Click here to switch to the map view.

The map label for this gene is fliF [H]

Identifier: 229918627

GI number: 229918627

Start: 2876935

End: 2878506

Strand: Direct

Name: fliF [H]

Synonym: EAT1b_2914

Alternate gene names: 229918627

Gene position: 2876935-2878506 (Clockwise)

Preceding gene: 229918626

Following gene: 229918628

Centisome position: 95.9

GC content: 49.87

Gene sequence:

>1572_bases
ATGAATGAACAATTAAAAGAACGATTCAAATCCATGACCGATGCTCTCAAGGCGATGCCAGCGAAGCGTAAACTCGTATG
GGGCATCTCGATCGCGGTCATATTAATCGCGGCCCTCGTGCTCACATTCTTCCTTTCACGACCGAACATGGCGCCTCTCT
ATACAAACTTGTCTGCTCAAGAAGCAGGTGAAGTGACGGAGAAGTTGACAGCGGACGGCGTGCAGTCGGAAATCAAGGCG
ACTTCGAACGGTGTGAGCGTCTATGTGCCGACTGAAATGGTCGATCAGTTGAAAGTGAGTCTGGCTGCTGCCGGAATTCC
AAAATCCGGTTCAATTGACTACAGTTTCTTCAGTGAAAATGCAGGGTTCGGAACGACAGACCGTGAGATGGCTGTCATCG
AACGCAGTGCGATGCAGACGGAGCTCGAGCGACTCATCACACAAATCGAAGGAATCAATCAAGCGAAAGTCATCATCTCA
CTTCCTGAGAAATCTGTCTTCTTGGCAGAGGATCAAGGTGAGCCGACCGCATCCATCGTTTTGAACATCGGCGCGGGGAC
GAACTTAGAACCAAACACAGTAAAAGGCCTCTATCATTTGATTAGTAAGAGTGTCCCGAACTTGAAACCGGAAAACATCT
CAATCATGGACCAATACTTCACGTATTATGATCTCGACCAGACCGTTGTTGCTGGAGGTTCTTCATCGGATCCATTGGCG
CTCAAGCGACAAGTCGAAAAAGATGTCCGTCAACAAATTCAGCAGATGCTCTCGGTCCTACTCGGTGAACGGAGCGTACT
CGTTTCCGTCACAGCCGACATCGACACGACACAGACGGCTGAAGAACAGAATCTTGTCACACCCGTCGATGAAGAGAACA
TGGAAGGGATTGTGACATCTGCCGAGCGAATCGCTGAAGCGTACACTGGCGGAACAGGAACGGCGACAGAAGCGACGGGA
ACTGGTGAGAACGACACAATCAACTTCCCAGCGGCGACTGGCGAGGGTACAGAAGAAAGCGAAAAGAATCATGAAATTAT
TAATTACGAGGTCAATCGCATCACGAAACAGATTCAAAACGCACCGTACACGATTCGTGACCTCGGGGTACAACTCATCG
TTGAACCACCAGATGGCGCGACAAGCATCGATCCACAGCTAGAGGGCGACCTCGAGACGATGATGTATTCCATCATTCGG
ACGTCGTTAGCGAAGACGGACGAGGCCATCACGGATGCTGAACTTGAACAGAAAGTTGTCGTCGTGTCGAGACCGTTCGC
TGAAGTGGAAGAAGTCGCGGCAACAGAGCAACCGACACCTTGGTGGATGTATGCGGCAGCAGCAGGCGCAGTCGTACTCC
TTGCGTTGATCTTCTTCGCGATGCGTAGACGCAAGCAAGAAGTTGTGGAAGAGACGAACGAATGGGAAGTTCCATATGAA
GATGAAGTACCAGACCTAGACCTCGAAGAACGAGGCGAAGGTGCAGTGAAGAAGAAACAGTTAGAAAAATTAGCTGAAAG
TAATCCAGAAGAGTTTGCTAAACTGCTTCGTACTTGGATGTCTGAGGAGTGA

Upstream 100 bases:

>100_bases
ATTGCGATGCAACTTGCCATCGAGGTGAGGAACAAGGCTGTTGAGGCCTATCAAGAAATGATGCGAATGCAAGTTTAATT
GTAGAGGATGACACGTTGCG

Downstream 100 bases:

>100_bases
GTAACGTGAACTATGCAAAACAATCAAATCGCGAAAAAGCAGCGATGCTCATGATTTCCCTGGGACCTGAAGTGGCAGCG
AACGTCTATAAACATCTTTC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MNEQLKERFKSMTDALKAMPAKRKLVWGISIAVILIAALVLTFFLSRPNMAPLYTNLSAQEAGEVTEKLTADGVQSEIKA
TSNGVSVYVPTEMVDQLKVSLAAAGIPKSGSIDYSFFSENAGFGTTDREMAVIERSAMQTELERLITQIEGINQAKVIIS
LPEKSVFLAEDQGEPTASIVLNIGAGTNLEPNTVKGLYHLISKSVPNLKPENISIMDQYFTYYDLDQTVVAGGSSSDPLA
LKRQVEKDVRQQIQQMLSVLLGERSVLVSVTADIDTTQTAEEQNLVTPVDEENMEGIVTSAERIAEAYTGGTGTATEATG
TGENDTINFPAATGEGTEESEKNHEIINYEVNRITKQIQNAPYTIRDLGVQLIVEPPDGATSIDPQLEGDLETMMYSIIR
TSLAKTDEAITDAELEQKVVVVSRPFAEVEEVAATEQPTPWWMYAAAAGAVVLLALIFFAMRRRKQEVVEETNEWEVPYE
DEVPDLDLEERGEGAVKKKQLEKLAESNPEEFAKLLRTWMSEE

