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The map label for this gene is rimO [H]

Identifier: 237812521

GI number: 237812521

Start: 2235124

End: 2236515

Strand: Reverse

Name: rimO [H]

Synonym: GBP346_A2270

Alternate gene names: 237812521

Gene position: 2236515-2235124 (Counterclockwise)

Preceding gene: 237812522

Following gene: 237812520

Centisome position: 54.57

GC content: 66.02

Gene sequence:

>1392_bases
TTGGAAAATTCATTGAAATCAAGCGGTAAGCCGCTCGCCGCGCCCAAGGTGGGTATGGTTTCGCTCGGGTGCCCGAAGGC
CCTCGTCGACTCCGAGCAGATCATCACGCAACTGCGCGCCGAAGGCTACGAAATCTCCGGCACCTATGACGGCGCGGATC
TCGTCGTCGTCAACACTTGCGGCTTCATCGACGAGGCGGTGCAGGAAAGCCTCGACGCGATCGGCGAAGCGCTCGCCGAA
AACGGCAAGGTGATCGTCACCGGCTGCCTCGGCGCGAAGAAGAGCGCGAGCGGCTCCGGCCTCATCGCGGAAGTGCATCC
GAAGGTGCTCGCCGTCACGGGCCCGCATGCGGTCGGCGAAGTGATGCAGGCGGTGCACAGCCATCTGCCGAAGCCGCACG
ATCCGTTCGTCGACCTCGTGCCGGCCGCGGGCATCAAGCTCACGCCGCGTCACTACGCGTATCTGAAGATCTCCGAAGGC
TGCAATCACCGCTGCTCGTTCTGCATCATCCCGTCGATGCGCGGCGAGCTCGTGTCGCGACCCGTCGCCGAGGTGATGCT
CGAAGCCGAGAATCTCTTCAAGTCCGGCGTGAAGGAGCTGCTCGTGATCTCGCAGGACACGAGCGCGTACGGCGTCGACG
TCAAGTACCGCACGGGCTTCTGGAACGGCCGGCCGCTCAAGACGCGGATGACCGAGCTCGTCGGCGCGCTCGGCGAGCTC
GCCGCGCAGTACGGCGCGTGGGTGCGCCTGCACTACGTGTATCCGTATCCGCACGTCGACGAGATCATTCCGATGATGGC
GCAAGGCCCGCTCAAGGGCCATGTGCTGCCGTATCTCGACGTGCCGTTCCAGCACGCGCATCCGGAGGTGCTCAAGCGAA
TGAAGCGCCCGGCGAACGCGGAGCGGGTGCTCGAGCGCGTGCAGAAGTGGCGCGAGATCTGCCCGGATCTGACGATTCGC
AGCACGTTCATTGCCGGCTTTCCGGGCGAGACCGACGCGCAATTCGAGACACTCCTCGATTTCATCCGCGAGGCCGAGCT
CGATCGCGTCGGCTGCTTCGCGTATTCTCCGGTCGAGGGCGCGAGCGCGAACGCGCTCGACGGCGCGCTGCCCGACGACG
TGCGCGAGGCACGCCGCGCGCGCTTCATGGAAGTCGCCGAGGAGGTGTCCGCCGCGCGCATCGCGCGCAAGATCGGCAAG
ACGCTCAAGGTGTTGATCGACGAGGTCAACGCGGAAGGCGGCATCGGCCGCACCGCGGCCGATGCGCCCGAGATCGACGG
CGTCGTCTATGTCGAGCCGGCGGCCAAGGCGTCGAAGCGCTACAAGGTCGGCGAGTTCGTGTCCGTGAAGATCACGGGCG
CCGACGGCCACGATCTGTGGGGCGAGGTCTGA

Upstream 100 bases:

>100_bases
GCGCACCCTCGGAGAAACGCCGCCGCGCGGAGGGTAAAATGCCGGCAAACCCAAGGTGTGCCGCCCGCGGACGCCGTCCC
CTCATGCATGGAAGCCATAG

Downstream 100 bases:

