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The map label for this gene is dusA [H]

Identifier: 237812120

GI number: 237812120

Start: 1809857

End: 1811176

Strand: Reverse

Name: dusA [H]

Synonym: GBP346_A1866

Alternate gene names: 237812120

Gene position: 1811176-1809857 (Counterclockwise)

Preceding gene: 237812122

Following gene: 237812113

Centisome position: 44.19

GC content: 66.89

Gene sequence:

>1320_bases
ATGCACGCCCCGCCTTCGAACGCTTCGAGCGCGCCGCATCGACGTGAAAACCGAAGGCGCGCGGCCCGTCGGCAGCGTGC
GAGGCACGGGCTGCGCGCTTCGCGGATTGTCCGAATGCGAACCTCCGCGCCTGCTCGGCCGAAGCGAGCAAACGCCGTTC
CGGCGCGCGGCGCCATTTCCTTGCGCATGATTTCGCCTGCGCGCACGAAGAGCCGCACCCGCCGCGCACGCCGGCTCGAC
GGCCGCGTTGCGGTAAAATGTTCGGTTGTCCCTGCGCCCCGACGCTTTTCCGCCCCGCCTATGTCCGCTTCGCCTGCCCT
CAGTCCACGCCGGGTCTCCGTCGCCCCGATGATGGACTGGACCGATCGCCACTGCCGTTCGTTTCATCGGACGATCTCGC
GCCATGCATGGCTCTACACCGAAATGGTGACGACGGGCGCGCTGATCCACGGCGATGTCGCGCGCCATCTCGCGTTCACG
CCCGACGAAGCTCCCGTTGCGTTGCAACTGGGCGGCAGCGAACCCGCCGATCTCGCGCACTCCGCGAAACTCGGCGAGCG
CTGGGGCTACGACGAAATCAACCTGAATTGCGGTTGCCCGTCCGAGCGCGTGCAGCGCGGCGCATTCGGCGCGTGCCTGA
TGAACGAGCCGCAACTCGTCGCGGACTGCGTGAAGGCGATGCGCGACGCGGTGTCGATCCCGGTGACGGTCAAGCACCGG
ATCGGCGTCGACGCGGTCGAAGACTACGCGTTCGTGCGCGACTTCGTCGGCACGGTCGCCGCGGCCGGCTGCGACGTGTT
CGTCGTGCACGCGCGCAACGCGATCCTGAAGGGGCTCTCGCCGAAGGAAAACCGCGAGATTCCGCCGCTCAAGTACGACT
ACGCGTATCGGCTCAAGCGCGACTTTCCGCAGATCGAAATCGTCATCAACGGCGGCGTCACGACGCTCGACGAAGTCGAG
CAGCATCTGCGACATGTCGACGGCGTGATGCTCGGCCGCGAGGCATATCACAACCCGTACGTGCTCGCCGGCATCGACGC
ACGCTTCTACGGCGCGAGCGCGCCGGCGCCGACGCGCGAAGAGGTGGAGGCGAAGCTCGTCGAATACTGCGCGGCCGAGC
TCGCGCGCGGCACGTATCTCGGGGCGATCGTGCGGCATGCGCTCGGCCTGTATCGAGGCGTCGCGGGCGCGCGCGGCTGG
CGTCGCGTGCTGTCAGACAACAAGCGGCTCGGGCGCGGCGACCTATCGATCTTCGACGAGGCGCGCACGCACCTGACCCA
TCCGGAAGAATTTTTTGAAAAAAAGGCTTTGCAAAGTTAA

Upstream 100 bases:

>100_bases
CCACGCCGGCGGCCCGCCTCCCGCCCATCGCCGGCGTTCGCCGGCCGCCTTTTTAGTCGGCGGCGGCCGCGTTGCCGCGC
CGGCATCGTGAACCGGAATC

Downstream 100 bases:

>100_bases
ACGGCCTTTGTATAATCTCGCTTCTTCGTTGATGCCGCTGCTGCGGCGAATCAAACGGAGAAAAAAATAGTGGTGGCTGT
AGCTCAGTTGGTAGAGTCCA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MHAPPSNASSAPHRRENRRRAARRQRARHGLRASRIVRMRTSAPARPKRANAVPARGAISLRMISPARTKSRTRRARRLD
GRVAVKCSVVPAPRRFSAPPMSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFT
PDEAPVALQLGGSEPADLAHSAKLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHR
IGVDAVEDYAFVRDFVGTVAAAGCDVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQIEIVINGGVTTLDEVE
QHLRHVDGVMLGREAYHNPYVLAGIDARFYGASAPAPTREEVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGW
RRVLSDNKRLGRGDLSIFDEARTHLTHPEEFFEKKALQS

Sequences:

>Translated_439_residues
MHAPPSNASSAPHRRENRRRAARRQRARHGLRASRIVRMRTSAPARPKRANAVPARGAISLRMISPARTKSRTRRARRLD
GRVAVKCSVVPAPRRFSAPPMSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFT
PDEAPVALQLGGSEPADLAHSAKLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHR
IGVDAVEDYAFVRDFVGTVAAAGCDVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQIEIVINGGVTTLDEVE
QHLRHVDGVMLGREAYHNPYVLAGIDARFYGASAPAPTREEVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGW
RRVLSDNKRLGRGDLSIFDEARTHLTHPEEFFEKKALQS
>Mature_439_residues
MHAPPSNASSAPHRRENRRRAARRQRARHGLRASRIVRMRTSAPARPKRANAVPARGAISLRMISPARTKSRTRRARRLD
GRVAVKCSVVPAPRRFSAPPMSASPALSPRRVSVAPMMDWTDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFT
PDEAPVALQLGGSEPADLAHSAKLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHR
IGVDAVEDYAFVRDFVGTVAAAGCDVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKRDFPQIEIVINGGVTTLDEVE
QHLRHVDGVMLGREAYHNPYVLAGIDARFYGASAPAPTREEVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGW
RRVLSDNKRLGRGDLSIFDEARTHLTHPEEFFEKKALQS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=29.535864978903, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=244, Percent_Identity=28.2786885245902, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=58.974358974359, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1788462, Length=287, Percent_Identity=27.8745644599303, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789660, Length=240, Percent_Identity=24.1666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=28.8372093023256, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=24.896265560166, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24580595, Length=293, Percent_Identity=25.5972696245734, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19920448, Length=293, Percent_Identity=25.5972696245734, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 48685; Mature: 48685

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAPPSNASSAPHRRENRRRAARRQRARHGLRASRIVRMRTSAPARPKRANAVPARGAIS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCCCCEE
LRMISPARTKSRTRRARRLDGRVAVKCSVVPAPRRFSAPPMSASPALSPRRVSVAPMMDW
EEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
TDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFTPDEAPVALQLGGSEPADLAH
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
SAKLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHR
HHHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEECC
IGVDAVEDYAFVRDFVGTVAAAGCDVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKR
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
DFPQIEIVINGGVTTLDEVEQHLRHVDGVMLGREAYHNPYVLAGIDARFYGASAPAPTRE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCEECCCCCCCCCHH
EVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDNKRLGRGDLSIFDE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ARTHLTHPEEFFEKKALQS
HHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHAPPSNASSAPHRRENRRRAARRQRARHGLRASRIVRMRTSAPARPKRANAVPARGAIS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCCCCEE
LRMISPARTKSRTRRARRLDGRVAVKCSVVPAPRRFSAPPMSASPALSPRRVSVAPMMDW
EEEECCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
TDRHCRSFHRTISRHAWLYTEMVTTGALIHGDVARHLAFTPDEAPVALQLGGSEPADLAH
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
SAKLGERWGYDEINLNCGCPSERVQRGAFGACLMNEPQLVADCVKAMRDAVSIPVTVKHR
HHHHHHHCCCCCEEEECCCCHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEECC
IGVDAVEDYAFVRDFVGTVAAAGCDVFVVHARNAILKGLSPKENREIPPLKYDYAYRLKR
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
DFPQIEIVINGGVTTLDEVEQHLRHVDGVMLGREAYHNPYVLAGIDARFYGASAPAPTRE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHCCCCEEEEECCCEECCCCCCCCCHH
EVEAKLVEYCAAELARGTYLGAIVRHALGLYRGVAGARGWRRVLSDNKRLGRGDLSIFDE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ARTHLTHPEEFFEKKALQS
HHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]