5933202357564, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653035, Length=502, Percent_Identity=20.3187250996016, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356441, Length=369, Percent_Identity=22.4932249322493, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=550, Percent_Identity=20.3636363636364, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281365686, Length=359, Percent_Identity=21.4484679665738, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648255, Length=562, Percent_Identity=19.5729537366548, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI161076582, Length=510, Percent_Identity=20.7843137254902, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648257, Length=482, Percent_Identity=21.9917012448133, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356947, Length=497, Percent_Identity=21.9315895372233, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59811; Mature: 59680

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TGLGVGVGERIGTLAWNGYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVL
HHCCCCHHHHHHHEEECCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEE
FDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPKGATPYLCYETLVGAQDGDYAWPLLDER
EEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCC
QASSLCYTSGTTGHPKGALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGL
CCCCEEEECCCCCCCCCCEECCHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCCC
PYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAGVPTVWLGLLGYMREAGVRFSTLE
CEEEEECCCEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHH
RTVIGGSACPPSMLETFEDVYDVRVIHAWGMTELSPLGTLAKLNWAQSQRGIGEQRRLLE
HHEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHCCHHHHHCCCCHHHHHHH
KQGRVIYGIDMRIVGEDGRELPWDGVAFGDLQVRGPWVIDRYFGIDASPLVDGWFPTGDV
HCCCEEEEEEEEEECCCCCCCCCCCEEECCEEECCCEEEEEECCCCCCCCCCCCCCCCCE
ATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLL
EEECCCCEEEEECCCHHHHHCCCHHHHHCCCCCEEECCCHHHHHHHHEECCCCCCCCCEE
VVVKRAGMDVTRDELLAFYEGKVAKWWIPDDVAFVDALPHTATGKLQKLKLREQFRGHVL
EEEECCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCC
PTAVDA
CCCCCC
>Mature Secondary Structure 
GKPLLGQMMDMPLLVSSLIAHAARHAGDVEIVSRRVEGDIHRYTYRDCEARSKRLAQAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
TGLGVGVGERIGTLAWNGYRHVEAYYGISGMGAVCHTINPRLFPEQIAYIVNHAEDRYVL
HHCCCCHHHHHHHEEECCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCEEE
FDLTFAPLVDQLAPQCPNVKGWIAMTDDAHLPKGATPYLCYETLVGAQDGDYAWPLLDER
EEECHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCC
QASSLCYTSGTTGHPKGALYSHRSTVLHAYGAALPDAMGLSSRDAALPVVPMFHVNAWGL
CCCCEEEECCCCCCCCCCEECCHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEEEECCCCC
PYTAALTGTKLVLPGKDLDGKSLYELIESERVTFSAGVPTVWLGLLGYMREAGVRFSTLE
CEEEEECCCEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHH
RTVIGGSACPPSMLETFEDVYDVRVIHAWGMTELSPLGTLAKLNWAQSQRGIGEQRRLLE
HHEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHCCHHHHHCCCCHHHHHHH
KQGRVIYGIDMRIVGEDGRELPWDGVAFGDLQVRGPWVIDRYFGIDASPLVDGWFPTGDV
HCCCEEEEEEEEEECCCCCCCCCCCEEECCEEECCCEEEEEECCCCCCCCCCCCCCCCCE
ATIDADGFLQITDRSKDVIKSGGEWISSIDVENVAVAHPAVAEAACIACAHPKWTERPLL
EEECCCCEEEEECCCHHHHHCCCHHHHHCCCCCEEECCCHHHHHHHHEECCCCCCCCCEE
VVVKRAGMDVTRDELLAFYEGKVAKWWIPDDVAFVDALPHTATGKLQKLKLREQFRGHVL
EEEECCCCCCCHHHHHHHHCCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCC
PTAVDA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]