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The map label for this gene is rnhB [H]

Identifier: 229918612

GI number: 229918612

Start: 2863338

End: 2864090

Strand: Direct

Name: rnhB [H]

Synonym: EAT1b_2899

Alternate gene names: 229918612

Gene position: 2863338-2864090 (Clockwise)

Preceding gene: 229918611

Following gene: 229918613

Centisome position: 95.45

GC content: 51.66

Gene sequence:

>753_bases
GTGACGATTCAACAGATTAAACAACAACTTCAACAAATCCGATATGAAGAGTGGAACCAATTGAAGTCGACGTTGTCACA
AGACCCACGTGCGGGCGTTCAGACATTGATTCGACAAAAAGAGCGTCAATTCGAACGGGAACAAGCGTTACGTGAGGATT
TCGAACGACGGATGATCTATGAGCGTGAGTGGAAGCATAAAGGGTATACGCGGGTCGTCGGCGTGGACGAGGTCGGACGG
GGGCCGCTTGCGGGACCGGTCGTGGCAGCAGCGGTCTTGTTGCCGGATGGGTTCTACCTCCCGGGATTGAATGATTCGAA
AAAAATGACCAAATCGGCGAGGGATGCGGCATACGCACACATCATCGAGGTCGCAGAGGTCGGTGTCGGGATTGTCGAGC
CCGCGATAATCGATGAGATTAACATCTATGAGGCGACGAAGCTTGCGATGACAGAGGCGATTCATCAAGTCGGAGAAGTG
GACGCCCTATTGATTGACGCCATGAAACTCGAGCTCGACATTCCCCAACAGTCTTTGATTAAAGGGGACACCCTCAGTGT
GTCAATAGCCGCTGCAAGTGTCGTCGCTAAGGTGGTGAGGGACCGGATGATGGAAGAATATGATTTGACGTATCCTGGAT
ACGGATTTGCGAAAAACGCAGGGTATGGGACAGAAACTCATTTAGAAGGGTTGAGACGCCTTGGGGTGACTCCGATCCAT
CGCCGTACATTCGCCCCCATCAAGTATATGTGA

Upstream 100 bases:

>100_bases
GACATGAAAAAATCGGACGGATCTCCCTTGAGACACCGGCTGATCACGAAATGGCGTAAAGCCCTGTGCACACAGGGCTT
TTTTGAATAGGAGGAAGACC

Downstream 100 bases:

>100_bases
AAGGAGTGGTCGCGTTGCGGATCGATCATCAAGGGGTGATGCCGTTCAAAGTGACGGAACAGACGTCACGCCCGCTACTA
CTAGGGAACACAGTCGTCGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTIQQIKQQLQQIRYEEWNQLKSTLSQDPRAGVQTLIRQKERQFEREQALREDFERRMIYEREWKHKGYTRVVGVDEVGR
GPLAGPVVAAAVLLPDGFYLPGLNDSKKMTKSARDAAYAHIIEVAEVGVGIVEPAIIDEINIYEATKLAMTEAIHQVGEV
DALLIDAMKLELDIPQQSLIKGDTLSVSIAAASVVAKVVRDRMMEEYDLTYPGYGFAKNAGYGTETHLEGLRRLGVTPIH
RRTFAPIKYM

Sequences:

>Translated_250_residues
MTIQQIKQQLQQIRYEEWNQLKSTLSQDPRAGVQTLIRQKERQFEREQALREDFERRMIYEREWKHKGYTRVVGVDEVGR
GPLAGPVVAAAVLLPDGFYLPGLNDSKKMTKSARDAAYAHIIEVAEVGVGIVEPAIIDEINIYEATKLAMTEAIHQVGEV
DALLIDAMKLELDIPQQSLIKGDTLSVSIAAASVVAKVVRDRMMEEYDLTYPGYGFAKNAGYGTETHLEGLRRLGVTPIH
RRTFAPIKYM
>Mature_249_residues
TIQQIKQQLQQIRYEEWNQLKSTLSQDPRAGVQTLIRQKERQFEREQALREDFERRMIYEREWKHKGYTRVVGVDEVGRG
PLAGPVVAAAVLLPDGFYLPGLNDSKKMTKSARDAAYAHIIEVAEVGVGIVEPAIIDEINIYEATKLAMTEAIHQVGEVD
ALLIDAMKLELDIPQQSLIKGDTLSVSIAAASVVAKVVRDRMMEEYDLTYPGYGFAKNAGYGTETHLEGLRRLGVTPIHR
RTFAPIKYM

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=47.2527472527472, Blast_Score=140, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 28202; Mature: 28071

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCC
>Mature Secondary Structure 
TIQQIKQQLQQIRYEEWNQLKSTLSQDPRAGVQTLIRQKERQFEREQALREDFERRMIY
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EREWKHKGYTRVVGVDEVGRGPLAGPVVAAAVLLPDGFYLPGLNDSKKMTKSARDAAYAH
HHHHCCCCCEEEEEHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
IIEVAEVGVGIVEPAIIDEINIYEATKLAMTEAIHQVGEVDALLIDAMKLELDIPQQSLI
HHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
KGDTLSVSIAAASVVAKVVRDRMMEEYDLTYPGYGFAKNAGYGTETHLEGLRRLGVTPIH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHH
RRTFAPIKYM
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA