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The map label for this gene is citA [H]

Identifier: 237812348

GI number: 237812348

Start: 2060798

End: 2062120

Strand: Direct

Name: citA [H]

Synonym: GBP346_A2096

Alternate gene names: 237812348

Gene position: 2060798-2062120 (Clockwise)

Preceding gene: 237812347

Following gene: 237812349

Centisome position: 50.28

GC content: 69.46

Gene sequence:

>1323_bases
GTGAAGCAATCCGCAGTTCCCCTCGACACGGGCACGGCCGTGTCGCCCCCTACGTCGAGCGCCGGGCGAGCAATTGCCGC
GGCGTCGATCGGCAACGCGCTCGAGTGGTACGACTTTTCCGTCTATGCATTCTTCGCCGTCTACATCGCGCGGAACTTCT
TCCATCGAGGCAACACCGGCACCGAGCTGGTCGAGGCTTTCATGGCGTTCGGCATCGGCTTCATCGCACGGCCGCTCGGC
GCGCTCGCGATCGGCGTATACGGCGACCGCGCGGGCCGCAAGGCCGCGCTCACGCTGACCATCCTCGTGATGGCAACCGG
CACGGGCGTCATCGCGTTCGCGCCGCCATACGCCGCGATCGGCGTGGGCGCGCCGCTGCTGATCCTCTGCGGGAGGCTAC
TGCAGGGCTTCTCGGCGGGCGGCGAAGTGGGCGGCGCGGCGGCGTTTCTCATCGAGCACGCGCCGGCGGACCGCAAGGGC
TGCTACGCGTCGTGTCTGCAGGCGAGCATGGCCGCGTCGAACATCCTCGGCGCGCTGGTCGCGACCGGCGTGACGCTTAC
GCTGACGCGCGAACAGATCGGCGATTGGGGATGGCGGATTCCGTTCATCCTCGGCCTCGCGATCGCGCCGGTCGGCCTCT
GGCTGCGCAGGACGCTCGACGAGACGCCGCACTTCCGCGCCGAGATGGCGCGCGCGCAGCACGCGCATGCGGAACAGAAA
GCGCCGCTTCTGCAGGTGGTGCGCGACCACCCGCGCGCGCTCGCCGTCGGCACGGGATTCTCGGTGCTCTGGGCCGTGTG
CGTCTACGCGCTGGTGATCTATATGCCGACGCACGCGCAGCGCGCACTGCATTTCGACGGGCGCGACGCGTTCATCGCGT
CGCTGGTCGGCAACTGCCTGATGGCCGTCACCTGCGTGTGCGCGGGAAGCTGGTCCGACCGCCTCGGCCGGCGCACGGTG
CTCGCCGCCGGCGCGGCGCTGATGCTCGTGTCGGTCTATCCGCTGCTGCGCTGGCTGAGCGACGTGCACACGCTCGCCGC
GCTCCTTACCGTCCAGAGCGCGTTCTGCGTGTTGGTGGCCATCTTCACGGGAGTGGCGCCCGCAGCGCTGTCGGAGCTGT
TCCCGACCCGCGTACGCGCGACCGGCATGTCCCTGTCCTACAACATCGCCACGACGATCTTCGGCGGCTTCGCGCCCGCG
ATCCTCGCATGGCTCACGCAACAGACCGGCAATCCGTTTGCGCCGGCCTGGTACGTGATGGTGGCGAGCGCCATCGCGCT
CGCATCGATCGCCGCGCTTTCTTCCACGCCACGCCACGCCTGA

Upstream 100 bases:

>100_bases
CGCCGGCTACGAAGAAGCGCTCGATGAAGCGCAACGCCGGCAAATTCCGCATTTCCGGCTGTAAAGGCCGCCCCGCACGA
CGACAGGACAGGAGATCGAA

Downstream 100 bases:

>100_bases
TTTCGGAGAACCGCTCCATGCAACGCTTCCTCACGCGGAGCGCGATCGCGCTCGCACTTTTCGCCGCCGTCCCGGCCTTC
GCGGACGTCACGCTCGACGG

Product: MFS transporter, metabolite:H+ symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKQSAVPLDTGTAVSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLG
ALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKG
CYASCLQASMAASNILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQK
APLLQVVRDHPRALAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTV
LAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPA
ILAWLTQQTGNPFAPAWYVMVASAIALASIAALSSTPRHA

Sequences:

>Translated_440_residues
MKQSAVPLDTGTAVSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLG
ALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKG
CYASCLQASMAASNILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQK
APLLQVVRDHPRALAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTV
LAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPA
ILAWLTQQTGNPFAPAWYVMVASAIALASIAALSSTPRHA
>Mature_440_residues
MKQSAVPLDTGTAVSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTGTELVEAFMAFGIGFIARPLG
ALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAIGVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKG
CYASCLQASMAASNILGALVATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQK
APLLQVVRDHPRALAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCLMAVTCVCAGSWSDRLGRRTV
LAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVAIFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPA
ILAWLTQQTGNPFAPAWYVMVASAIALASIAALSSTPRHA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=32.9383886255924, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788942, Length=426, Percent_Identity=35.6807511737089, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1788292, Length=442, Percent_Identity=29.8642533936652, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1789941, Length=423, Percent_Identity=33.096926713948, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87082231, Length=438, Percent_Identity=24.4292237442922, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082404, Length=403, Percent_Identity=22.3325062034739, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46192; Mature: 46192

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSAVPLDTGTAVSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTG
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
TELVEAFMAFGIGFIARPLGALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAI
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHEEEEEEECCCCEEEECCCHHHH
GVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAASNILGALV
HCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQK
HCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
APLLQVVRDHPRALAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCL
CHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHH
MAVTCVCAGSWSDRLGRRTVLAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVA
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGNPFAPAWYVM
HHHCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VASAIALASIAALSSTPRHA
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQSAVPLDTGTAVSPPTSSAGRAIAAASIGNALEWYDFSVYAFFAVYIARNFFHRGNTG
CCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
TELVEAFMAFGIGFIARPLGALAIGVYGDRAGRKAALTLTILVMATGTGVIAFAPPYAAI
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHHEEEEEEECCCCEEEECCCHHHH
GVGAPLLILCGRLLQGFSAGGEVGGAAAFLIEHAPADRKGCYASCLQASMAASNILGALV
HCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ATGVTLTLTREQIGDWGWRIPFILGLAIAPVGLWLRRTLDETPHFRAEMARAQHAHAEQK
HCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
APLLQVVRDHPRALAVGTGFSVLWAVCVYALVIYMPTHAQRALHFDGRDAFIASLVGNCL
CHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHHH
MAVTCVCAGSWSDRLGRRTVLAAGAALMLVSVYPLLRWLSDVHTLAALLTVQSAFCVLVA
HHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFTGVAPAALSELFPTRVRATGMSLSYNIATTIFGGFAPAILAWLTQQTGNPFAPAWYVM
HHHCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VASAIALASIAALSSTPRHA
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]