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The map label for this gene is potH [H]

Identifier: 237812499

GI number: 237812499

Start: 2211187

End: 2212050

Strand: Reverse

Name: potH [H]

Synonym: GBP346_A2248

Alternate gene names: 237812499

Gene position: 2212050-2211187 (Counterclockwise)

Preceding gene: 237812500

Following gene: 237812498

Centisome position: 53.97

GC content: 62.5

Gene sequence:

>864_bases
GTGATCGCGGGCCCGTTCTTCTGGCTCGTGCTGTTCTTCCTCGTGCCGTTCGTGCTCGTCGTCAAGATCAGTTTCGCGCA
GTTGCAGCTCGGCATTCCGCCGTATACGGAACTCACGGCGCTCGCCGACGGCGTGCTCAGCATCAAGCTGAACCTGCAGC
ACTACGCGTTCCTGTTCAGCGACAGCCTGTACTTCGCGACCTACGTGAACTCGGTCGTCGTCGCGGCCGTCACGACGCTG
CTGTGCCTGCTGATCGGCTATCCGATGGCGTACTACATCGCGCGCTCGAACCCGGGCACGCGCAACCTGCTGATGATGGC
GGTGATGCTGCCGTTCTGGACGTCGTTCCTGATCCGCGTGTATGCATGGATCGGCATCCTGAAGAACAACGGGCTGCTCA
ACAACTTCCTGCTGTGGACGGGGCTCATCAGCACGCCGATCGAGCTGTACCGGACGAACGTCGGTGTCTATATCGGGATG
GTCTACTCGTATCTGCCGTTCCTCGTGATGCCGCTCTACGCGCACCTGGTGAAGATGGACCTGCGCCTGCTCGAAGCCGC
CTACGACCTGGGCGCGAAGCCGTGGAAGGCGTTCGTGCAGATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCGGGCT
GCCTGCTCGTGTTCATTCCGGCCGTCGGCGAGTACGTGATTCCCGAGCTGCTCGGCGGCGCGAACACGCTGATGATCGGC
CGCGTGATGTGGAACGAGTTCTTCAACAACGCGGACTGGCCGATGGCGTCGGCGGTGACCTGCGCGATGGTGCTGCTGCT
TCTCGTGCCGATGGCGATGTTCCAGCACTTCCAGGCGAAGGAGCAGGAGGGCCACCGCCGATGA

Upstream 100 bases:

>100_bases
GCTGGAGCGCCGCGGCCGGCGTGGTGCTGACGTCATGAACGCGCTTTCGTCCCTGTTTGCGTGGCCCGTCAGGAAGTTCG
GCCTCACAGGCAGAACGGCC

Downstream 100 bases:

>100_bases
AGCCGAATCGCTATCTGCAGGTGCTGATGCTCGCGATCGGCTTCGGTTTCCTCTATATCCCGATCGCGAGCCTCGTCGTC
TATTCGTTCAACGATTCGAA

Product: putrescine transport system permease protein PotH

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTL
LCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYIGM
VYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFIPAVGEYVIPELLGGANTLMIG
RVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR

Sequences:

>Translated_287_residues
MIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTL
LCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYIGM
VYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFIPAVGEYVIPELLGGANTLMIG
RVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR
>Mature_287_residues
MIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFLFSDSLYFATYVNSVVVAAVTTL
LCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRVYAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYIGM
VYSYLPFLVMPLYAHLVKMDLRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFIPAVGEYVIPELLGGANTLMIG
RVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=264, Percent_Identity=59.469696969697, Blast_Score=329, Evalue=1e-91,
Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=42.4812030075188, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1787713, Length=245, Percent_Identity=28.9795918367347, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32272; Mature: 32272

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFLFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCEEEEEEHHHHHHHHH
DSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRV
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFIPAVGEYVIPELLGGANTLMIG
HHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAGPFFWLVLFFLVPFVLVVKISFAQLQLGIPPYTELTALADGVLSIKLNLQHYAFLFS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHCCCEEEEEEHHHHHHHHH
DSLYFATYVNSVVVAAVTTLLCLLIGYPMAYYIARSNPGTRNLLMMAVMLPFWTSFLIRV
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YAWIGILKNNGLLNNFLLWTGLISTPIELYRTNVGVYIGMVYSYLPFLVMPLYAHLVKMD
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLLEAAYDLGAKPWKAFVQITLPLSKNGIIAGCLLVFIPAVGEYVIPELLGGANTLMIG
HHHHHHHHHCCCCCCEEHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RVMWNEFFNNADWPMASAVTCAMVLLLLVPMAMFQHFQAKEQEGHRR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]