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The map label for this gene is hsrA [H]

Identifier: 237812276

GI number: 237812276

Start: 1972218

End: 1973621

Strand: Reverse

Name: hsrA [H]

Synonym: GBP346_A2024

Alternate gene names: 237812276

Gene position: 1973621-1972218 (Counterclockwise)

Preceding gene: 237812277

Following gene: 237812273

Centisome position: 48.15

GC content: 74.93

Gene sequence:

>1404_bases
ATGCGCGCCGGCGACTTTCGCGTGACCGCGGCCGTCGTCGCGAGCGCGCTCTTCATGCAGAACCTCGACAGCACGGTCGT
CGCGACCGCGCTGCCGAGCATGGCGCGCGAGCTCGGCGTAAACGTCGTGTTCCTGAGCAGCGCGATCACGTCGCACCTCG
TCGCGCTGACGGTGTTCATTCCGGTGAGCGGCTGGATCGCGGAGCGCTTCGGCGCGAAGCGCGTGTTCATCGCCGCGATC
GCGATCTTCACGGCGGCGTCCGTGATGTGCGCGGCGGCGAACGGGCTCGCGACGCTGGTCGCCGCGCGCATCCTGCAGGG
CGCGGGCGGCGCGCTGATGGTGCCCGTCGGCCGCCTGATCCTCTATCGCGGCGTGTCGCGCCACGAGATGCTCGCGGCGA
CCACCTGGCTCACGATGCCCGCGCTCGTCGGCCCGCTGCTCGGGCCGCCGCTCGGCGGCTTCTTGACCGATGCGCTGTCG
TGGCGCGCGGTGTTCTGGATCAACGTGCCGGTCGGCGTCGCGGGCGCGGCGCTCGCCGCGCGCCTCGTCCCCGCGTCGGC
CGGCGAGCGTCGCGCGCCCGCCGACGCGCGCGGCATGCTGCTCGTCGGCGCGGCACTCGCCGCGCTGATGCTCGGCGTCG
AGACCGCGGGCCGCGACGTGCTGCCGGCGGGGGCGCCCGCCCTGTGCCTCGGCGCGGGCGTCGCGCTCGGCGGGCTCACG
ATCCGCCATTGCCGGCGCGTCGCGCATCCGGTCGTCGATCTGTCGCTGCTCGGCATTCCGACCTTTCACGCGGCGACGAT
CGCGGGCAGCCTGTTCCGCGCGGGGGCCGGCGCGCTGCCGTTTCTCGTGCCGCTCACGCTGCAGGTCGGCTTCGGCGCGA
GCGCGTCGAGAAGCGGCGCCATCACGCTCGCGAGCGCGCTCGGCTCGCTCGTGATGCGGCCGATGACGCATGCGGCGCTG
CATCGCGCGCCGATGCGCACGGTGCTGATCGCGGGCAGCGTGTCGTTCGCGGCCGTGCTCGCCGCGTGCGCGACGCTATC
GCCCGCGTGGCCGGATGCGGCCGTCTTCGCGCTGCTGCTCGTCGGCGGGCTGTCGCGCTCGCTCAGCTTCGCGTCGCTCG
GCGCGCTCGTATTCTCGGACGTGCCGAGCGAGCGCCTGTCGGCCGCGACGTCGTTCCAGGGAACCGCGCAGCAGTTGATG
CGGGCGGTGGGCGTCGCCGTCGCGGCGGGCGCGCTGCATCTGGCGATGCTGATCGGCGGGCGCGGCCAGGCGAGCCGGAC
GGATTTCGCCTGCGCGTTCGCGGCGATCGCGCTCGTCGTGCTCGCGTCGGTGCCGATGTTCGCCGCGCTGCCGGCCGACG
CCGGCGAAGGGCTCGCGGGGCCGGCGCGCCGGCCGAGGCCATGA

Upstream 100 bases:

>100_bases
GCCGCGCGCGAGCGCGCGTCGTGATCGTGAACGCCGCGCCGCGCTGCCCGCCGCCGCCCCGCCGGGTTCGGCGGGGCGGC
GCCATGCGGGGCGAACCCTG

Downstream 100 bases:

>100_bases
TGCGGATCGCGTGACGATGCGGGGCGTGCGCGCGGCGTATCGAGCATGCCGACGCGGCCGGGCGGCGGCCGGATAGCCGG
GGCAAGTCGGCAAGTCGGCA

Product: drug resistance transporter, EmrB/QacA family

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MRAGDFRVTAAVVASALFMQNLDSTVVATALPSMARELGVNVVFLSSAITSHLVALTVFIPVSGWIAERFGAKRVFIAAI
AIFTAASVMCAAANGLATLVAARILQGAGGALMVPVGRLILYRGVSRHEMLAATTWLTMPALVGPLLGPPLGGFLTDALS
WRAVFWINVPVGVAGAALAARLVPASAGERRAPADARGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLT
IRHCRRVAHPVVDLSLLGIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGAITLASALGSLVMRPMTHAAL
HRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLLVGGLSRSLSFASLGALVFSDVPSERLSAATSFQGTAQQLM
RAVGVAVAAGALHLAMLIGGRGQASRTDFACAFAAIALVVLASVPMFAALPADAGEGLAGPARRPRP

Sequences:

>Translated_467_residues
MRAGDFRVTAAVVASALFMQNLDSTVVATALPSMARELGVNVVFLSSAITSHLVALTVFIPVSGWIAERFGAKRVFIAAI
AIFTAASVMCAAANGLATLVAARILQGAGGALMVPVGRLILYRGVSRHEMLAATTWLTMPALVGPLLGPPLGGFLTDALS
WRAVFWINVPVGVAGAALAARLVPASAGERRAPADARGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLT
IRHCRRVAHPVVDLSLLGIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGAITLASALGSLVMRPMTHAAL
HRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLLVGGLSRSLSFASLGALVFSDVPSERLSAATSFQGTAQQLM
RAVGVAVAAGALHLAMLIGGRGQASRTDFACAFAAIALVVLASVPMFAALPADAGEGLAGPARRPRP
>Mature_467_residues
MRAGDFRVTAAVVASALFMQNLDSTVVATALPSMARELGVNVVFLSSAITSHLVALTVFIPVSGWIAERFGAKRVFIAAI
AIFTAASVMCAAANGLATLVAARILQGAGGALMVPVGRLILYRGVSRHEMLAATTWLTMPALVGPLLGPPLGGFLTDALS
WRAVFWINVPVGVAGAALAARLVPASAGERRAPADARGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLT
IRHCRRVAHPVVDLSLLGIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGAITLASALGSLVMRPMTHAAL
HRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLLVGGLSRSLSFASLGALVFSDVPSERLSAATSFQGTAQQLM
RAVGVAVAAGALHLAMLIGGRGQASRTDFACAFAAIALVVLASVPMFAALPADAGEGLAGPARRPRP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=456, Percent_Identity=35.0877192982456, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788392, Length=465, Percent_Identity=34.8387096774194, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789042, Length=404, Percent_Identity=26.7326732673267, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788710, Length=417, Percent_Identity=25.6594724220624, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=25.7756563245823, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=128, Percent_Identity=32.03125, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47043; Mature: 47043

Theoretical pI: Translated: 11.97; Mature: 11.97

Prosite motif: PS00626 RCC1_2 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGDFRVTAAVVASALFMQNLDSTVVATALPSMARELGVNVVFLSSAITSHLVALTVFI
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
PVSGWIAERFGAKRVFIAAIAIFTAASVMCAAANGLATLVAARILQGAGGALMVPVGRLI
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LYRGVSRHEMLAATTWLTMPALVGPLLGPPLGGFLTDALSWRAVFWINVPVGVAGAALAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHH
RLVPASAGERRAPADARGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
IRHCRRVAHPVVDLSLLGIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCCH
ITLASALGSLVMRPMTHAALHRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLL
HHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHH
VGGLSRSLSFASLGALVFSDVPSERLSAATSFQGTAQQLMRAVGVAVAAGALHLAMLIGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGQASRTDFACAFAAIALVVLASVPMFAALPADAGEGLAGPARRPRP
CCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAGDFRVTAAVVASALFMQNLDSTVVATALPSMARELGVNVVFLSSAITSHLVALTVFI
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHH
PVSGWIAERFGAKRVFIAAIAIFTAASVMCAAANGLATLVAARILQGAGGALMVPVGRLI
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHH
LYRGVSRHEMLAATTWLTMPALVGPLLGPPLGGFLTDALSWRAVFWINVPVGVAGAALAA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCHHHHHHHHHH
RLVPASAGERRAPADARGMLLVGAALAALMLGVETAGRDVLPAGAPALCLGAGVALGGLT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
IRHCRRVAHPVVDLSLLGIPTFHAATIAGSLFRAGAGALPFLVPLTLQVGFGASASRSGA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCCCCCCH
ITLASALGSLVMRPMTHAALHRAPMRTVLIAGSVSFAAVLAACATLSPAWPDAAVFALLL
HHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHH
VGGLSRSLSFASLGALVFSDVPSERLSAATSFQGTAQQLMRAVGVAVAAGALHLAMLIGG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RGQASRTDFACAFAAIALVVLASVPMFAALPADAGEGLAGPARRPRP
CCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]