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The map label for this gene is thlA [H]

Identifier: 237812519

GI number: 237812519

Start: 2232915

End: 2234198

Strand: Reverse

Name: thlA [H]

Synonym: GBP346_A2268

Alternate gene names: 237812519

Gene position: 2234198-2232915 (Counterclockwise)

Preceding gene: 237812520

Following gene: 237812518

Centisome position: 54.51

GC content: 69.78

Gene sequence:

>1284_bases
GTGAGCCCGCGCGCGCGGCGCGCGATCCGAATGCATGACGTGCGCGGCCGCGACCGCGCGCTCCATCGGTTTTCATCAAG
AGAAGAGAGGGAGGCAACGATGCAACGCGAAGTAGTGGTGGTGAGCGGCGTACGCACGGCGATCGGCGATTTCGGCGGCA
GCCTGAAGGACTTCGCGCCGACCGAGCTCGGCGCGCGGGTCGTGCGCGAGGTGCTGTCGCGCGCGCAGGTGTCGGGCGAC
GAGGTCGGCCACGTCGTGTTCGGCAATGTCGTGCACACGGAGCCGAAGGACATGTATCTCGCGCGCGTCGCGGCGATCAA
CGGCGGCGTCGCGCAGCACGCGCCCGCGCTGACGGTCAACCGTCTCTGCGGCTCCGGCCTGCAGGCGATCGTGTCGGCCG
CGCAGAACGTGCTGCTCGGCGACGCGGACATCGCGGTCGCGGGCGGCGCGGAGAACATGAGCCGCGCGCCGTACTCGGTG
CCGGCCGCGCGCTTCGGCCAGCGCATGGGCGATGCGAAGCTCGTCGACATGATGATCGGCGCGCTCAACGATCCGTTCCA
GTCGATTCACATGGGCGTGACGGCCGAGAACGTCGCGGCGAAGTACGGGATCACGCGCGACGCGCAGGACGCGCTCGCGC
TCGAATCCCACCGGCGCGCGTCGCATGCGACGAAATCCGGCTATTTCAAGGATCAGATCCTGCCGATCGAGATCGCGTCG
CGCAAGGGCACGGTCGTGTTCGACGCCGACGAGCACGTGCGCCACGACGCGTCGCTCGACGACTTCACGAAGCTCAAGCC
CGTGTTCGCCAAGGAGAACGGCACGGTGACGGCGGGCAACGCGTCGGGCATCAACGATGCGGCCGCCGCGGTGGTGCTGA
TGGAGCGCGGCGTCGCCGACAAGCGCGGCGCGAAGCCGCTCGCGCGCCTCGTCTCGTATGCGCACGCGGGCGTCGATCCG
GCATACATGGGCATCGGCCCCGTGCCCGCGACGAAGAAGGCGCTCGAGCGCGCGGGCATCACCGTCGCCGATCTCGACGT
GATCGAGGCGAACGAGGCGTTCGCCGCGCAGGCGTGCGCGGTGTCGAACGCGCTCGGTCTCGATCCGGCGAAGGTCAACC
CGAACGGCTCGGGCATCTCGCTCGGCCATCCGATCGGCGCGACGGGCGCGCTCATCACCGTGAAGGCGCTGTACGAGCTG
CAGCGGATCGGCGGGCGCTACGCGCTCGTCACGATGTGCATCGGCGGCGGGCAGGGCATTGCCGCGGTGTTCGAGCGTCT
GTGA

Upstream 100 bases:

>100_bases
CCGCACGCTATGCGAACGCGGCGGCCGCGCTATCGACGCAAGGGTTCGGCGCGGTCGCGCCGATCCCGTCGCGCGACGCG
GTCGAGCGGCTATTGGGCGC

Downstream 100 bases:

>100_bases
CGGGAGGGCCGCGCAGTTGAAGCTGATCACCGCAATCCGCGGCGCGGCGCTCGCCGCCGCGCTGGCGACGGCGGCGGGGC
TCGCGTTCGCACAGTCCTCC

Product: beta-ketothiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSPRARRAIRMHDVRGRDRALHRFSSREEREATMQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGD
EVGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVNRLCGSGLQAIVSAAQNVLLGDADIAVAGGAENMSRAPYSV
PAARFGQRMGDAKLVDMMIGALNDPFQSIHMGVTAENVAAKYGITRDAQDALALESHRRASHATKSGYFKDQILPIEIAS
RKGTVVFDADEHVRHDASLDDFTKLKPVFAKENGTVTAGNASGINDAAAAVVLMERGVADKRGAKPLARLVSYAHAGVDP
AYMGIGPVPATKKALERAGITVADLDVIEANEAFAAQACAVSNALGLDPAKVNPNGSGISLGHPIGATGALITVKALYEL
QRIGGRYALVTMCIGGGQGIAAVFERL

Sequences:

