Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 229918722

GI number: 229918722

Start: 2968414

End: 2968872

Strand: Direct

Name: greA [H]

Synonym: EAT1b_3009

Alternate gene names: 229918722

Gene position: 2968414-2968872 (Clockwise)

Preceding gene: 229918721

Following gene: 229918724

Centisome position: 98.95

GC content: 52.51

Gene sequence:

>459_bases
ATGAAACCGAAGCTGACCGAGACGGGTCATCACCAATTGACAGATCGACTTGTGATGCTAAGAAGAGAGCGGACGGATGC
GCGTGAACGGTTGCGGACAGCTCGAAAGTTTTGTGATTTCCGCGAAGACGTCACCTACACTGAAGCGGTACGTCAATATG
AGCGGATCGAGGCGGATATATTAGAACTGAATCACCTCCTTGACGACGCGACCATCGTCGAATCCGTTCAGTCTGAAGTC
GTGACGTTCGGAAATCGGGTCGTGATTCGGGAAGTGCAACAAGAAGAGACCGAGCGCTATCGAATCGTGAGCGAGGCAGA
AGCAGATCTAGAACGAGGAAGCATCTCCGTGACATCGCCACTCGGTCGTCGTTTGATGGGGGCGACAATGGGGCAAACGA
TTCAAGTGGAAACGCCAGGAGGTACGATTCAAGTCGAGGTGCTCCACATCGAACCATAA

Upstream 100 bases:

>100_bases
TTGGCACTCATCGTCTTTATGCCACGTGAGGAATAGGATTGAATGGAATCCCGTCGGTAGGCGGGATTTTTTTATTTTTT
GTTGAAAGGAAATGTTGTCA

Downstream 100 bases:

>100_bases
AAAATCGCCCGTTCATCCCTATGAACGAGGCGATTTTTTTGCTTATTTCAAAGGCATCCGATTTTCATCGAACGTAAACC
CTTCGCCCCCGACTTCATAC

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKPKLTETGHHQLTDRLVMLRRERTDARERLRTARKFCDFREDVTYTEAVRQYERIEADILELNHLLDDATIVESVQSEV
VTFGNRVVIREVQQEETERYRIVSEAEADLERGSISVTSPLGRRLMGATMGQTIQVETPGGTIQVEVLHIEP

Sequences:

>Translated_152_residues
MKPKLTETGHHQLTDRLVMLRRERTDARERLRTARKFCDFREDVTYTEAVRQYERIEADILELNHLLDDATIVESVQSEV
VTFGNRVVIREVQQEETERYRIVSEAEADLERGSISVTSPLGRRLMGATMGQTIQVETPGGTIQVEVLHIEP
>Mature_152_residues
MKPKLTETGHHQLTDRLVMLRRERTDARERLRTARKFCDFREDVTYTEAVRQYERIEADILELNHLLDDATIVESVQSEV
VTFGNRVVIREVQQEETERYRIVSEAEADLERGSISVTSPLGRRLMGATMGQTIQVETPGGTIQVEVLHIEP

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=151, Percent_Identity=29.1390728476821, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17507; Mature: 17507

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPKLTETGHHQLTDRLVMLRRERTDARERLRTARKFCDFREDVTYTEAVRQYERIEADI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LELNHLLDDATIVESVQSEVVTFGNRVVIREVQQEETERYRIVSEAEADLERGSISVTSP
HHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
LGRRLMGATMGQTIQVETPGGTIQVEVLHIEP
HHHHHHHHCCCCEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure
MKPKLTETGHHQLTDRLVMLRRERTDARERLRTARKFCDFREDVTYTEAVRQYERIEADI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LELNHLLDDATIVESVQSEVVTFGNRVVIREVQQEETERYRIVSEAEADLERGSISVTSP
HHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
LGRRLMGATMGQTIQVETPGGTIQVEVLHIEP
HHHHHHHHCCCCEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA