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The map label for this gene is 237811848

Identifier: 237811848

GI number: 237811848

Start: 1529796

End: 1531274

Strand: Reverse

Name: 237811848

Synonym: GBP346_A1587

Alternate gene names: NA

Gene position: 1531274-1529796 (Counterclockwise)

Preceding gene: 237811849

Following gene: 237811846

Centisome position: 37.36

GC content: 66.6

Gene sequence:

>1479_bases
ATGACCAGGAACGCAAGACGCACATGGCAATGGATCAGCGGACTGCTTGCGATATGCGCGGCCGCGCTCGCCGCGCTTCC
CGCGCGCGCCGACGTCGCCGCGGGGCCGGGCGCATGGAGCAGCCAGCAAACGTGGGGCGCGGATACCGTCAACGGCGGCA
ACCTGACGGGCTACTTCTATTGGCCCGCGACCCAGCCGACGACGCCCAACGGCAAGCGCGCGCTCGTGCTCGTCCTGCAC
GGCTGTCTGCAGAGCGCGTCGGGCGACGTCATCGACAATTCGCGAGGCGCGGGCTTCAACTGGAAAAGCGTCGCGGATCG
ATACGGCGCGATCATTCTGGCGCCCAACGCGACCGGAAACGTCTACGGCAACCATTGCTGGGACTATGCGAACACGTCGC
CGAATCGCGCGAGCGGCCACGTCGGCGTGTTGCTCGATCTCGTCAGCCGCTTCGTCGGCGACGCGCAATACGCGATCGAC
CCGAACCAGGTCTACGTGGCCGGCCTGTCTTCCGGCGGCGGCATGACGATGGTGCTCGGCTGCATCGCGCCGGACATCTT
CGCCGGCATCGGCATCAACGCCGGGCCGCCGCCCGGCACGACCACGCTGCAAATCGGCTACGTGCCGTCCGGCTACACCG
CTCAGACGGCGGCCAACCAATGCAAGGCATGGGCCGGCTCGAAGGCGGACCAGTTCTCCACCCAGATCGCGGGCGCCGTA
TGGGGCACGTCGGATTACACCGTCGCGCAGGCGTACGGCCCGCTCGACACCGCCGCGTTCCGCCTGATCTACGGCGGCAC
GTTTGCGCAGGGGTCCAAAGTATCGATCGCCGGCGGCGGCACCAACACGCCGTACTCGGACAGCAACGGCAAAGTGCGCA
CGCACGAGATCGTGGTTTCCGGCATGGCGCATGCGTGGCCCGCCGGTGCGGGCGGCGACAACGCGAATTACGTGGACGCG
ACGCACGTCAACTATCCGGCCTTCGTGATGGACTACTGGGTCAAGAACAATTTGCGCGCGAACGGCGCGCCGGTGCAGGC
CGGCACGCCGCCGACGGGGCTGACCGTCACGAACGCGACGCAGACGAGCATATCGCTCGCCTGGAACCCGGTGGCCAATG
CGAGCAGCTACAACGTCTACCGTAACGGCAACAAGGTCGGTTCATCGACGTCGACGGCCTACACCGATGCCGGGCTGATC
GCCGGCACGGCCTATTCCTATACGGTCACCGAGATCGATCCGAGCTTGGGCGAAAGCGCGCCGTCGCCCGCGGTGTCGGC
GACGACGCAACCGAGCTTCGCCTGCAGCGCGACCACCGCCACCAATTACGCGCACGTGCAGGCCGGCCGCGCGCACGATT
CGCTCGGCATCGCCTATGCGACCGGATCGAACCAGAACATGGGGCTCGACAACGTGTTTTATACGAACACGCTGGCGCAA
ACGAGCGCCGGTTACTACATCATCGGCGATTGTCCGTGA

Upstream 100 bases:

>100_bases
ATCGAAACGCGACGAACGACGCCGTCGCCTAAAAAGGTAGGACTGGAAAGCAAAAAAGGCCGTGGGTAACGACAACATTC
CCCATTCAAGGAGAGCCAGC

Downstream 100 bases:

>100_bases
GCCGGCGCCCGGCACATCGGCCGGCCGGCAGCCGGCGCGCTTCGCGCATGCGCGAAGCCGCGGCGATGGCCATCGAAGCC
GCCTTCGTCACGCGCCTCTC

Product: poly(3-hydroxybutyrate) depolymerase (PHBdepolymerase)

Products: NA

Alternate protein names: PHB depolymerase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTRNARRTWQWISGLLAICAAALAALPARADVAAGPGAWSSQQTWGADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLH
GCLQSASGDVIDNSRGAGFNWKSVADRYGAIILAPNATGNVYGNHCWDYANTSPNRASGHVGVLLDLVSRFVGDAQYAID
PNQVYVAGLSSGGGMTMVLGCIAPDIFAGIGINAGPPPGTTTLQIGYVPSGYTAQTAANQCKAWAGSKADQFSTQIAGAV
WGTSDYTVAQAYGPLDTAAFRLIYGGTFAQGSKVSIAGGGTNTPYSDSNGKVRTHEIVVSGMAHAWPAGAGGDNANYVDA
THVNYPAFVMDYWVKNNLRANGAPVQAGTPPTGLTVTNATQTSISLAWNPVANASSYNVYRNGNKVGSSTSTAYTDAGLI
AGTAYSYTVTEIDPSLGESAPSPAVSATTQPSFACSATTATNYAHVQAGRAHDSLGIAYATGSNQNMGLDNVFYTNTLAQ
TSAGYYIIGDCP

Sequences:

