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The map label for this gene is bmr3 [H]

Identifier: 229918052

GI number: 229918052

Start: 2284118

End: 2285578

Strand: Direct

Name: bmr3 [H]

Synonym: EAT1b_2331

Alternate gene names: 229918052

Gene position: 2284118-2285578 (Clockwise)

Preceding gene: 229918051

Following gene: 229918053

Centisome position: 76.14

GC content: 49.97

Gene sequence:

>1461_bases
ATGAGAAAAAATGTGACGGTTGCCCTCTTACTGGCAACCTTTTTAGCGGCGATTGAAGGGACGGTCGTATCGGTTGCGAC
ACCGGTCATCGCCAGTGAGTTGAACGGGGCGACGCTCGTCAGCTGGGTATTTGCTGCCTTCTTACTCTTTACGGCGGTTT
CGACACCGATTTATGGAAAAGTGGCAGACTTATTCGGGCGAAAGCGCGTCCTGTTGTTTGGAATCGGGTTGTTCACCGTG
TCTTCATTGTTATGTGGGTTAGCGACATCGATGGAACAACTCATTGTCTTTCGGGCGCTGCAAGGTCTCGGGGCCGGAGC
CGTGCTTCCGATTTCGATGACGATTATTGGTGATTTATATAAATATGAAGAGCGTGGGAAAATTCAAGGAATTCTAAGTG
CGGTCTGGGGTGTATCGGGTGTGCTCGGTCCCGTCATCGGTGGATTTTTGGTCGAAACGTTGTCTTGGCGTTACGTATTC
TTATTAAACGTGCCATTTGCGTTACTCTCGTTCATCATGATTATCGTCTTTTACAAAGAAACCGTCGCTGAAACGAGCGA
GCGGATTGATTTGAAAGGTGCTCTCTTGTTCGCTGGAGGAACGACCGCGTTTTTATACGCCTTGATCACGTTCAGTGAAA
CGAATGAATGGACGACGACCATCGTCGGAAGTGGCGTACTTAGTCTTGTTTTGTTAGGGGGATTCTTCCTATCAGAAAGA
AGAGCAACCTCTCCGTTATTGCCGTTTGATTTGCTCAAGCAATCAACGATTGCGGCCATCAACGGCTCTGTCTTTTTTGC
GGCTTGGGTGCTCGTTTCGATGTCAGCTTACATCCCGATTTGGGCGCAAGCCGTGTTAGGAAAAACAGCGACCGAGGCTG
GATTCATGTTGATGCCGTTGTCTGTTGCATGGACATTCACATCGATTATCGGAGGACGGACGCTCGGTGTGGCCTCCCCG
AGACGTCGCGCGTTGGTTGGTATGGGGTTACTCGTCATCGGAACGTTCACGTTGACACTCTTATCTCAATCAAGCTCGGA
TTTCTGGGTGTATGGTGCGGTGGCTTTGATTGGGGTAGGGTTCGGATTGTCACAGCCGATGTTTATCGTCGTCTTGCAAA
CCGTCGTTTCATATCAGCAACGAGGAACGGCGACCGCGACCAATTCCTTCTTAAGTACGGTCGGTCAAACGCTCGGTGTA
GCAGTGTTTGGAGCTGTCTTTAACTTCGTCGTGCTCAACGACTTTAAGGAAAGTAGTGCGTTATCCCAAGTGTCTTTGGA
AGACTTCTTCAATCAAAGTGTGACGGAGACATTAAGCGCCTCTGTCCGTCTGCAAGGTGAACAGATTATTGCGACCGGAC
TAAACAGTATCTTCATCGGGGCGATGATTGCAGCCGTGATTGCATTTATCATCGCAACCCGTTTGCCGGCCCGCCCACCG
GAACCTTCACAGCGCTCGTAA

Upstream 100 bases:

>100_bases
TGACACTTGTCAAATGGACGCGGCTCGCACAAAATAATAGACAGTTGAAGTGGTGCTCGGAACCTTCCGACGCCACTATT
TTTTTGGGGAGATGGAATGA

Downstream 100 bases:

>100_bases
AACAAGGGTATGATGAAGGAAAGGGAGGCGAACCGACATGAAACGTCAAATCGGGATTGTGACACTATTGTTTCTTATGG
GATGTACGCCAGAACCGACG

Product: major facilitator superfamily MFS_1

Products: NA

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRKNVTVALLLATFLAAIEGTVVSVATPVIASELNGATLVSWVFAAFLLFTAVSTPIYGKVADLFGRKRVLLFGIGLFTV
SSLLCGLATSMEQLIVFRALQGLGAGAVLPISMTIIGDLYKYEERGKIQGILSAVWGVSGVLGPVIGGFLVETLSWRYVF
LLNVPFALLSFIMIIVFYKETVAETSERIDLKGALLFAGGTTAFLYALITFSETNEWTTTIVGSGVLSLVLLGGFFLSER
RATSPLLPFDLLKQSTIAAINGSVFFAAWVLVSMSAYIPIWAQAVLGKTATEAGFMLMPLSVAWTFTSIIGGRTLGVASP
RRRALVGMGLLVIGTFTLTLLSQSSSDFWVYGAVALIGVGFGLSQPMFIVVLQTVVSYQQRGTATATNSFLSTVGQTLGV
AVFGAVFNFVVLNDFKESSALSQVSLEDFFNQSVTETLSASVRLQGEQIIATGLNSIFIGAMIAAVIAFIIATRLPARPP
EPSQRS

Sequences:

>Translated_486_residues
MRKNVTVALLLATFLAAIEGTVVSVATPVIASELNGATLVSWVFAAFLLFTAVSTPIYGKVADLFGRKRVLLFGIGLFTV
SSLLCGLATSMEQLIVFRALQGLGAGAVLPISMTIIGDLYKYEERGKIQGILSAVWGVSGVLGPVIGGFLVETLSWRYVF
LLNVPFALLSFIMIIVFYKETVAETSERIDLKGALLFAGGTTAFLYALITFSETNEWTTTIVGSGVLSLVLLGGFFLSER
RATSPLLPFDLLKQSTIAAINGSVFFAAWVLVSMSAYIPIWAQAVLGKTATEAGFMLMPLSVAWTFTSIIGGRTLGVASP
RRRALVGMGLLVIGTFTLTLLSQSSSDFWVYGAVALIGVGFGLSQPMFIVVLQTVVSYQQRGTATATNSFLSTVGQTLGV
AVFGAVFNFVVLNDFKESSALSQVSLEDFFNQSVTETLSASVRLQGEQIIATGLNSIFIGAMIAAVIAFIIATRLPARPP
EPSQRS
>Mature_486_residues
MRKNVTVALLLATFLAAIEGTVVSVATPVIASELNGATLVSWVFAAFLLFTAVSTPIYGKVADLFGRKRVLLFGIGLFTV
SSLLCGLATSMEQLIVFRALQGLGAGAVLPISMTIIGDLYKYEERGKIQGILSAVWGVSGVLGPVIGGFLVETLSWRYVF
LLNVPFALLSFIMIIVFYKETVAETSERIDLKGALLFAGGTTAFLYALITFSETNEWTTTIVGSGVLSLVLLGGFFLSER
RATSPLLPFDLLKQSTIAAINGSVFFAAWVLVSMSAYIPIWAQAVLGKTATEAGFMLMPLSVAWTFTSIIGGRTLGVASP
RRRALVGMGLLVIGTFTLTLLSQSSSDFWVYGAVALIGVGFGLSQPMFIVVLQTVVSYQQRGTATATNSFLSTVGQTLGV
AVFGAVFNFVVLNDFKESSALSQVSLEDFFNQSVTETLSASVRLQGEQIIATGLNSIFIGAMIAAVIAFIIATRLPARPP
EPSQRS

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=436, Percent_Identity=27.9816513761468, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1790195, Length=416, Percent_Identity=28.3653846153846, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788710, Length=317, Percent_Identity=29.3375394321767, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789042, Length=329, Percent_Identity=28.2674772036474, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=290, Percent_Identity=29.3103448275862, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790146, Length=137, Percent_Identity=32.8467153284672, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788509, Length=166, Percent_Identity=31.9277108433735, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32564663, Length=164, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=322, Percent_Identity=28.2608695652174, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319770, Length=397, Percent_Identity=26.1964735516373, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322958, Length=490, Percent_Identity=24.0816326530612, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321663, Length=169, Percent_Identity=29.585798816568, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=494, Percent_Identity=24.0890688259109, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=395, Percent_Identity=22.2784810126582, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51729; Mature: 51729

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNVTVALLLATFLAAIEGTVVSVATPVIASELNGATLVSWVFAAFLLFTAVSTPIYGK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
VADLFGRKRVLLFGIGLFTVSSLLCGLATSMEQLIVFRALQGLGAGAVLPISMTIIGDLY
HHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KYEERGKIQGILSAVWGVSGVLGPVIGGFLVETLSWRYVFLLNVPFALLSFIMIIVFYKE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
TVAETSERIDLKGALLFAGGTTAFLYALITFSETNEWTTTIVGSGVLSLVLLGGFFLSER
HHHHHHHHCEECEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
RATSPLLPFDLLKQSTIAAINGSVFFAAWVLVSMSAYIPIWAQAVLGKTATEAGFMLMPL
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEHH
SVAWTFTSIIGGRTLGVASPRRRALVGMGLLVIGTFTLTLLSQSSSDFWVYGAVALIGVG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHC
FGLSQPMFIVVLQTVVSYQQRGTATATNSFLSTVGQTLGVAVFGAVFNFVVLNDFKESSA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LSQVSLEDFFNQSVTETLSASVRLQGEQIIATGLNSIFIGAMIAAVIAFIIATRLPARPP
HHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPSQRS
CCCCCC
>Mature Secondary Structure
MRKNVTVALLLATFLAAIEGTVVSVATPVIASELNGATLVSWVFAAFLLFTAVSTPIYGK
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
VADLFGRKRVLLFGIGLFTVSSLLCGLATSMEQLIVFRALQGLGAGAVLPISMTIIGDLY
HHHHHCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KYEERGKIQGILSAVWGVSGVLGPVIGGFLVETLSWRYVFLLNVPFALLSFIMIIVFYKE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHH
TVAETSERIDLKGALLFAGGTTAFLYALITFSETNEWTTTIVGSGVLSLVLLGGFFLSER
HHHHHHHHCEECEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
RATSPLLPFDLLKQSTIAAINGSVFFAAWVLVSMSAYIPIWAQAVLGKTATEAGFMLMPL
CCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEEEHH
SVAWTFTSIIGGRTLGVASPRRRALVGMGLLVIGTFTLTLLSQSSSDFWVYGAVALIGVG
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHC
FGLSQPMFIVVLQTVVSYQQRGTATATNSFLSTVGQTLGVAVFGAVFNFVVLNDFKESSA
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LSQVSLEDFFNQSVTETLSASVRLQGEQIIATGLNSIFIGAMIAAVIAFIIATRLPARPP
HHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
EPSQRS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]