Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 229918726

GI number: 229918726

Start: 2971701

End: 2972840

Strand: Direct

Name: cca [H]

Synonym: EAT1b_3013

Alternate gene names: 229918726

Gene position: 2971701-2972840 (Clockwise)

Preceding gene: 229918725

Following gene: 229918727

Centisome position: 99.06

GC content: 54.82

Gene sequence:

>1140_bases
ATGACAGAATGGGAATACGCAAAACAAATCATCGACACCATCGAACAGCATGGGGGCGAGGCGTATATCGTCGGTGGTGC
TGTTCGGGATTACGTGCTCGGGAGTCTGCCCGATGATATCGATTTGGCGACGTCCCTCTTCCCGGAGGAGGTGATGGCGC
TGTTCCCGAAGTCGGTTCCGACCGGTATTGAACATGGGACCGTCACGGTCATTCTCGACCACCATCCGTTCGAAGTGACG
ACATTCCGTTCAGAAGGGTCATATTCGGATTCGAGACGACCGGACCATGTCGCACTCGGGGTGTCGCTTGAAGAGGATCT
ATTACGAAGGGACTTCACGATGAACGCGATGGCGTTCCATGATGGGCGAGTGGTCGATTTATTCGGTGGCCAACATGATT
TAGAGGAACGAATCATCCGAACAGTCGGCAGTCCGTTTGAACGGTTCGGAGAGGACGCGCTTCGCATCATGCGCGCGCTT
CGCTTCATGTCACAGCTCGACTTCACCTTACATGACGAGTTGCGGGAAGCGGCGAGCGGGCTCGGTCATAAGCTCGAACA
GATTGCGATGGAGCGTATCGCCATCGAATTTGAGAAACTGTTGACGGGACCTGCGTACAAGAAAGCACTAGGTGAGATGG
TCGAGCTCGGCATTCATCGTCATTTACCAAACGTGACGACCGAACTGATGCAGCGTGTCCAAGAGGACACTCGGACCCCT
GTGAACGCGGTCAGCGGGTGGGCACTCTTTCTCTATCATGCACAGGATGACGGTTGGCTGTCGCCATGGAAACGGTCGAA
CGCCATCAAGCGAGAGGCACGCACACTTGCACAGCTCTGTGATACGGGGCTTGACCGGATGACGATTTATGCGACGGATG
AACAGACGCTCTCCACTTATTTCCGAATGAACGGGGAGAGAGACCGAAGCCGTGAACTGAAACAGTCGCTCCCGATGACG
TCAAGGAAAGAGTTAAAGTTTAACGGCCGGGACGCCTTAGCGCTCGGGGCACGAGGGGAAGCGATCAAGCATCTGTTAGC
CGACCTCGAACGACGCGTCATCTTAAACGAGTTACCGAACCATGAGACAGTATTGAAAGAGGAGGCGAAACGATGGCTTC
AACGCGCGGGCGTGTTCTGA

Upstream 100 bases:

>100_bases
GAGAACGGTTCCGACAAAATGGATTGGTGAAGTCCCGGCGTTTCGCCTCACAAGACATCGTGACCGAATATGAGCGCTTG
TATGAACAGGTGGTGACATC

Downstream 100 bases:

>100_bases
CGCTATTACAAGATGGTGAAGTATGGAGTGGTCAAGAGTTGGCACGCCAATTAAATATTTCACGTACCGCTATTTGGAAA
CATATCGAAACGCTTAAGCA

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTEWEYAKQIIDTIEQHGGEAYIVGGAVRDYVLGSLPDDIDLATSLFPEEVMALFPKSVPTGIEHGTVTVILDHHPFEVT
TFRSEGSYSDSRRPDHVALGVSLEEDLLRRDFTMNAMAFHDGRVVDLFGGQHDLEERIIRTVGSPFERFGEDALRIMRAL
RFMSQLDFTLHDELREAASGLGHKLEQIAMERIAIEFEKLLTGPAYKKALGEMVELGIHRHLPNVTTELMQRVQEDTRTP
VNAVSGWALFLYHAQDDGWLSPWKRSNAIKREARTLAQLCDTGLDRMTIYATDEQTLSTYFRMNGERDRSRELKQSLPMT
SRKELKFNGRDALALGARGEAIKHLLADLERRVILNELPNHETVLKEEAKRWLQRAGVF

