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The map label for this gene is proP [H]

Identifier: 237811758

GI number: 237811758

Start: 1432934

End: 1434421

Strand: Reverse

Name: proP [H]

Synonym: GBP346_A1495

Alternate gene names: 237811758

Gene position: 1434421-1432934 (Counterclockwise)

Preceding gene: 237811761

Following gene: 237811743

Centisome position: 35.0

GC content: 70.23

Gene sequence:

>1488_bases
TTGACTGCAACACCCGCCCCCTCCAGTTCGTCCAGCGCGCCCACCGAAGGCGCGCTTCCCGCCGCTGCGCACGAGATCAC
CGTCGTCGATCAGGGCCTGCTCAAGCGCGCCGTCGGCGCGATGGCGCTCGGCAACGCGATGGAATGGTTCGACTTCGGCG
TCTACAGCTACATCGCCGTCACGCTCGGCCAGGTGTTCTTCCCGTCGAGCAGCCCGTCCGCGCAGTTGCTCGCGACGTTC
GGCACGTTCGCCGCCGCCTTCCTCGTGCGCCCGCTCGGCGGGATGGTGTTCGGGCCGCTCGGCGATCGCATCGGCCGCCA
GCGCGTGCTCGCGATGACGATGATCATGATGGCGGTCGGCACGTTCGCGATCGGCCTGATCCCGAGCTACGACTCGATCG
GCCTCCTCGCGCCCGTGCTGCTCCTCGTCGCGCGTCTCGTGCAAGGCTTCTCGACGGGCGGCGAGTACGGCGGCGCGGCA
ACCTTCATCGCCGAGTTCTCGACCGACAAGCGCCGCGGCTTCATGGGCAGCTTCCTCGAGTTCGGCACGCTGATCGGCTA
TGTGATGGGCGCGGGCGTCGTCGCGCTGCTGACGGCTTCGCTGTCGCACGACGCGCTGCTGTCGTGGGGCTGGCGCGTGC
CGTTCCTGATCGCCGGCCCGCTCGGCCTGATCGGCCTGTACATCCGGATGAGGCTCGAGGAAACGCCCGCGTTCAAGCGG
CAGGCCGAAGCGCGCGAAGCGCAGGACAAGGCCGTGCCGAAGGCGCATTTCCGCCGACAGCTCGCGCGGCACTGGCGCGC
GCTGCTGCTGTGCGTCGGCCTCGTGCTGATCTTCAACGTCACCGATTACATGGCGCTGTCGTACCTGCCGAGCTATCTGT
CGTCGACGCTGCACTTCGACGAGGCGCACGGCCTCGTGCTGATCCTGATCGTGATGGTGCTGATGATGCCGATGACGCTC
GCCACGGGCCGCCTGTCGGACGCCGTCGGCCGCAAGCCGGTGATGCTCGCCGGCTGCGTCGGGCTCTTCGCGCTCGCGAT
TCCCGCGCTGCTCCTGATCCGCACCGGCGAGACGGCGCTCGTGTTCGGCGGCCTGCTGATCCTCGGCGCACTGCTGTCGT
GCTTCACGGGCGTGATGCCGTCGGCGCTGCCCGCGCTCTTTCCGACCGAGATCCGCTACGGCGCGCTCGCGATCGGCTTC
AACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTCGCCGCCGCGTGGCTCGTCGACGCGACGGGCAACCTGATGAT
GCCCGCGTACTACCTGATGGGCGCGGCCGTGATCGGCGCGATCTCGGTGCTCGCGCTGCCCGAGAGCGCGCGCCAGCCGC
TCAAGGGCTCGCCGCCCGCCGTCGCGTCGCACCGCGAGGCACACGCGCTCGCGCGCGAGATCAAGCGCCGCGAGGCGGCC
GAGCGCGACAACAGCGGCTACCCGTCGGCCGCGGCGTTGCGCGCGTGA

Upstream 100 bases:

>100_bases
CGCGACCGACCCTCGTCCGATCGCGAGCGGCACTCCCGGCAAACCGTGGCGAACCGCGCCCCGTCGCGCGGTTCGGCCGC
CGTCACAGGAGTTTTCGACC

Downstream 100 bases:

>100_bases
GGTTCGGCCGCCGCGACGCCGCCGTGTAGGCGCGTCGCTCGATGACGGACCGCGGAGGCGGTCTCGCGATGCCCGTCGGG
CGTGGCGAGACCGCCTCCGC

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATF
GTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAA
TFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKR
QAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTL
ATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGF
NVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAISVLALPESARQPLKGSPPAVASHREAHALAREIKRREAA
ERDNSGYPSAAALRA

Sequences:

>Translated_495_residues
MTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATF
GTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAA
TFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKR
QAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTL
ATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGF
NVSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAISVLALPESARQPLKGSPPAVASHREAHALAREIKRREAA
ERDNSGYPSAAALRA
>Mature_494_residues
TATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAVTLGQVFFPSSSPSAQLLATFG
TFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVGTFAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAAT
FIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKRQ
AEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFDEAHGLVLILIVMVLMMPMTLA
TGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETALVFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFN
VSVSLFGGTTPLAAAWLVDATGNLMMPAYYLMGAAVIGAISVLALPESARQPLKGSPPAVASHREAHALAREIKRREAAE
RDNSGYPSAAALRA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=475, Percent_Identity=51.1578947368421, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1788292, Length=444, Percent_Identity=31.5315315315315, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788942, Length=382, Percent_Identity=34.8167539267016, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789941, Length=439, Percent_Identity=34.1685649202733, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082231, Length=210, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=24.6575342465753, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52256; Mature: 52125

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAV
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TLGQVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAMTMIMMAVG
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHH
TFAIGLIPSYDSIGLLAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMGSFLE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
FGTLIGYVMGAGVVALLTASLSHDALLSWGWRVPFLIAGPLGLIGLYIRMRLEETPAFKR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
QAEAREAQDKAVPKAHFRRQLARHWRALLLCVGLVLIFNVTDYMALSYLPSYLSSTLHFD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EAHGLVLILIVMVLMMPMTLATGRLSDAVGRKPVMLAGCVGLFALAIPALLLIRTGETAL
HHCCHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFGGLLILGALLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLAAAWLVD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEECEEEEEEECCCCHHHHHHHHH
ATGNLMMPAYYLMGAAVIGAISVLALPESARQPLKGSPPAVASHREAHALAREIKRREAA
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ERDNSGYPSAAALRA
HCCCCCCCCHHHCCC
>Mature Secondary Structure 
TATPAPSSSSSAPTEGALPAAAHEITVVDQGLLKRAVGAMALGNAMEWFDFGVYSYIAV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH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HCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]