>Translated_492_residues
MTRNARRTWQWISGLLAICAAALAALPARADVAAGPGAWSSQQTWGADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLH
GCLQSASGDVIDNSRGAGFNWKSVADRYGAIILAPNATGNVYGNHCWDYANTSPNRASGHVGVLLDLVSRFVGDAQYAID
PNQVYVAGLSSGGGMTMVLGCIAPDIFAGIGINAGPPPGTTTLQIGYVPSGYTAQTAANQCKAWAGSKADQFSTQIAGAV
WGTSDYTVAQAYGPLDTAAFRLIYGGTFAQGSKVSIAGGGTNTPYSDSNGKVRTHEIVVSGMAHAWPAGAGGDNANYVDA
THVNYPAFVMDYWVKNNLRANGAPVQAGTPPTGLTVTNATQTSISLAWNPVANASSYNVYRNGNKVGSSTSTAYTDAGLI
AGTAYSYTVTEIDPSLGESAPSPAVSATTQPSFACSATTATNYAHVQAGRAHDSLGIAYATGSNQNMGLDNVFYTNTLAQ
TSAGYYIIGDCP
>Mature_491_residues
TRNARRTWQWISGLLAICAAALAALPARADVAAGPGAWSSQQTWGADTVNGGNLTGYFYWPATQPTTPNGKRALVLVLHG
CLQSASGDVIDNSRGAGFNWKSVADRYGAIILAPNATGNVYGNHCWDYANTSPNRASGHVGVLLDLVSRFVGDAQYAIDP
NQVYVAGLSSGGGMTMVLGCIAPDIFAGIGINAGPPPGTTTLQIGYVPSGYTAQTAANQCKAWAGSKADQFSTQIAGAVW
GTSDYTVAQAYGPLDTAAFRLIYGGTFAQGSKVSIAGGGTNTPYSDSNGKVRTHEIVVSGMAHAWPAGAGGDNANYVDAT
HVNYPAFVMDYWVKNNLRANGAPVQAGTPPTGLTVTNATQTSISLAWNPVANASSYNVYRNGNKVGSSTSTAYTDAGLIA
GTAYSYTVTEIDPSLGESAPSPAVSATTQPSFACSATTATNYAHVQAGRAHDSLGIAYATGSNQNMGLDNVFYTNTLAQT
SAGYYIIGDCP

Specific function: This protein degrades water-insoluble and water-soluble PHB to monomeric D(-)-3-hydroxybutyrate [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00041 fn3 [H]

EC number: =3.1.1.75 [H]

Molecular weight: Translated: 50775; Mature: 50644

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNARRTWQWISGLLAICAAALAALPARADVAAGPGAWSSQQTWGADTVNGGNLTGYFY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
WPATQPTTPNGKRALVLVLHGCLQSASGDVIDNSRGAGFNWKSVADRYGAIILAPNATGN
ECCCCCCCCCCCEEEEEEEHHHHHCCCCCEEECCCCCCCCHHHHHHHCCEEEECCCCCCC
VYGNHCWDYANTSPNRASGHVGVLLDLVSRFVGDAQYAIDPNQVYVAGLSSGGGMTMVLG
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCEEEHH
CIAPDIFAGIGINAGPPPGTTTLQIGYVPSGYTAQTAANQCKAWAGSKADQFSTQIAGAV
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEE
WGTSDYTVAQAYGPLDTAAFRLIYGGTFAQGSKVSIAGGGTNTPYSDSNGKVRTHEIVVS
ECCCCEEEEEECCCHHHHHEEEEECCEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEE
GMAHAWPAGAGGDNANYVDATHVNYPAFVMDYWVKNNLRANGAPVQAGTPPTGLTVTNAT
CHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
QTSISLAWNPVANASSYNVYRNGNKVGSSTSTAYTDAGLIAGTAYSYTVTEIDPSLGESA
CEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEECCCCEEECCEEEEEEEECCHHHCCCC
PSPAVSATTQPSFACSATTATNYAHVQAGRAHDSLGIAYATGSNQNMGLDNVFYTNTLAQ
CCCCEECCCCCCEEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECHHHH
TSAGYYIIGDCP
CCCCEEEEECCC
>Mature Secondary Structure 
TRNARRTWQWISGLLAICAAALAALPARADVAAGPGAWSSQQTWGADTVNGGNLTGYFY
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
WPATQPTTPNGKRALVLVLHGCLQSASGDVIDNSRGAGFNWKSVADRYGAIILAPNATGN
ECCCCCCCCCCCEEEEEEEHHHHHCCCCCEEECCCCCCCCHHHHHHHCCEEEECCCCCCC
VYGNHCWDYANTSPNRASGHVGVLLDLVSRFVGDAQYAIDPNQVYVAGLSSGGGMTMVLG
CCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCEEEHH
CIAPDIFAGIGINAGPPPGTTTLQIGYVPSGYTAQTAANQCKAWAGSKADQFSTQIAGAV
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEE
WGTSDYTVAQAYGPLDTAAFRLIYGGTFAQGSKVSIAGGGTNTPYSDSNGKVRTHEIVVS
ECCCCEEEEEECCCHHHHHEEEEECCEECCCCEEEEECCCCCCCCCCCCCCEEEEEEEEE
GMAHAWPAGAGGDNANYVDATHVNYPAFVMDYWVKNNLRANGAPVQAGTPPTGLTVTNAT
CHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCC
QTSISLAWNPVANASSYNVYRNGNKVGSSTSTAYTDAGLIAGTAYSYTVTEIDPSLGESA
CEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEECCCCEEECCEEEEEEEECCHHHCCCC
PSPAVSATTQPSFACSATTATNYAHVQAGRAHDSLGIAYATGSNQNMGLDNVFYTNTLAQ
CCCCEECCCCCCEEEECCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEECHHHH
TSAGYYIIGDCP
CCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644188; 1409594 [H]