>Translated_427_residues
MSPRARRAIRMHDVRGRDRALHRFSSREEREATMQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGD
EVGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVNRLCGSGLQAIVSAAQNVLLGDADIAVAGGAENMSRAPYSV
PAARFGQRMGDAKLVDMMIGALNDPFQSIHMGVTAENVAAKYGITRDAQDALALESHRRASHATKSGYFKDQILPIEIAS
RKGTVVFDADEHVRHDASLDDFTKLKPVFAKENGTVTAGNASGINDAAAAVVLMERGVADKRGAKPLARLVSYAHAGVDP
AYMGIGPVPATKKALERAGITVADLDVIEANEAFAAQACAVSNALGLDPAKVNPNGSGISLGHPIGATGALITVKALYEL
QRIGGRYALVTMCIGGGQGIAAVFERL
>Mature_426_residues
SPRARRAIRMHDVRGRDRALHRFSSREEREATMQREVVVVSGVRTAIGDFGGSLKDFAPTELGARVVREVLSRAQVSGDE
VGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVNRLCGSGLQAIVSAAQNVLLGDADIAVAGGAENMSRAPYSVP
AARFGQRMGDAKLVDMMIGALNDPFQSIHMGVTAENVAAKYGITRDAQDALALESHRRASHATKSGYFKDQILPIEIASR
KGTVVFDADEHVRHDASLDDFTKLKPVFAKENGTVTAGNASGINDAAAAVVLMERGVADKRGAKPLARLVSYAHAGVDPA
YMGIGPVPATKKALERAGITVADLDVIEANEAFAAQACAVSNALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQ
RIGGRYALVTMCIGGGQGIAAVFERL

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=45.29262086514, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI148539872, Length=391, Percent_Identity=45.2685421994885, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=41.6666666666667, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4557237, Length=408, Percent_Identity=38.4803921568627, Blast_Score=262, Evalue=4e-70,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=35.2941176470588, Blast_Score=201, Evalue=9e-52,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=31.7135549872123, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1788554, Length=398, Percent_Identity=50, Blast_Score=347, Evalue=8e-97,
Organism=Escherichia coli, GI87082165, Length=392, Percent_Identity=46.6836734693878, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=43.3168316831683, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=39.4472361809045, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=35.4523227383863, Blast_Score=220, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI133906874, Length=391, Percent_Identity=45.7800511508951, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI25147385, Length=397, Percent_Identity=34.0050377833753, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=398, Percent_Identity=33.6683417085427, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=31.2056737588652, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17535917, Length=409, Percent_Identity=27.1393643031785, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6325229, Length=401, Percent_Identity=38.6533665835411, Blast_Score=258, Evalue=9e-70,
Organism=Saccharomyces cerevisiae, GI6322031, Length=442, Percent_Identity=35.0678733031674, Blast_Score=225, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655093, Length=392, Percent_Identity=46.9387755102041, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=43.1122448979592, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640423, Length=411, Percent_Identity=39.9026763990268, Blast_Score=272, Evalue=3e-73,
Organism=Drosophila melanogaster, GI17137578, Length=443, Percent_Identity=30.9255079006772, Blast_Score=181, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 44829; Mature: 44697

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRARRAIRMHDVRGRDRALHRFSSREEREATMQREVVVVSGVRTAIGDFGGSLKDFAP
CCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHHCCCCCHHCCH
TELGARVVREVLSRAQVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVN
HHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHCCCHHHCCCHHHHH
RLCGSGLQAIVSAAQNVLLGDADIAVAGGAENMSRAPYSVPAARFGQRMGDAKLVDMMIG
HHHCHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
ALNDPFQSIHMGVTAENVAAKYGITRDAQDALALESHRRASHATKSGYFKDQILPIEIAS
HCCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
RKGTVVFDADEHVRHDASLDDFTKLKPVFAKENGTVTAGNASGINDAAAAVVLMERGVAD
CCCCEEECCHHHHHCCCCCHHHHHCCCCEECCCCEEEECCCCCCCHHHHHHHHHHCCCCC
KRGAKPLARLVSYAHAGVDPAYMGIGPVPATKKALERAGITVADLDVIEANEAFAAQACA
CCCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCCCEEEECEEEECCHHHHHHHHH
VSNALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGI
HHHHCCCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHH
AAVFERL
HHHHHCC
>Mature Secondary Structure 
SPRARRAIRMHDVRGRDRALHRFSSREEREATMQREVVVVSGVRTAIGDFGGSLKDFAP
CCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCEEEEECHHHHHHHCCCCCHHCCH
TELGARVVREVLSRAQVSGDEVGHVVFGNVVHTEPKDMYLARVAAINGGVAQHAPALTVN
HHHHHHHHHHHHHHHHCCCCCCCEEEECCEEECCCHHHHHHHHHHHCCCHHHCCCHHHHH
RLCGSGLQAIVSAAQNVLLGDADIAVAGGAENMSRAPYSVPAARFGQRMGDAKLVDMMIG
HHHCHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHH
ALNDPFQSIHMGVTAENVAAKYGITRDAQDALALESHRRASHATKSGYFKDQILPIEIAS
HCCCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEC
RKGTVVFDADEHVRHDASLDDFTKLKPVFAKENGTVTAGNASGINDAAAAVVLMERGVAD
CCCCEEECCHHHHHCCCCCHHHHHCCCCEECCCCEEEECCCCCCCHHHHHHHHHHCCCCC
KRGAKPLARLVSYAHAGVDPAYMGIGPVPATKKALERAGITVADLDVIEANEAFAAQACA
CCCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCCCEEEECEEEECCHHHHHHHHH
VSNALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRIGGRYALVTMCIGGGQGI
HHHHCCCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHH
AAVFERL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]