>100_bases
CGATGCCGGCGACGTTTCCGGACGTGCTCGCGCTCGGCGAGGCGATGGTCGAATTCAACCAGGCGCGGCCGGGCGAGCCG
AATTATCTGCAGGGCTTCGG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MENSLKSSGKPLAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIAEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGELVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPEVLKRMKRPANAERVLERVQKWREICPDLTIR
STFIAGFPGETDAQFETLLDFIREAELDRVGCFAYSPVEGASANALDGALPDDVREARRARFMEVAEEVSAARIARKIGK
TLKVLIDEVNAEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGEFVSVKITGADGHDLWGEV

Sequences:

>Translated_463_residues
MENSLKSSGKPLAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIAEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGELVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPEVLKRMKRPANAERVLERVQKWREICPDLTIR
STFIAGFPGETDAQFETLLDFIREAELDRVGCFAYSPVEGASANALDGALPDDVREARRARFMEVAEEVSAARIARKIGK
TLKVLIDEVNAEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGEFVSVKITGADGHDLWGEV
>Mature_463_residues
MENSLKSSGKPLAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALAE
NGKVIVTGCLGAKKSASGSGLIAEVHPKVLAVTGPHAVGEVMQAVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEG
CNHRCSFCIIPSMRGELVSRPVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPEVLKRMKRPANAERVLERVQKWREICPDLTIR
STFIAGFPGETDAQFETLLDFIREAELDRVGCFAYSPVEGASANALDGALPDDVREARRARFMEVAEEVSAARIARKIGK
TLKVLIDEVNAEGGIGRTAADAPEIDGVVYVEPAAKASKRYKVGEFVSVKITGADGHDLWGEV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=429, Percent_Identity=27.7389277389277, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI28872784, Length=490, Percent_Identity=24.6938775510204, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28872782, Length=490, Percent_Identity=24.6938775510204, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787057, Length=448, Percent_Identity=62.7232142857143, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1786882, Length=462, Percent_Identity=26.1904761904762, Blast_Score=141, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=482, Percent_Identity=24.2738589211618, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71996771, Length=303, Percent_Identity=27.3927392739274, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=501, Percent_Identity=26.1477045908184, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=25.8620689655172, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 50163; Mature: 50163

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSLKSSGKPLAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALAENGKVIVTGCLGAKKSASGSGLIAEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCHHHHH
VMQAVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGELVSR
HHHHHHHHCCCCCCCCHHHHCCCCEEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHHC
PVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPEVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCH
ERVLERVQKWREICPDLTIRSTFIAGFPGETDAQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
ASANALDGALPDDVREARRARFMEVAEEVSAARIARKIGKTLKVLIDEVNAEGGIGRTAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DAPEIDGVVYVEPAAKASKRYKVGEFVSVKITGADGHDLWGEV
CCCCCCCEEEECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MENSLKSSGKPLAAPKVGMVSLGCPKALVDSEQIITQLRAEGYEISGTYDGADLVVVNTC
CCCCCCCCCCCCCCCCCCEEECCCCHHHHCHHHHHHHHHHCCEEEECCCCCCCEEEEECC
GFIDEAVQESLDAIGEALAENGKVIVTGCLGAKKSASGSGLIAEVHPKVLAVTGPHAVGE
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCHHHHH
VMQAVHSHLPKPHDPFVDLVPAAGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGELVSR
HHHHHHHHCCCCCCCCHHHHCCCCEEECCCEEEEEEECCCCCCCCCEEEECCHHHHHHHC
PVAEVMLEAENLFKSGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVGALGEL
HHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
AAQYGAWVRLHYVYPYPHVDEIIPMMAQGPLKGHVLPYLDVPFQHAHPEVLKRMKRPANA
HHHHCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCH
ERVLERVQKWREICPDLTIRSTFIAGFPGETDAQFETLLDFIREAELDRVGCFAYSPVEG
HHHHHHHHHHHHHCCCCEEEHHEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCC
ASANALDGALPDDVREARRARFMEVAEEVSAARIARKIGKTLKVLIDEVNAEGGIGRTAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DAPEIDGVVYVEPAAKASKRYKVGEFVSVKITGADGHDLWGEV
CCCCCCCEEEECCCHHHHCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA