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The map label for this gene is oprA [H]

Identifier: 237812183

GI number: 237812183

Start: 1871125

End: 1872675

Strand: Direct

Name: oprA [H]

Synonym: GBP346_A1929

Alternate gene names: 237812183

Gene position: 1871125-1872675 (Clockwise)

Preceding gene: 237812182

Following gene: 237812184

Centisome position: 45.65

GC content: 74.15

Gene sequence:

>1551_bases
GTGAAAGCGATGATAAAACCCCGGGCCCGGCGGCGCGGCGCGCGCGCAGCGCGGCGGCCGAACGGGCCGCGGGCCTGGCC
GCTCGCGGTCGCGGCCGCGCTCGTCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCGGCCGGCCGCGCCCGTGCCGGCGC
ATTATTGGTCGACGGCGGCGGGCGCGCCGCGCGAGGCGGGCCCGGCGGCCGCCGGCGGCCCGCGCGCGATGCCGGACGCG
CGCCGCGGCGACGCCGGGCGCGACGCCCGCGACGCGCGGCTCGACGACTGGCGCGCGTATTTCACCGATCCGGCGCTGCG
CGCGCTGATCGACGCGGCGCTCGCGAACAATCGCGACCTGCGGATCGCGACGCTGCGTATCCAGGAAGCGCGCGGCCTCT
ACGGCGTCGCGCGCGCGGACCGGCTGCCGTCGATCGACGGCAGCCTCGGTTACGAGCGCACGCGGCTTTACGACCCGGTG
CTGCGCGAGAGCGCGACGAGCTCGCTGTATCGCGCGAGTGTCGGCGTGAGCGCGTTCGAGATCGATCTGTTCGGCCGCGT
GAAGAGCCTCTCGGACGCGGCGCTCGCCGAGTACTTCGCGACCGCCGAGGCGCAGCGCGCGGTGCGCATCAGCCTGATCG
CCGAAGTCGCGTCCGCGTACGTGACCGAGCGTGCGCTCGTCGATCAACTGGGGCTCGCCGAACGCACGCTCGCCGCGCGC
GACGCGGCCTACGCGCTCACGCAGCGCCGTTACGCGGCCGGCACGAGCACGGCGATCGAGCTGCGCACGGCCGAGATGCT
CGTCGCGTCGGCGCGCGCGTCGAAGGCGGCGCTCGAGCGCGAGCACACGCAGGCGGCGAGCGCGCTGAAGCTGCTCGCGG
GCGACTTCATGACGGCGCTGCCCGCCGATGCGCCCGCGCTCGACGCGCTCGCGGTGGCGCGGGTGTCGCCGGGGCTGTCG
TCGGATCTGCTCGAGCAGCGCCCGGACATCCGGCAGGCCGAGCAGCGCCTCGTCGCGGCGAACGCGAACATCGGCGCGGC
GCGCGCGGCGTTCTTCCCGCGCATCGCGCTCACGACCGACGTCGGCTCGGTGAGCGACGCGTTCTCGGGACTCTTCTCGG
CCGGCTCGAGCGTGTGGACGTTCGCGCCGCGGCTCACGCTGCCGATTTTCGCGGGCGGGCGCAATCGTGCGAATCTCGAC
GTGGCGGACGCACGCAAGCATATCGCGGTCGCCGAATACGAGAAGACGATCCAGACGGCGTTTCGCGAAGTCGCCGATGC
GCTCGCGGCGCGCGATCAGATCGACGCGCAGCTTGCCGCGCAGCAGGCGGTCTACGGTGCGGACGCCGAGCGGCTGCGGC
TTGCGCAGCGGCGCTACGACAGCGGCGTGGCGAGCTATCTCGAGCTGCTCGACGCGCAGCGCAGCACGTTCGAGTCCGGG
CAGGAGCTGATTCGCCTGAAGCAACTGCGGCTCACGAACGCGATCACGCTGTATCGCGCGCTGGGCGGGGGCTGGTCGCG
CGCCGGGTGCGGCGGCGATGAGTGTGCTTGA

Upstream 100 bases:

>100_bases
TCGCGATCTTCCTCGTCCCGCTCTTTTTCGTCTGCGTCGGGCGCGTGTTCGACGTCGTTCCGCGCCGCCGAGGCGGCGCG
CAAGCGGCACTGGAGGCCAA

Downstream 100 bases:

>100_bases
GCGCTTGAGCGCGCTGTGCATCGTGCATCGTGCATCGTGCATCGTGTGGCATCGATGCGCGACTTTTGTTCACCACTATG
GCGTGTTGCCGTTCCTTTTC

Product: outer membrane efflux protein OprA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKAMIKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAAGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAVRISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDECA

Sequences:

>Translated_516_residues
MKAMIKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAAGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAVRISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDECA
>Mature_516_residues
MKAMIKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAAGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAVRISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDECA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=482, Percent_Identity=41.2863070539419, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI2367348, Length=433, Percent_Identity=27.0207852193995, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55192; Mature: 55192

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMIKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAA
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
GAPREAGPAAAGGPRAMPDARRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
RIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGVSAFE
EEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDLFGRVKSLSDAALAEYFATAEAQRAVRISLIAEVASAYVTERALVDQLGLAERTLAAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTAL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADAPALDALAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTD
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
VGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTA
CCHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDECA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKAMIKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAA
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
GAPREAGPAAAGGPRAMPDARRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
RIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGVSAFE
EEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDLFGRVKSLSDAALAEYFATAEAQRAVRISLIAEVASAYVTERALVDQLGLAERTLAAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTAL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADAPALDALAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTD
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
VGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTA
CCHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDECA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]