Sequences:

>Translated_379_residues
MTEWEYAKQIIDTIEQHGGEAYIVGGAVRDYVLGSLPDDIDLATSLFPEEVMALFPKSVPTGIEHGTVTVILDHHPFEVT
TFRSEGSYSDSRRPDHVALGVSLEEDLLRRDFTMNAMAFHDGRVVDLFGGQHDLEERIIRTVGSPFERFGEDALRIMRAL
RFMSQLDFTLHDELREAASGLGHKLEQIAMERIAIEFEKLLTGPAYKKALGEMVELGIHRHLPNVTTELMQRVQEDTRTP
VNAVSGWALFLYHAQDDGWLSPWKRSNAIKREARTLAQLCDTGLDRMTIYATDEQTLSTYFRMNGERDRSRELKQSLPMT
SRKELKFNGRDALALGARGEAIKHLLADLERRVILNELPNHETVLKEEAKRWLQRAGVF
>Mature_378_residues
TEWEYAKQIIDTIEQHGGEAYIVGGAVRDYVLGSLPDDIDLATSLFPEEVMALFPKSVPTGIEHGTVTVILDHHPFEVTT
FRSEGSYSDSRRPDHVALGVSLEEDLLRRDFTMNAMAFHDGRVVDLFGGQHDLEERIIRTVGSPFERFGEDALRIMRALR
FMSQLDFTLHDELREAASGLGHKLEQIAMERIAIEFEKLLTGPAYKKALGEMVELGIHRHLPNVTTELMQRVQEDTRTPV
NAVSGWALFLYHAQDDGWLSPWKRSNAIKREARTLAQLCDTGLDRMTIYATDEQTLSTYFRMNGERDRSRELKQSLPMTS
RKELKFNGRDALALGARGEAIKHLLADLERRVILNELPNHETVLKEEAKRWLQRAGVF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=266, Percent_Identity=31.203007518797, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081691, Length=209, Percent_Identity=35.4066985645933, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789436, Length=193, Percent_Identity=35.2331606217617, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=214, Percent_Identity=35.5140186915888, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321016, Length=231, Percent_Identity=31.1688311688312, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281360112, Length=225, Percent_Identity=35.5555555555556, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21357337, Length=225, Percent_Identity=35.5555555555556, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43159; Mature: 43027

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEWEYAKQIIDTIEQHGGEAYIVGGAVRDYVLGSLPDDIDLATSLFPEEVMALFPKSVP
CCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCHHHHHHHCHHHHHHHCCCCCC
TGIEHGTVTVILDHHPFEVTTFRSEGSYSDSRRPDHVALGVSLEEDLLRRDFTMNAMAFH
CCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEC
DGRVVDLFGGQHDLEERIIRTVGSPFERFGEDALRIMRALRFMSQLDFTLHDELREAASG
CCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGHKLEQIAMERIAIEFEKLLTGPAYKKALGEMVELGIHRHLPNVTTELMQRVQEDTRTP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VNAVSGWALFLYHAQDDGWLSPWKRSNAIKREARTLAQLCDTGLDRMTIYATDEQTLSTY
HHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
FRMNGERDRSRELKQSLPMTSRKELKFNGRDALALGARGEAIKHLLADLERRVILNELPN
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
HETVLKEEAKRWLQRAGVF
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEWEYAKQIIDTIEQHGGEAYIVGGAVRDYVLGSLPDDIDLATSLFPEEVMALFPKSVP
CCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCHHHHHHHCHHHHHHHCCCCCC
TGIEHGTVTVILDHHPFEVTTFRSEGSYSDSRRPDHVALGVSLEEDLLRRDFTMNAMAFH
CCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCEEEC
DGRVVDLFGGQHDLEERIIRTVGSPFERFGEDALRIMRALRFMSQLDFTLHDELREAASG
CCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGHKLEQIAMERIAIEFEKLLTGPAYKKALGEMVELGIHRHLPNVTTELMQRVQEDTRTP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
VNAVSGWALFLYHAQDDGWLSPWKRSNAIKREARTLAQLCDTGLDRMTIYATDEQTLSTY
HHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
FRMNGERDRSRELKQSLPMTSRKELKFNGRDALALGARGEAIKHLLADLERRVILNELPN
HHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
HETVLKEEAKRWLQRAGVF
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA