Click here to switch to the map view.

The map label for this gene is nadE

Identifier: 229918486

GI number: 229918486

Start: 2738761

End: 2739576

Strand: Direct

Name: nadE

Synonym: EAT1b_2773

Alternate gene names: 229918486

Gene position: 2738761-2739576 (Clockwise)

Preceding gene: 229918485

Following gene: 229918488

Centisome position: 91.3

GC content: 50.37

Gene sequence:

>816_bases
ATGCAACAACATATCATTGAATCAACACATGTCAAACCGACCATCAACCCGAAAGAAGAAATTCGTCAACGTATCGACTT
CTTGAAAGCATATGTGAAACGGGCTGGTGCGAAAGGACTCGTCTTAGGGATCTCAGGCGGGCAAGACTCGAGCCTTGCCG
GAAAGCTATGTCAACTCGCCATGGAAGAGCTTCGAGAAGAAACGGGTGACGAGTATACATTTTATGCAATCAGATTGCCG
TATGGTGAGCAACACGATGAGCATGACGCGCAACTTGCGCTTGAGTTCATTCGTCCGGACAAATCGTTCACTGTCAACAT
CAAGCCTGCTGTTGACGCGTCGGTAGAAGCGTTTCACCGTGCAACGGGCCTCGAATTGAGTGACTTCCATAAAGGGAACA
CGAAGGCACGTGAGCGGATGAAGGCACAATACGACATTGCGGCAACGTTCGGTGCCCTTGTGGTCGGAACGGACCATGCC
GCAGAATATGTGACAGGATTTTATACAAAACATGGTGACGGAGCTTGTGACCTCACGCCGTTGACCGGCTTGAACAAACG
TCAAGGGAAAGCACTTCTCCATGAACTTGAAGCGCATGAGCGTGTGATTTATAAGGTGCCGACGGCCGACCTTGAAGATG
GGCGCCCTGGACTACCTGATGAAGTCGCGCTCGGTATGACATATGATCAGCTGGACGACTATTTGGAAGGGAAGACAATC
GATCCGACAATCGCCGAGCGAATCGAGACGATTTTCAAACGTTCACGCCACAAGCATCATATGCCTGCCTCGTTGTACGA
CGAATGGTGGCAATGA

Upstream 100 bases:

>100_bases
GAAACTGTTGTTTCCGTCTTTCTTTTTGAGACGCTAGTTGACGGGGTATAAGGATTATCAGACAATAAAGCAAAATGAGA
GCATAGTGGGAGGGTAATCC

Downstream 100 bases:

>100_bases
AAAAAGGAGGAGCTGTCTCGAGACAGCTTCTCCTTTGTTGTCTCACGCTTTTTTAAGTGGGATGAACTGAATGATCAGGA
TGCAGGTGAAAGCGATGAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQQHIIESTHVKPTINPKEEIRQRIDFLKAYVKRAGAKGLVLGISGGQDSSLAGKLCQLAMEELREETGDEYTFYAIRLP
YGEQHDEHDAQLALEFIRPDKSFTVNIKPAVDASVEAFHRATGLELSDFHKGNTKARERMKAQYDIAATFGALVVGTDHA
AEYVTGFYTKHGDGACDLTPLTGLNKRQGKALLHELEAHERVIYKVPTADLEDGRPGLPDEVALGMTYDQLDDYLEGKTI
DPTIAERIETIFKRSRHKHHMPASLYDEWWQ

Sequences:

>Translated_271_residues
MQQHIIESTHVKPTINPKEEIRQRIDFLKAYVKRAGAKGLVLGISGGQDSSLAGKLCQLAMEELREETGDEYTFYAIRLP
YGEQHDEHDAQLALEFIRPDKSFTVNIKPAVDASVEAFHRATGLELSDFHKGNTKARERMKAQYDIAATFGALVVGTDHA
AEYVTGFYTKHGDGACDLTPLTGLNKRQGKALLHELEAHERVIYKVPTADLEDGRPGLPDEVALGMTYDQLDDYLEGKTI
DPTIAERIETIFKRSRHKHHMPASLYDEWWQ
>Mature_271_residues
MQQHIIESTHVKPTINPKEEIRQRIDFLKAYVKRAGAKGLVLGISGGQDSSLAGKLCQLAMEELREETGDEYTFYAIRLP
YGEQHDEHDAQLALEFIRPDKSFTVNIKPAVDASVEAFHRATGLELSDFHKGNTKARERMKAQYDIAATFGALVVGTDHA
AEYVTGFYTKHGDGACDLTPLTGLNKRQGKALLHELEAHERVIYKVPTADLEDGRPGLPDEVALGMTYDQLDDYLEGKTI
DPTIAERIETIFKRSRHKHHMPASLYDEWWQ

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=273, Percent_Identity=58.2417582417582, Blast_Score=317, Evalue=7e-88,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_EXISA (C4L5A2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887132.1
- GeneID:   7870660
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2773
- OMA:   GLNKRRV
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30506; Mature: 30506

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQHIIESTHVKPTINPKEEIRQRIDFLKAYVKRAGAKGLVLGISGGQDSSLAGKLCQLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
MEELREETGDEYTFYAIRLPYGEQHDEHDAQLALEFIRPDKSFTVNIKPAVDASVEAFHR
HHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
ATGLELSDFHKGNTKARERMKAQYDIAATFGALVVGTDHAAEYVTGFYTKHGDGACDLTP
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHEECCCCCCCCCC
LTGLNKRQGKALLHELEAHERVIYKVPTADLEDGRPGLPDEVALGMTYDQLDDYLEGKTI
CCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCC
DPTIAERIETIFKRSRHKHHMPASLYDEWWQ
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure
MQQHIIESTHVKPTINPKEEIRQRIDFLKAYVKRAGAKGLVLGISGGQDSSLAGKLCQLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHH
MEELREETGDEYTFYAIRLPYGEQHDEHDAQLALEFIRPDKSFTVNIKPAVDASVEAFHR
HHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
ATGLELSDFHKGNTKARERMKAQYDIAATFGALVVGTDHAAEYVTGFYTKHGDGACDLTP
HCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHEECCCCCCCCCC
LTGLNKRQGKALLHELEAHERVIYKVPTADLEDGRPGLPDEVALGMTYDQLDDYLEGKTI
CCCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCC
DPTIAERIETIFKRSRHKHHMPASLYDEWWQ
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA