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The map label for this gene is murD [H]

Identifier: 229918549

GI number: 229918549

Start: 2799209

End: 2800546

Strand: Direct

Name: murD [H]

Synonym: EAT1b_2836

Alternate gene names: 229918549

Gene position: 2799209-2800546 (Clockwise)

Preceding gene: 229918548

Following gene: 229918550

Centisome position: 93.31

GC content: 51.2

Gene sequence:

>1338_bases
ATGGAAAAGCAATGGCACGGTAAAAAAGTTCTTGTCCTCGGTGCTGCGAAGAGCGGTATTGCGGCGGCGACATATCTGCA
TCATGCGGGAGCAGATGTGACGATCAATACGGGGAATATCCCTCCGGAAGACGAACAGGAAAAAATTCAGGCGCTCGGGA
TTCAATCGGTGTTTAACGAGCATCCCCTCGCTCTGTTGAATGAGACAGAGGTGATTGTCAAAAACCCAGGAATCCCGTAT
CAGATTCCATTGCTTCAGGAAGCGATGTCTCGTCATATCCCGATCTATACTGAAGTGGAACTTGCTTATTTAGCAACAGA
GGCGGAATTTGTTGCCATCACTGGCTCAAATGGGAAAACGACAACCACGACACTCGTCTATGAATTGCTCAAAGGTGGGG
AGCGTCCGGTGCATCTTGCGGGAAACATCGGATTCCCTGCGATTGAAGTGGCGTCTCAGGCGAAGGCGGGCGATTTGATT
GTGATTGAATTGTCGAGTTTTCAATTGATGGGGATTGAGTCGTTTCATCCTCGCTCGGCGGCACTACTCAATCTATCAGA
AGCTCATTTGGACTATCATGGGGATTTTGATGCGTATGCGGATGCAAAAGGAAACTTGTTCCGTCGAATGACGAATGCAG
ATCAAATCGTCGTGAAAGTGACAGATCCGGAAGTGATGAAACGTGTCCCGGGAGAGATTGAACCGACTACGTTCAGCTTT
GACCGGGGTGACGCTTCTGTCGTCGATGGATGGGTCACGGTTCGTGGTGAAAAAATCATGCCGGTAAATGAGTTGGCATT
AGGCGGGGCGCACAACGTGGAAAATGTATTGGCTGCACTACTCTTAGTGGAACCATTCGCTATTCCACGTACGCATATCG
AACGTGTATTACGAAACTTCGGTGGCGTCGCGCATCGGACCGAGAGTCTCGGTGATGTGCTCGGACGTGCCGTCTACAAT
GATTCGAAAGCGACGAACAACGTGGCAGCGGAAGCGGCGTTACGTGGGTTTGACGCCCCAATCGTCTGGCTCTGTGGTGG
TCTCGAGCGTGGTGCGGACTTATCTTCGCTCGCCCCAGCTCTCTCAAACGTAAAAGCTGTCGTCGCGTATGGTGAGACGA
AAGAGCGTTTTAAAGAGCTCGGCGAGTCCCGACAGATTCCATCTTTCACCGTCGAGACGTTGGCAGATGCCGTTCCGCTC
GCGTTTGAGCAATCCGAGACGGGAGACGTCATCTTACTCTCACCCGCTTGTGCTAGCTGGGATCAATTTAAAACGTTTGA
AGAACGAGGCGAGCAGTTCGTTCAACTCGTCCGTTCATATGAGGAGGCGAACCGATGA

Upstream 100 bases:

>100_bases
ATGGTCGGGTGGTCAGAGTGGCGCATCGTGCTCACGTTCGGTGGTATCAGTTTAATTACGGCAGGTTTATCATTACTACT
CATGTAAAAGGGTGAGACAG

Downstream 100 bases:

>100_bases
AAATCATGATTTCTGGTGGTGGTACCGGTGGTCATATTTATCCAGCACTCGCATTGATTGAAACGCTAAAAAAACGTCAC
CCGGATTTACAGGTACAATA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEKQWHGKKVLVLGAAKSGIAAATYLHHAGADVTINTGNIPPEDEQEKIQALGIQSVFNEHPLALLNETEVIVKNPGIPY
QIPLLQEAMSRHIPIYTEVELAYLATEAEFVAITGSNGKTTTTTLVYELLKGGERPVHLAGNIGFPAIEVASQAKAGDLI
VIELSSFQLMGIESFHPRSAALLNLSEAHLDYHGDFDAYADAKGNLFRRMTNADQIVVKVTDPEVMKRVPGEIEPTTFSF
DRGDASVVDGWVTVRGEKIMPVNELALGGAHNVENVLAALLLVEPFAIPRTHIERVLRNFGGVAHRTESLGDVLGRAVYN
DSKATNNVAAEAALRGFDAPIVWLCGGLERGADLSSLAPALSNVKAVVAYGETKERFKELGESRQIPSFTVETLADAVPL
AFEQSETGDVILLSPACASWDQFKTFEERGEQFVQLVRSYEEANR

Sequences:

>Translated_445_residues
MEKQWHGKKVLVLGAAKSGIAAATYLHHAGADVTINTGNIPPEDEQEKIQALGIQSVFNEHPLALLNETEVIVKNPGIPY
QIPLLQEAMSRHIPIYTEVELAYLATEAEFVAITGSNGKTTTTTLVYELLKGGERPVHLAGNIGFPAIEVASQAKAGDLI
VIELSSFQLMGIESFHPRSAALLNLSEAHLDYHGDFDAYADAKGNLFRRMTNADQIVVKVTDPEVMKRVPGEIEPTTFSF
DRGDASVVDGWVTVRGEKIMPVNELALGGAHNVENVLAALLLVEPFAIPRTHIERVLRNFGGVAHRTESLGDVLGRAVYN
DSKATNNVAAEAALRGFDAPIVWLCGGLERGADLSSLAPALSNVKAVVAYGETKERFKELGESRQIPSFTVETLADAVPL
AFEQSETGDVILLSPACASWDQFKTFEERGEQFVQLVRSYEEANR
>Mature_445_residues
MEKQWHGKKVLVLGAAKSGIAAATYLHHAGADVTINTGNIPPEDEQEKIQALGIQSVFNEHPLALLNETEVIVKNPGIPY
QIPLLQEAMSRHIPIYTEVELAYLATEAEFVAITGSNGKTTTTTLVYELLKGGERPVHLAGNIGFPAIEVASQAKAGDLI
VIELSSFQLMGIESFHPRSAALLNLSEAHLDYHGDFDAYADAKGNLFRRMTNADQIVVKVTDPEVMKRVPGEIEPTTFSF
DRGDASVVDGWVTVRGEKIMPVNELALGGAHNVENVLAALLLVEPFAIPRTHIERVLRNFGGVAHRTESLGDVLGRAVYN
DSKATNNVAAEAALRGFDAPIVWLCGGLERGADLSSLAPALSNVKAVVAYGETKERFKELGESRQIPSFTVETLADAVPL
AFEQSETGDVILLSPACASWDQFKTFEERGEQFVQLVRSYEEANR

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=448, Percent_Identity=34.5982142857143, Blast_Score=194, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48309; Mature: 48309

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQWHGKKVLVLGAAKSGIAAATYLHHAGADVTINTGNIPPEDEQEKIQALGIQSVFNE
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
HPLALLNETEVIVKNPGIPYQIPLLQEAMSRHIPIYTEVELAYLATEAEFVAITGSNGKT
CCEEEECCCEEEEECCCCCEECHHHHHHHHCCCCEEEEEEEEEEEECCEEEEEECCCCCC
TTTTLVYELLKGGERPVHLAGNIGFPAIEVASQAKAGDLIVIELSSFQLMGIESFHPRSA
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCC
ALLNLSEAHLDYHGDFDAYADAKGNLFRRMTNADQIVVKVTDPEVMKRVPGEIEPTTFSF
EEEEEHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCEEEE
DRGDASVVDGWVTVRGEKIMPVNELALGGAHNVENVLAALLLVEPFAIPRTHIERVLRNF
CCCCCCEEEEEEEECCCEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGVAHRTESLGDVLGRAVYNDSKATNNVAAEAALRGFDAPIVWLCGGLERGADLSSLAPA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
LSNVKAVVAYGETKERFKELGESRQIPSFTVETLADAVPLAFEQSETGDVILLSPACASW
HHCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEECCCCCCCEEEECCCCCCH
DQFKTFEERGEQFVQLVRSYEEANR
HHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKQWHGKKVLVLGAAKSGIAAATYLHHAGADVTINTGNIPPEDEQEKIQALGIQSVFNE
CCCCCCCCEEEEEECCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
HPLALLNETEVIVKNPGIPYQIPLLQEAMSRHIPIYTEVELAYLATEAEFVAITGSNGKT
CCEEEECCCEEEEECCCCCEECHHHHHHHHCCCCEEEEEEEEEEEECCEEEEEECCCCCC
TTTTLVYELLKGGERPVHLAGNIGFPAIEVASQAKAGDLIVIELSSFQLMGIESFHPRSA
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEECCEEEECCCCCCCCCC
ALLNLSEAHLDYHGDFDAYADAKGNLFRRMTNADQIVVKVTDPEVMKRVPGEIEPTTFSF
EEEEEHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCHHHHHHCCCCCCCCEEEE
DRGDASVVDGWVTVRGEKIMPVNELALGGAHNVENVLAALLLVEPFAIPRTHIERVLRNF
CCCCCCEEEEEEEECCCEECCHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GGVAHRTESLGDVLGRAVYNDSKATNNVAAEAALRGFDAPIVWLCGGLERGADLSSLAPA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHH
LSNVKAVVAYGETKERFKELGESRQIPSFTVETLADAVPLAFEQSETGDVILLSPACASW
HHCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEECCCCCCCEEEECCCCCCH
DQFKTFEERGEQFVQLVRSYEEANR
HHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA