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The map label for this gene is dppE [H]

Identifier: 229918450

GI number: 229918450

Start: 2708612

End: 2710231

Strand: Direct

Name: dppE [H]

Synonym: EAT1b_2737

Alternate gene names: 229918450

Gene position: 2708612-2710231 (Clockwise)

Preceding gene: 229918447

Following gene: 229918451

Centisome position: 90.29

GC content: 45.12

Gene sequence:

>1620_bases
ATGAAGAAAAAATCGATTTTGCTCATGATGACGATCATCTTGGCACTCGGTTCAGTTCTCGCGGCTTGTTCAACAGGCGG
AGACTCTGATGGCGGCGGTTCTTCTTCAAACGGAGAAAAAGTTCTTCGTTTGACGGATACGTCAGATATCACAACAGCCG
ATCCGGCTCTTGCTACAGACGCGGTCGCATTCAACTTGATCGCCAACACAATGGAAGGTCTTTACCGTCTTGATAAAGAT
GGAAATGCAGTACCGGCACTTGCTGAAGGCGAACCAGAAGTAAACGAAGACGAGACGGTTTACACGTTCACACTTCGTGA
TGCTGAATGGTCAAACGGGGAACCGGTCACAGCTAATGACTTCGTTTATGCTTGGCAGCGAGCTGTCGATCCTGCAACAG
GTTCACAGTACGCATATATCATGAACACAATTAAAAACGCTGAAGCAATCAACACTGGTGACACACCAAAAGAAGAGCTC
GGTGTTAAAGCAATCGATGAGAAGACACTCGAAGTTACACTCGAGCGTCCAGACCCATCATTCCTTTCACTTACTTCGTT
CGGTACATTCACTCCGATCAACGAAGCATTCGCCACTGAAAAAGGGAAAGATTTCTCAACTAGCCCTGAAAACCTTCTCT
ACAACGGACCGTTCACATGGACTGAATGGGACCGCGAGCAAGGTTACGTCTTAACTAAAAACGACACGTATTGGGATAAA
GAGAACGTGGCCCTCGAAGCGGTTGACGTTCGTGTTGTTAAAGAAACATCTACAGTCGTAAACTTGTATGAAGCGGGCGA
TGTTGACTACGCTGGTCTCGCATCAGAACAAGTCGCAGCATTCCAAGAAGATGAGGACTACAACACGGGTCTTCGTTCTG
CAATCGGTTATTTCAAATTTAACCATGAAGATGAGATTTTCTCTGATGTAAACGCACGTAAAGCAATCGCACGCGCAGTT
GATCCATCAGGAATCATCGATCAGCTCTTGAACAACGGTTCAGTAGCGACAACGTCATTCATCCCGAAAGACTTCATCAA
GTATGAAGACGGAACGGATTACACAGAAGGCGTGGAATACTTCAAAACAAACGCTGACGAAGCTGCTTCACTCTGGGAAG
AGGCAACTGGTGGAGAAGCGACTACAATCGAACTCCTCTCGTTCGATAGCGAAGTATCGAAACAAATCTCTGAGTACATG
AAAGGTCAAATAGAGTCGAACTTACCGAACGTAACGGTTGAGATCGCACAACAACCTTTCAACAACAAACTTGAGCGTGA
AGCAAAAGGGGACTACCAAATGTCATTCGCACTTTGGGGACCTGACTACCAAGATCCGCTTACAAACCTTGGTATCTTCA
CTTCAGAGAATGGACAAAACGACATCAACTACTCATCATCTGAGTATGACAAGTTGATTGATGAGGCATCTGCTGAAACG
AGCATCGATGCACGTTATGACTTATTCAAAGAAGCAGAAGCTCTCTTGATTGAACAAGATCAAGCAATCATGCCAATCTA
CCAAGCAGGTGTGGCATACTTGATCCGTCCAAATATCGAAAACTTTAACCGTCAATTATTCGGTGCTGATTACCAGTACA
AATACGTTGATATTAAGTGA

Upstream 100 bases:

>100_bases
CTTTCGTCGAATACTTTATGAAATATTCAGAAAATTAAAGAAAAAGTAAGATGTTTATTCGGCGATCGGCTAGCTTTTCA
AAATGAAAGGGGAAATCGCA

Downstream 100 bases:

>100_bases
TGGAAGGAGGGGGCATGCTTAACGCATGTCCCCAATTTTCTAAAAAATTCTAATGAAGTAAGAGAAAACACTGTTCAAAT
GTTTTGTTGGTAAAGTATAA

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKKKSILLMMTIILALGSVLAACSTGGDSDGGGSSSNGEKVLRLTDTSDITTADPALATDAVAFNLIANTMEGLYRLDKD
GNAVPALAEGEPEVNEDETVYTFTLRDAEWSNGEPVTANDFVYAWQRAVDPATGSQYAYIMNTIKNAEAINTGDTPKEEL
GVKAIDEKTLEVTLERPDPSFLSLTSFGTFTPINEAFATEKGKDFSTSPENLLYNGPFTWTEWDREQGYVLTKNDTYWDK
ENVALEAVDVRVVKETSTVVNLYEAGDVDYAGLASEQVAAFQEDEDYNTGLRSAIGYFKFNHEDEIFSDVNARKAIARAV
DPSGIIDQLLNNGSVATTSFIPKDFIKYEDGTDYTEGVEYFKTNADEAASLWEEATGGEATTIELLSFDSEVSKQISEYM
KGQIESNLPNVTVEIAQQPFNNKLEREAKGDYQMSFALWGPDYQDPLTNLGIFTSENGQNDINYSSSEYDKLIDEASAET
SIDARYDLFKEAEALLIEQDQAIMPIYQAGVAYLIRPNIENFNRQLFGADYQYKYVDIK

Sequences:

>Translated_539_residues
MKKKSILLMMTIILALGSVLAACSTGGDSDGGGSSSNGEKVLRLTDTSDITTADPALATDAVAFNLIANTMEGLYRLDKD
GNAVPALAEGEPEVNEDETVYTFTLRDAEWSNGEPVTANDFVYAWQRAVDPATGSQYAYIMNTIKNAEAINTGDTPKEEL
GVKAIDEKTLEVTLERPDPSFLSLTSFGTFTPINEAFATEKGKDFSTSPENLLYNGPFTWTEWDREQGYVLTKNDTYWDK
ENVALEAVDVRVVKETSTVVNLYEAGDVDYAGLASEQVAAFQEDEDYNTGLRSAIGYFKFNHEDEIFSDVNARKAIARAV
DPSGIIDQLLNNGSVATTSFIPKDFIKYEDGTDYTEGVEYFKTNADEAASLWEEATGGEATTIELLSFDSEVSKQISEYM
KGQIESNLPNVTVEIAQQPFNNKLEREAKGDYQMSFALWGPDYQDPLTNLGIFTSENGQNDINYSSSEYDKLIDEASAET
SIDARYDLFKEAEALLIEQDQAIMPIYQAGVAYLIRPNIENFNRQLFGADYQYKYVDIK
>Mature_539_residues
MKKKSILLMMTIILALGSVLAACSTGGDSDGGGSSSNGEKVLRLTDTSDITTADPALATDAVAFNLIANTMEGLYRLDKD
GNAVPALAEGEPEVNEDETVYTFTLRDAEWSNGEPVTANDFVYAWQRAVDPATGSQYAYIMNTIKNAEAINTGDTPKEEL
GVKAIDEKTLEVTLERPDPSFLSLTSFGTFTPINEAFATEKGKDFSTSPENLLYNGPFTWTEWDREQGYVLTKNDTYWDK
ENVALEAVDVRVVKETSTVVNLYEAGDVDYAGLASEQVAAFQEDEDYNTGLRSAIGYFKFNHEDEIFSDVNARKAIARAV
DPSGIIDQLLNNGSVATTSFIPKDFIKYEDGTDYTEGVEYFKTNADEAASLWEEATGGEATTIELLSFDSEVSKQISEYM
KGQIESNLPNVTVEIAQQPFNNKLEREAKGDYQMSFALWGPDYQDPLTNLGIFTSENGQNDINYSSSEYDKLIDEASAET
SIDARYDLFKEAEALLIEQDQAIMPIYQAGVAYLIRPNIENFNRQLFGADYQYKYVDIK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=30.4924242424242, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=28.8201160541586, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87081878, Length=477, Percent_Identity=30.6079664570231, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787762, Length=501, Percent_Identity=23.9520958083832, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789966, Length=479, Percent_Identity=23.5908141962422, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789887, Length=493, Percent_Identity=23.1237322515213, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787052, Length=299, Percent_Identity=22.0735785953177, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59632; Mature: 59632

Theoretical pI: Translated: 3.91; Mature: 3.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKSILLMMTIILALGSVLAACSTGGDSDGGGSSSNGEKVLRLTDTSDITTADPALATD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHH
AVAFNLIANTMEGLYRLDKDGNAVPALAEGEPEVNEDETVYTFTLRDAEWSNGEPVTAND
HHHHHHHHHHHHHHHEECCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCH
FVYAWQRAVDPATGSQYAYIMNTIKNAEAINTGDTPKEELGVKAIDEKTLEVTLERPDPS
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEECCCEEEEEEECCCCC
FLSLTSFGTFTPINEAFATEKGKDFSTSPENLLYNGPFTWTEWDREQGYVLTKNDTYWDK
EEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCC
ENVALEAVDVRVVKETSTVVNLYEAGDVDYAGLASEQVAAFQEDEDYNTGLRSAIGYFKF
CCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHEEE
NHEDEIFSDVNARKAIARAVDPSGIIDQLLNNGSVATTSFIPKDFIKYEDGTDYTEGVEY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCHHHHHHH
FKTNADEAASLWEEATGGEATTIELLSFDSEVSKQISEYMKGQIESNLPNVTVEIAQQPF
HHCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NNKLEREAKGDYQMSFALWGPDYQDPLTNLGIFTSENGQNDINYSSSEYDKLIDEASAET
CHHHHHHCCCCEEEEEEEECCCCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCC
SIDARYDLFKEAEALLIEQDQAIMPIYQAGVAYLIRPNIENFNRQLFGADYQYKYVDIK
CCHHHHHHHHHHHHHEEECCCCCHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MKKKSILLMMTIILALGSVLAACSTGGDSDGGGSSSNGEKVLRLTDTSDITTADPALATD
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHH
AVAFNLIANTMEGLYRLDKDGNAVPALAEGEPEVNEDETVYTFTLRDAEWSNGEPVTAND
HHHHHHHHHHHHHHHEECCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCH
FVYAWQRAVDPATGSQYAYIMNTIKNAEAINTGDTPKEELGVKAIDEKTLEVTLERPDPS
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEECCCEEEEEEECCCCC
FLSLTSFGTFTPINEAFATEKGKDFSTSPENLLYNGPFTWTEWDREQGYVLTKNDTYWDK
EEEEECCCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCEEEECCCCCCCC
ENVALEAVDVRVVKETSTVVNLYEAGDVDYAGLASEQVAAFQEDEDYNTGLRSAIGYFKF
CCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHEEE
NHEDEIFSDVNARKAIARAVDPSGIIDQLLNNGSVATTSFIPKDFIKYEDGTDYTEGVEY
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCHHHHHHH
FKTNADEAASLWEEATGGEATTIELLSFDSEVSKQISEYMKGQIESNLPNVTVEIAQQPF
HHCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
NNKLEREAKGDYQMSFALWGPDYQDPLTNLGIFTSENGQNDINYSSSEYDKLIDEASAET
CHHHHHHCCCCEEEEEEEECCCCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHCCCC
SIDARYDLFKEAEALLIEQDQAIMPIYQAGVAYLIRPNIENFNRQLFGADYQYKYVDIK
CCHHHHHHHHHHHHHEEECCCCCHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]