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The map label for this gene is cycB [H]

Identifier: 229918222

GI number: 229918222

Start: 2465983

End: 2467248

Strand: Direct

Name: cycB [H]

Synonym: EAT1b_2502

Alternate gene names: 229918222

Gene position: 2465983-2467248 (Clockwise)

Preceding gene: 229918219

Following gene: 229918223

Centisome position: 82.2

GC content: 48.03

Gene sequence:

>1266_bases
ATGAAGATGAAAAAAATGGTAGCAGGACTTTCAACAGCAGTATTCGCATTCGGTGCACTTGCTGCATGTGGTGGCGGAAC
GGACAACGGATCTTCTAACGAAGGTGGTTCTTCAAGCGAGAACAAACCTGAAAAGATCGTCATCTGGGAAGACATCGAAA
AGTCTGAAACAACGAAAGAAGTTGCGAAGGCGTTCGAAGAAGAGCATGGCGTAAAAGTTGAAGTTGTCGAAGTACAGATG
ACAGACCAAAAAGACAAGCTTGCGCTTGACGGTCCAGCTGGAAAAGGTCCAGACATCGTGCTCGTACCACACGACCAGAT
CGGTACAATCGCAGACCAAGGTCACTTGGCTCCAATCGCGGACGAAGGATCACTTGACGCGTTCACAGACGCTGCGAAAT
CAGCTGTCATGTTCGATGGTCAAGCTTACGGATATCCAAAATCAGTTGAGACACCAGTTCTCATGTACAACAAAGACTTG
ATGGCTGAAGCTCCAGCATCTATGGACGACCTTTACAAGTTGTCAAACGATGTGAAAGCTGACGGACAGTACGGATTCCT
CGCACTTTGGGATAACTTCTACTTCGCACACGGTGTTGTCGCTGGATTCGGCGGATACGTGTTCAAAGAAGACGGTGGCG
CACTCGACCCAGCGGATATCGGTTTGAATAACGAAGGCGCAGTTGAAGGATTCGAATACATCGGTAAATGGTACGAAGAA
GGCCTCTTCCCTAAAGGGTTGATCGGTGAGTCTGGCGGCCAAGCGATGGACCAACTCTTCACTGAGAAGAAAGCTCATTC
AGTCATGAACGGACCATGGGCTGTAGCTGGTTACACAGATGCAGGCGTGAACCTCGGAGCGGCTCCAATGCCAACACTTC
CAAACGGTGAGCCAATCAAGACGTTCATGGGTGTGAAAACATATGCCCTTTCAGCTTACACAGAAAACCAAGAGTGGGCT
GAAATGTTCCTTCAAGAGTTGACGAATGAAGAAAACGCATTGGCAATGTTTGAAGCATACAACGAAATCCCACCAGTAGC
AGCACTCGAGTCGAACGAGACAATCACGTCGAACGAAGTTGCCAAAGCGGTATTCGACCAAGCAACAAACGCAATCCCAA
TGCCGAACATTCCTGAAATGGGACAAGTTTGGGAGCCAATGGCACAAGCACTTCAGCTTGTAGCAACTGGTAAACAAGAT
GCACAAAAATCTGCAGACGATGCAGTGAAAGTCATCGAACAACAAATCCAAGCGAACAACCAATAA

Upstream 100 bases:

>100_bases
TTTCACTACCGTGTCAATTTCGTAGCGGAGTTTTAAAATTGACGTGGTCGTGCGATTGCGCAAATCTGACCATACTATTT
CCTTGGGGAGGTTTTTCATC

Downstream 100 bases:

>100_bases
GGAATTGAGATGACGGGGTACGCCACATGGCGTTCCCCATCATTTTCTAGCATTGAGCAAACGTTTGCTTGATTGATTTG
CTCAAAACGAAAGGAGAAAC

Product: extracellular solute-binding protein family 1

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKMKKMVAGLSTAVFAFGALAACGGGTDNGSSNEGGSSSENKPEKIVIWEDIEKSETTKEVAKAFEEEHGVKVEVVEVQM
TDQKDKLALDGPAGKGPDIVLVPHDQIGTIADQGHLAPIADEGSLDAFTDAAKSAVMFDGQAYGYPKSVETPVLMYNKDL
MAEAPASMDDLYKLSNDVKADGQYGFLALWDNFYFAHGVVAGFGGYVFKEDGGALDPADIGLNNEGAVEGFEYIGKWYEE
GLFPKGLIGESGGQAMDQLFTEKKAHSVMNGPWAVAGYTDAGVNLGAAPMPTLPNGEPIKTFMGVKTYALSAYTENQEWA
EMFLQELTNEENALAMFEAYNEIPPVAALESNETITSNEVAKAVFDQATNAIPMPNIPEMGQVWEPMAQALQLVATGKQD
AQKSADDAVKVIEQQIQANNQ

Sequences:

>Translated_421_residues
MKMKKMVAGLSTAVFAFGALAACGGGTDNGSSNEGGSSSENKPEKIVIWEDIEKSETTKEVAKAFEEEHGVKVEVVEVQM
TDQKDKLALDGPAGKGPDIVLVPHDQIGTIADQGHLAPIADEGSLDAFTDAAKSAVMFDGQAYGYPKSVETPVLMYNKDL
MAEAPASMDDLYKLSNDVKADGQYGFLALWDNFYFAHGVVAGFGGYVFKEDGGALDPADIGLNNEGAVEGFEYIGKWYEE
GLFPKGLIGESGGQAMDQLFTEKKAHSVMNGPWAVAGYTDAGVNLGAAPMPTLPNGEPIKTFMGVKTYALSAYTENQEWA
EMFLQELTNEENALAMFEAYNEIPPVAALESNETITSNEVAKAVFDQATNAIPMPNIPEMGQVWEPMAQALQLVATGKQD
AQKSADDAVKVIEQQIQANNQ
>Mature_421_residues
MKMKKMVAGLSTAVFAFGALAACGGGTDNGSSNEGGSSSENKPEKIVIWEDIEKSETTKEVAKAFEEEHGVKVEVVEVQM
TDQKDKLALDGPAGKGPDIVLVPHDQIGTIADQGHLAPIADEGSLDAFTDAAKSAVMFDGQAYGYPKSVETPVLMYNKDL
MAEAPASMDDLYKLSNDVKADGQYGFLALWDNFYFAHGVVAGFGGYVFKEDGGALDPADIGLNNEGAVEGFEYIGKWYEE
GLFPKGLIGESGGQAMDQLFTEKKAHSVMNGPWAVAGYTDAGVNLGAAPMPTLPNGEPIKTFMGVKTYALSAYTENQEWA
EMFLQELTNEENALAMFEAYNEIPPVAALESNETITSNEVAKAVFDQATNAIPMPNIPEMGQVWEPMAQALQLVATGKQD
AQKSADDAVKVIEQQIQANNQ

Specific function: Binding protein for cyclodextrin; involved in its cellular uptake. Interacts with all natural cyclodextrins:alpha, beta and gamma [H]

COG id: COG2182

COG function: function code G; Maltose-binding periplasmic proteins/domains

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790466, Length=417, Percent_Identity=29.0167865707434, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006060
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45055; Mature: 45055

Theoretical pI: Translated: 4.04; Mature: 4.04

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKKMVAGLSTAVFAFGALAACGGGTDNGSSNEGGSSSENKPEKIVIWEDIEKSETTKE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
VAKAFEEEHGVKVEVVEVQMTDQKDKLALDGPAGKGPDIVLVPHDQIGTIADQGHLAPIA
HHHHHHHHCCCEEEEEEEEECCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DEGSLDAFTDAAKSAVMFDGQAYGYPKSVETPVLMYNKDLMAEAPASMDDLYKLSNDVKA
CCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHCCCCC
DGQYGFLALWDNFYFAHGVVAGFGGYVFKEDGGALDPADIGLNNEGAVEGFEYIGKWYEE
CCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
GLFPKGLIGESGGQAMDQLFTEKKAHSVMNGPWAVAGYTDAGVNLGAAPMPTLPNGEPIK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
TFMGVKTYALSAYTENQEWAEMFLQELTNEENALAMFEAYNEIPPVAALESNETITSNEV
HHHHHHHHEEEHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHH
AKAVFDQATNAIPMPNIPEMGQVWEPMAQALQLVATGKQDAQKSADDAVKVIEQQIQANN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
Q
C
>Mature Secondary Structure
MKMKKMVAGLSTAVFAFGALAACGGGTDNGSSNEGGSSSENKPEKIVIWEDIEKSETTKE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
VAKAFEEEHGVKVEVVEVQMTDQKDKLALDGPAGKGPDIVLVPHDQIGTIADQGHLAPIA
HHHHHHHHCCCEEEEEEEEECCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
DEGSLDAFTDAAKSAVMFDGQAYGYPKSVETPVLMYNKDLMAEAPASMDDLYKLSNDVKA
CCCCCHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCCHHHHHHHHCCCCC
DGQYGFLALWDNFYFAHGVVAGFGGYVFKEDGGALDPADIGLNNEGAVEGFEYIGKWYEE
CCCEEEEEEECCHHHHHHHHHHCCCEEEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
GLFPKGLIGESGGQAMDQLFTEKKAHSVMNGPWAVAGYTDAGVNLGAAPMPTLPNGEPIK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
TFMGVKTYALSAYTENQEWAEMFLQELTNEENALAMFEAYNEIPPVAALESNETITSNEV
HHHHHHHHEEEHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCHHHH
AKAVFDQATNAIPMPNIPEMGQVWEPMAQALQLVATGKQDAQKSADDAVKVIEQQIQANN
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]