Click here to switch to the map view.

The map label for this gene is apt

Identifier: 229918415

GI number: 229918415

Start: 2670843

End: 2671361

Strand: Direct

Name: apt

Synonym: EAT1b_2701

Alternate gene names: 229918415

Gene position: 2670843-2671361 (Clockwise)

Preceding gene: 229918414

Following gene: 229918416

Centisome position: 89.03

GC content: 48.17

Gene sequence:

>519_bases
ATGGATTTCAAACAGCATATTAAAGAAGTAGCAGATTACCCGAAGGAAGGAATCAGCTTCAAAGACATCACGTCACTCAT
GCAAAATGGCGAAGTCTACAAGAAATCGGTAGATGAGCTCGTCGCTTATGCACGTGAACGTGGAGCCGAGTTGATCGCTG
GACCGGAAGCGCGCGGATTTGTCGTCGGTTGCCCGGCAGCATATGCACTTGAACTCGGGTTCGTACCTGTACGTAAAGAA
GGAAAGTTACCACGTGAGACAGTACGTGTATCATACGGTCTCGAGTACGGAACAGACATCTTAACGATGCACAAAGATTC
AATCCAACCTGGACAACAAGTTGTCATCTTGGATGACTTGCTCGCAACAGGTGGTACGATTGAAGCGACGATTAAAATGA
TTGAACAACTCGGTGGAGTTGTGGCGGGTATCGGGTTCTTGATCGAACTCGATGGACTTGGTGGACGTGAGCGTCTCGAA
GGATACGACGTGTTCTCACTCATCCGCTACGAAGACTAA

Upstream 100 bases:

>100_bases
CAACAACTCGAAGAGCGTTTCGTATACGCGTCATTGGCGGAGCTCAAAGAGCGGTTACAATCGTTGCGATCGAACAAGAT
GCAGGAGGCATACACATAAA

Downstream 100 bases:

>100_bases
TTTGAACGGGAAGCGACATGCTTCCCGTTTTTGAATAGTTTTTGAGGAAAATAACCTACAACCTTTGACAGGTCGTCAGA
TTTTTTCGATAATGGTTATA

Product: adenine phosphoribosyltransferase

Products: NA

Alternate protein names: APRT

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MDFKQHIKEVADYPKEGISFKDITSLMQNGEVYKKSVDELVAYARERGAELIAGPEARGFVVGCPAAYALELGFVPVRKE
GKLPRETVRVSYGLEYGTDILTMHKDSIQPGQQVVILDDLLATGGTIEATIKMIEQLGGVVAGIGFLIELDGLGGRERLE
GYDVFSLIRYED

Sequences:

>Translated_172_residues
MDFKQHIKEVADYPKEGISFKDITSLMQNGEVYKKSVDELVAYARERGAELIAGPEARGFVVGCPAAYALELGFVPVRKE
GKLPRETVRVSYGLEYGTDILTMHKDSIQPGQQVVILDDLLATGGTIEATIKMIEQLGGVVAGIGFLIELDGLGGRERLE
GYDVFSLIRYED
>Mature_172_residues
MDFKQHIKEVADYPKEGISFKDITSLMQNGEVYKKSVDELVAYARERGAELIAGPEARGFVVGCPAAYALELGFVPVRKE
GKLPRETVRVSYGLEYGTDILTMHKDSIQPGQQVVILDDLLATGGTIEATIKMIEQLGGVVAGIGFLIELDGLGGRERLE
GYDVFSLIRYED

Specific function: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis

COG id: COG0503

COG function: function code F; Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family

Homologues:

Organism=Homo sapiens, GI4502171, Length=171, Percent_Identity=39.1812865497076, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI71773201, Length=124, Percent_Identity=39.5161290322581, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1786675, Length=170, Percent_Identity=52.9411764705882, Blast_Score=177, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17509087, Length=170, Percent_Identity=40.5882352941176, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323619, Length=172, Percent_Identity=37.7906976744186, Blast_Score=111, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320649, Length=172, Percent_Identity=33.7209302325581, Blast_Score=86, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17136334, Length=169, Percent_Identity=45.5621301775148, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): APT_EXISA (C4L531)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887061.1
- ProteinModelPortal:   C4L531
- GeneID:   7868905
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2701
- OMA:   GILFYDI
- ProtClustDB:   PRK02304
- GO:   GO:0005737
- HAMAP:   MF_00004
- InterPro:   IPR005764
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01090

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.7

Molecular weight: Translated: 18886; Mature: 18886

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKQHIKEVADYPKEGISFKDITSLMQNGEVYKKSVDELVAYARERGAELIAGPEARGF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
VVGCPAAYALELGFVPVRKEGKLPRETVRVSYGLEYGTDILTMHKDSIQPGQQVVILDDL
EEECCHHHHHEECCEEECCCCCCCHHHEEEEECCCCCCEEEEEECCCCCCCCEEEEEEHH
LATGGTIEATIKMIEQLGGVVAGIGFLIELDGLGGRERLEGYDVFSLIRYED
HHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHCCCCHHHEEEECC
>Mature Secondary Structure
MDFKQHIKEVADYPKEGISFKDITSLMQNGEVYKKSVDELVAYARERGAELIAGPEARGF
CCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCE
VVGCPAAYALELGFVPVRKEGKLPRETVRVSYGLEYGTDILTMHKDSIQPGQQVVILDDL
EEECCHHHHHEECCEEECCCCCCCHHHEEEEECCCCCCEEEEEECCCCCCCCEEEEEEHH
LATGGTIEATIKMIEQLGGVVAGIGFLIELDGLGGRERLEGYDVFSLIRYED
HHCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHCCCCHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA