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The map label for this gene is ygcA [C]

Identifier: 229918108

GI number: 229918108

Start: 2339755

End: 2341131

Strand: Direct

Name: ygcA [C]

Synonym: EAT1b_2388

Alternate gene names: 229918108

Gene position: 2339755-2341131 (Clockwise)

Preceding gene: 229918107

Following gene: 229918109

Centisome position: 77.99

GC content: 48.66

Gene sequence:

>1377_bases
TTGAAATTAATCACAGGAGATTTTAAAGATGTCACGGTCGAACGACTTGGCATTAATGGAGAAGGCATCGCGACGCTCAA
TCGCATGGTCATCTTCATTCCAAACTTATTGCCGGGAGAACGAGCCAAAGTCGAAATCACGCGTGTGGAAAAAAACTATG
CGGAAGCGCGTGTCGTCAAACGAGATAACGACTCGAAAGACCGCGTCAAAGCACCTTGTCCGATTTACGATGAGTGCGGG
GGCTGCCAAATCCAACATATGAGTTCACGTCTGCAGATGGAGTATAAGGAAGAAATCGTTCGAAACGCCTTCCGTCAAAA
GACAAAACTAAACGTGGACAAATCAGATATCCGTCCAACAATCGGAATGGAAGATCCATGGTATTACCGGAACAAGTCAC
AATTCCCACTCGGCACGCGTCGCGGTGAGATCGTATCAGGTCTCTACAAGCCGAACTCGAACCATCTCGTGCCGATCGAG
CACTGTATGGTCCAACAACGCGACACGACGAACATCAACAATGCGATTGTCCGTATTTTAAACGAACTTGATATTCCCGT
CTACGATGCACGCCGTGACACGGGGTACGCGCGTACCATCGTCGTCCGATCGGGGTATAACACGAACGATGTTCAAGTCG
TTTTGGTCGTCGGACATGAAGATGTCCCTGATTTAGATGAATTGAGTGACCGCATCATGGAATTGCCGAATGTTGTGTCG
TTCCATATCAACATCAATGCAAACCGTTCTGGTGTTATTTTCGGTTATCGGACGGTCCACATCGCTGGTCAGGAGAAAAT
GGATGAACGACTAGATGACGTCCACTATCATTTATCACCACGCGCATTCTTCCAGTTGAATCCGGCACAGACAGAAAAAA
TGTACCGTGAAGTGGTCGAGGCCGCAGGATTGACGGGTGAAGAACGTGTGATCGATGCCTATGCGGGAGTCGGGTCAATC
GGACTTTGGTTAGCCCCTTACGCGAAAGAAATCCGTGGAATGGAAGTCGTGCCGGAAGCGGTCGAAGATGCCAATGACCA
TATGCGTGAGAATGGCTATAACCATGTCACGTACGTCGAAGGACGTGCCGAGCATTGGATTCCGCGTTGGGTAAAAGATG
GATGGATTCCAGACGTCATCGTAGTCGACCCGCCACGCACAGGGATGGACCATCAATTGATCAATTCCATCATCTCTTCT
AAAACGAAGCGAATCGTTTACGTGTCATGTAACCCGCAGACGCTCGCACGTGACGCCGACCAGTTGATGAAAGCAGGATA
CAAAGTGAATTACGTACAGCCGTACGATATGTTCCCGCAGACGTCTCACGTCGAATCCGTCGTCGTCTTCGAGAAAAAGA
AAAAGAAAAAATATTGA

Upstream 100 bases:

>100_bases
AAGATTTACATCGCCTGTTAGCCGACATTGATTCGTTGCGCGCTAAAGTAGTGCGTTACGCCAAAGACCTAGAACAATCG
TCTCGAAAGGATGAATCAGA

Downstream 100 bases:

>100_bases
CGAGTCCGTGAAAAGACTTGTCGCGTAAGCGTTTTCACGCTATAGTTCAGATGTCAGACTTCTTTTCAAAAATAGAAGCG
GAGGAACATCCTATGATCTC

Product: RNA methyltransferase, TrmA family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKLITGDFKDVTVERLGINGEGIATLNRMVIFIPNLLPGERAKVEITRVEKNYAEARVVKRDNDSKDRVKAPCPIYDECG
GCQIQHMSSRLQMEYKEEIVRNAFRQKTKLNVDKSDIRPTIGMEDPWYYRNKSQFPLGTRRGEIVSGLYKPNSNHLVPIE
HCMVQQRDTTNINNAIVRILNELDIPVYDARRDTGYARTIVVRSGYNTNDVQVVLVVGHEDVPDLDELSDRIMELPNVVS
FHININANRSGVIFGYRTVHIAGQEKMDERLDDVHYHLSPRAFFQLNPAQTEKMYREVVEAAGLTGEERVIDAYAGVGSI
GLWLAPYAKEIRGMEVVPEAVEDANDHMRENGYNHVTYVEGRAEHWIPRWVKDGWIPDVIVVDPPRTGMDHQLINSIISS
KTKRIVYVSCNPQTLARDADQLMKAGYKVNYVQPYDMFPQTSHVESVVVFEKKKKKKY

Sequences:

>Translated_458_residues
MKLITGDFKDVTVERLGINGEGIATLNRMVIFIPNLLPGERAKVEITRVEKNYAEARVVKRDNDSKDRVKAPCPIYDECG
GCQIQHMSSRLQMEYKEEIVRNAFRQKTKLNVDKSDIRPTIGMEDPWYYRNKSQFPLGTRRGEIVSGLYKPNSNHLVPIE
HCMVQQRDTTNINNAIVRILNELDIPVYDARRDTGYARTIVVRSGYNTNDVQVVLVVGHEDVPDLDELSDRIMELPNVVS
FHININANRSGVIFGYRTVHIAGQEKMDERLDDVHYHLSPRAFFQLNPAQTEKMYREVVEAAGLTGEERVIDAYAGVGSI
GLWLAPYAKEIRGMEVVPEAVEDANDHMRENGYNHVTYVEGRAEHWIPRWVKDGWIPDVIVVDPPRTGMDHQLINSIISS
KTKRIVYVSCNPQTLARDADQLMKAGYKVNYVQPYDMFPQTSHVESVVVFEKKKKKKY
>Mature_458_residues
MKLITGDFKDVTVERLGINGEGIATLNRMVIFIPNLLPGERAKVEITRVEKNYAEARVVKRDNDSKDRVKAPCPIYDECG
GCQIQHMSSRLQMEYKEEIVRNAFRQKTKLNVDKSDIRPTIGMEDPWYYRNKSQFPLGTRRGEIVSGLYKPNSNHLVPIE
HCMVQQRDTTNINNAIVRILNELDIPVYDARRDTGYARTIVVRSGYNTNDVQVVLVVGHEDVPDLDELSDRIMELPNVVS
FHININANRSGVIFGYRTVHIAGQEKMDERLDDVHYHLSPRAFFQLNPAQTEKMYREVVEAAGLTGEERVIDAYAGVGSI
GLWLAPYAKEIRGMEVVPEAVEDANDHMRENGYNHVTYVEGRAEHWIPRWVKDGWIPDVIVVDPPRTGMDHQLINSIISS
KTKRIVYVSCNPQTLARDADQLMKAGYKVNYVQPYDMFPQTSHVESVVVFEKKKKKKY

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=357, Percent_Identity=27.7310924369748, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI51173878, Length=357, Percent_Identity=27.7310924369748, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI269784678, Length=401, Percent_Identity=27.1820448877805, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI269784680, Length=391, Percent_Identity=27.1099744245524, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI269784676, Length=391, Percent_Identity=27.1099744245524, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI20149688, Length=391, Percent_Identity=27.1099744245524, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789148, Length=458, Percent_Identity=30.5676855895196, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1787083, Length=353, Percent_Identity=25.2124645892351, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790403, Length=207, Percent_Identity=26.5700483091787, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71997438, Length=165, Percent_Identity=29.0909090909091, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24666579, Length=430, Percent_Identity=26.9767441860465, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52419; Mature: 52419

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITGDFKDVTVERLGINGEGIATLNRMVIFIPNLLPGERAKVEITRVEKNYAEARVVK
CCEECCCCCCEEEEEECCCCCCHHHHCEEEEEECCCCCCCCCEEEEEEEHHHHHHHEEEE
RDNDSKDRVKAPCPIYDECGGCQIQHMSSRLQMEYKEEIVRNAFRQKTKLNVDKSDIRPT
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
IGMEDPWYYRNKSQFPLGTRRGEIVSGLYKPNSNHLVPIEHCMVQQRDTTNINNAIVRIL
CCCCCCEEECCCCCCCCCCCCCHHHHHEECCCCCCEEEHHHHHHCCCCCCCHHHHHHHHH
NELDIPVYDARRDTGYARTIVVRSGYNTNDVQVVLVVGHEDVPDLDELSDRIMELPNVVS
HHCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEE
FHININANRSGVIFGYRTVHIAGQEKMDERLDDVHYHLSPRAFFQLNPAQTEKMYREVVE
EEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHEEECCCCEEEEECHHHHHHHHHHHHH
AAGLTGEERVIDAYAGVGSIGLWLAPYAKEIRGMEVVPEAVEDANDHMRENGYNHVTYVE
HHCCCCCHHHHHHHCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GRAEHWIPRWVKDGWIPDVIVVDPPRTGMDHQLINSIISSKTKRIVYVSCNPQTLARDAD
CCHHHCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
QLMKAGYKVNYVQPYDMFPQTSHVESVVVFEKKKKKKY
HHHHCCCEEEEECCCCCCCCCCCCCEEEEEEHHHCCCC
>Mature Secondary Structure
MKLITGDFKDVTVERLGINGEGIATLNRMVIFIPNLLPGERAKVEITRVEKNYAEARVVK
CCEECCCCCCEEEEEECCCCCCHHHHCEEEEEECCCCCCCCCEEEEEEEHHHHHHHEEEE
RDNDSKDRVKAPCPIYDECGGCQIQHMSSRLQMEYKEEIVRNAFRQKTKLNVDKSDIRPT
CCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
IGMEDPWYYRNKSQFPLGTRRGEIVSGLYKPNSNHLVPIEHCMVQQRDTTNINNAIVRIL
CCCCCCEEECCCCCCCCCCCCCHHHHHEECCCCCCEEEHHHHHHCCCCCCCHHHHHHHHH
NELDIPVYDARRDTGYARTIVVRSGYNTNDVQVVLVVGHEDVPDLDELSDRIMELPNVVS
HHCCCCEEECCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEE
FHININANRSGVIFGYRTVHIAGQEKMDERLDDVHYHLSPRAFFQLNPAQTEKMYREVVE
EEEEEECCCCEEEEEEEEEEECCHHHHHHHHHHHEEECCCCEEEEECHHHHHHHHHHHHH
AAGLTGEERVIDAYAGVGSIGLWLAPYAKEIRGMEVVPEAVEDANDHMRENGYNHVTYVE
HHCCCCCHHHHHHHCCCCCHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GRAEHWIPRWVKDGWIPDVIVVDPPRTGMDHQLINSIISSKTKRIVYVSCNPQTLARDAD
CCHHHCCHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
QLMKAGYKVNYVQPYDMFPQTSHVESVVVFEKKKKKKY
HHHHCCCEEEEECCCCCCCCCCCCCEEEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]