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The map label for this gene is thiN [H]

Identifier: 229918588

GI number: 229918588

Start: 2841753

End: 2842340

Strand: Direct

Name: thiN [H]

Synonym: EAT1b_2875

Alternate gene names: 229918588

Gene position: 2841753-2842340 (Clockwise)

Preceding gene: 229918587

Following gene: 229918590

Centisome position: 94.73

GC content: 49.66

Gene sequence:

>588_bases
ATGAACATCTTGATCGTCGCGGCGAGTCCGGAATCTATTCCGGACTTACCGCGTGATGCCGATTTTGTCATCGGTGTCGA
TGGCGGATATCAGAGATTATTAGATGAAGGGATTGAAGTCGACGTCGTGGTCGGGGACTTTGATTCGTTTGTCGGAACAC
CTCCTGAGGAAGCGCTACGTTATCCATCTGAAAAGGATGTGACCGACCTCGAGATTGCGCTTCAGTTGGCAATCGATCAA
GGGGCGACCCAGATGGACGTGTACGGGGCACTCGGTGGTCGAGTTGATATGACGTTCGCGAACGTCGGTCTACTCGAATC
GTATCCGGGGATGGTTCTTCATGGGACGGGTCAAACGATTTTTCTCGCTCGGCCAGGAAGTTATGAGATTAAAAAACTAG
ATGAACATTACTTGTCTTTCATGCCTTGGAAAACCGCCACGATTTCAATTGATGGGGTCAAATATCCACTCGAGCGTCAT
CCGGTCCATAGCGCGCAAGCGTTGACCATTTCAAATGAATGGAGTGAAGAGTGTGCCTTGTTGACGGTCCATGAGGGAAT
GGTCCTCGTCATGCTCGTCAAAAAATAA

Upstream 100 bases:

>100_bases
CGTATATGGTTCCGTCGTTTGTCATCTCAGCCATCGTATTAGTCTTACTCTTCACGACAGCTCCGCGATTCACGCGTGTC
GCGACACGGGACATTCGTCA

Downstream 100 bases:

>100_bases
GCAAAAAAAATCCCATCGGCGAATGCCGCTGGGATTTATTCGTTCGTGTATAAGTATTATACGCGTTCAACGAGACCTGA
TTTAAGTGCGCGAGCTGAAA

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MNILIVAASPESIPDLPRDADFVIGVDGGYQRLLDEGIEVDVVVGDFDSFVGTPPEEALRYPSEKDVTDLEIALQLAIDQ
GATQMDVYGALGGRVDMTFANVGLLESYPGMVLHGTGQTIFLARPGSYEIKKLDEHYLSFMPWKTATISIDGVKYPLERH
PVHSAQALTISNEWSEECALLTVHEGMVLVMLVKK

Sequences:

>Translated_195_residues
MNILIVAASPESIPDLPRDADFVIGVDGGYQRLLDEGIEVDVVVGDFDSFVGTPPEEALRYPSEKDVTDLEIALQLAIDQ
GATQMDVYGALGGRVDMTFANVGLLESYPGMVLHGTGQTIFLARPGSYEIKKLDEHYLSFMPWKTATISIDGVKYPLERH
PVHSAQALTISNEWSEECALLTVHEGMVLVMLVKK
>Mature_195_residues
MNILIVAASPESIPDLPRDADFVIGVDGGYQRLLDEGIEVDVVVGDFDSFVGTPPEEALRYPSEKDVTDLEIALQLAIDQ
GATQMDVYGALGGRVDMTFANVGLLESYPGMVLHGTGQTIFLARPGSYEIKKLDEHYLSFMPWKTATISIDGVKYPLERH
PVHSAQALTISNEWSEECALLTVHEGMVLVMLVKK

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 21352; Mature: 21352

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIVAASPESIPDLPRDADFVIGVDGGYQRLLDEGIEVDVVVGDFDSFVGTPPEEALR
CEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCCEEEEEEECCHHHCCCCHHHHHC
YPSEKDVTDLEIALQLAIDQGATQMDVYGALGGRVDMTFANVGLLESYPGMVLHGTGQTI
CCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHCCCCEEEECCCCEE
FLARPGSYEIKKLDEHYLSFMPWKTATISIDGVKYPLERHPVHSAQALTISNEWSEECAL
EEECCCCCHHHHHHHHHHHHCCCCEEEEEECCEECCHHHCCCCCCEEEEECCCCCCCEEE
LTVHEGMVLVMLVKK
EEECCCEEEEEEEEC
>Mature Secondary Structure
MNILIVAASPESIPDLPRDADFVIGVDGGYQRLLDEGIEVDVVVGDFDSFVGTPPEEALR
CEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHCCCEEEEEEECCHHHCCCCHHHHHC
YPSEKDVTDLEIALQLAIDQGATQMDVYGALGGRVDMTFANVGLLESYPGMVLHGTGQTI
CCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHCCCCEEEECCCCEE
FLARPGSYEIKKLDEHYLSFMPWKTATISIDGVKYPLERHPVHSAQALTISNEWSEECAL
EEECCCCCHHHHHHHHHHHHCCCCEEEEEECCEECCHHHCCCCCCEEEEECCCCCCCEEE
LTVHEGMVLVMLVKK
EEECCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]