Sequences:

>Translated_523_residues
MNEQLKERFKSMTDALKAMPAKRKLVWGISIAVILIAALVLTFFLSRPNMAPLYTNLSAQEAGEVTEKLTADGVQSEIKA
TSNGVSVYVPTEMVDQLKVSLAAAGIPKSGSIDYSFFSENAGFGTTDREMAVIERSAMQTELERLITQIEGINQAKVIIS
LPEKSVFLAEDQGEPTASIVLNIGAGTNLEPNTVKGLYHLISKSVPNLKPENISIMDQYFTYYDLDQTVVAGGSSSDPLA
LKRQVEKDVRQQIQQMLSVLLGERSVLVSVTADIDTTQTAEEQNLVTPVDEENMEGIVTSAERIAEAYTGGTGTATEATG
TGENDTINFPAATGEGTEESEKNHEIINYEVNRITKQIQNAPYTIRDLGVQLIVEPPDGATSIDPQLEGDLETMMYSIIR
TSLAKTDEAITDAELEQKVVVVSRPFAEVEEVAATEQPTPWWMYAAAAGAVVLLALIFFAMRRRKQEVVEETNEWEVPYE
DEVPDLDLEERGEGAVKKKQLEKLAESNPEEFAKLLRTWMSEE
>Mature_523_residues
MNEQLKERFKSMTDALKAMPAKRKLVWGISIAVILIAALVLTFFLSRPNMAPLYTNLSAQEAGEVTEKLTADGVQSEIKA
TSNGVSVYVPTEMVDQLKVSLAAAGIPKSGSIDYSFFSENAGFGTTDREMAVIERSAMQTELERLITQIEGINQAKVIIS
LPEKSVFLAEDQGEPTASIVLNIGAGTNLEPNTVKGLYHLISKSVPNLKPENISIMDQYFTYYDLDQTVVAGGSSSDPLA
LKRQVEKDVRQQIQQMLSVLLGERSVLVSVTADIDTTQTAEEQNLVTPVDEENMEGIVTSAERIAEAYTGGTGTATEATG
TGENDTINFPAATGEGTEESEKNHEIINYEVNRITKQIQNAPYTIRDLGVQLIVEPPDGATSIDPQLEGDLETMMYSIIR
TSLAKTDEAITDAELEQKVVVVSRPFAEVEEVAATEQPTPWWMYAAAAGAVVLLALIFFAMRRRKQEVVEETNEWEVPYE
DEVPDLDLEERGEGAVKKKQLEKLAESNPEEFAKLLRTWMSEE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=386, Percent_Identity=26.4248704663212, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 57472; Mature: 57472

Theoretical pI: Translated: 4.14; Mature: 4.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQLKERFKSMTDALKAMPAKRKLVWGISIAVILIAALVLTFFLSRPNMAPLYTNLSAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
EAGEVTEKLTADGVQSEIKATSNGVSVYVPTEMVDQLKVSLAAAGIPKSGSIDYSFFSEN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
AGFGTTDREMAVIERSAMQTELERLITQIEGINQAKVIISLPEKSVFLAEDQGEPTASIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCEEEEE
LNIGAGTNLEPNTVKGLYHLISKSVPNLKPENISIMDQYFTYYDLDQTVVAGGSSSDPLA
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHH
LKRQVEKDVRQQIQQMLSVLLGERSVLVSVTADIDTTQTAEEQNLVTPVDEENMEGIVTS
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
AERIAEAYTGGTGTATEATGTGENDTINFPAATGEGTEESEKNHEIINYEVNRITKQIQN
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHC
APYTIRDLGVQLIVEPPDGATSIDPQLEGDLETMMYSIIRTSLAKTDEAITDAELEQKVV
CCCEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VVSRPFAEVEEVAATEQPTPWWMYAAAAGAVVLLALIFFAMRRRKQEVVEETNEWEVPYE
EEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DEVPDLDLEERGEGAVKKKQLEKLAESNPEEFAKLLRTWMSEE
CCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEQLKERFKSMTDALKAMPAKRKLVWGISIAVILIAALVLTFFLSRPNMAPLYTNLSAQ
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHH
EAGEVTEKLTADGVQSEIKATSNGVSVYVPTEMVDQLKVSLAAAGIPKSGSIDYSFFSEN
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
AGFGTTDREMAVIERSAMQTELERLITQIEGINQAKVIISLPEKSVFLAEDQGEPTASIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEECCCCCCEEEEE
LNIGAGTNLEPNTVKGLYHLISKSVPNLKPENISIMDQYFTYYDLDQTVVAGGSSSDPLA
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCHH
LKRQVEKDVRQQIQQMLSVLLGERSVLVSVTADIDTTQTAEEQNLVTPVDEENMEGIVTS
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
AERIAEAYTGGTGTATEATGTGENDTINFPAATGEGTEESEKNHEIINYEVNRITKQIQN
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHC
APYTIRDLGVQLIVEPPDGATSIDPQLEGDLETMMYSIIRTSLAKTDEAITDAELEQKVV
CCCEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VVSRPFAEVEEVAATEQPTPWWMYAAAAGAVVLLALIFFAMRRRKQEVVEETNEWEVPYE
EEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DEVPDLDLEERGEGAVKKKQLEKLAESNPEEFAKLLRTWMSEE
CCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1905667; 9384377; 1828465 [H]