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The map label for this gene is yaaJ [C]

Identifier: 229918018

GI number: 229918018

Start: 2252143

End: 2253495

Strand: Direct

Name: yaaJ [C]

Synonym: EAT1b_2297

Alternate gene names: 229918018

Gene position: 2252143-2253495 (Clockwise)

Preceding gene: 229918017

Following gene: 229918020

Centisome position: 75.07

GC content: 51.15

Gene sequence:

>1353_bases
ATGGGGCAATCATTTAGTGAATGGGTGAGTAAGGCATCCAACTTTGTATGGGGACCACCGCTCTTGATTTTGCTTGTGGG
GACAGGCATTTACTTGACGGTGCGTCTAGGTGGGATTCAAATCACCAAATTACCGTATGCGTTAAAATTGGCGTTTTCGA
AAAATCAGGATCAATCTTCTAAAGGGGATATCAGTCACTTCCAGGCGCTCATGACCGCTCTGGCGGCGACCGTCGGTACG
GGGAATATCGTCGGAGTGTCGACAGCGGTCGTATTAGGAGGACCAGGAGCCATCGTCTGGATGTGGGTGTCCGGTTTCTT
TGGGATGGCGACGAAATACGCGGAGGCGGTTTTGGCCGTGAAGTATCGGGTCACCGATGAGCGCGGTCAGATGGCTGGTG
GACCGATGTACTATCTAGAACGAGGATTGAATCAACGATGGTTAGCAGTCGCATTTGCGGCATTCGCATCGATTGCGGCG
TTCGGTATTGGAAACGGGGTCCAAACGAACTCGGTCGCATTGGCCTTAAAAACGACATTCGATGTACCACTCTACATATC
AGGGATTCTCATTATGATTTTTACAGGTGCGGTCATCCTTGGAGGCGTTCGTTCCATCGGGAAAGTGGTCAGTTACTTCG
TCCCCGTCATGATTGCCTTCTACTTAGGTAGCGGTCTCATCATTATGGTGATGAATTACTCACTGATTCCGGATGCGTTC
GCACTCATCTTTAGCAGTACGTTCAGTGCAGAAGCAATCGGTGGAGGGGCCATCGGAGCTGCCATTCGATACGGGGTGGC
GCGTGGTGTCTTCTCGAACGAAGCGGGTCTCGGCTCTGCTCCGATTGCAGCAGCGGCAGCGAAAACAGATATGCCGGGTC
GTCAAGCGCTCGTCTCGATGACTCAAGTCTTCTTGGACACGCTTGTCGTCTGTTCGGTGACAGGGTTGACACTCGTCATG
GGTGGTCAAATTGGGACAGGACTAGAAGGAGTCGAATTGACCTCAGCTACATTTGAACTGTTCCTCGGACCGGCCGGCGG
TTATATTGTCACGATTGGGCTTGTCCTGTTCGCTTATTCGACTGTGCTCGGTTGGTCGTATTACGGTGAGCGATCCATCC
ATTACTTGTTCGGACAAAAGTCGATCCTTCCGTATCGGATTGCTTTTGTACTCGTTGCGGGACTCGGTGCCATGACGACG
AACTTGAATTTGGTATGGGCGCTATCGGATGTCTTTAACGGCTTGATGGCCATCCCGAACTTGATCGGTCTCTTGTTCTT
ATCGGGTGTTGTCATCGCCGAGACGAAGCGTTTCAACCAACAGCTTGAAATTGAAAATCGGAAAGTTTCCTAA

Upstream 100 bases:

>100_bases
AAAATGGATTGGTTGTTTTAGTACAATACTTTTGCATTGTTCTAAGTATTCTGAAAAAATAGAGGTGTACACTATTTTTC
GGGGAAAGGGGATACGTTGT

Downstream 100 bases:

>100_bases
TCGTAAGGACGCCATCCTCATGAATCAGAGGGTGGCGTCTTTTGTTTCGCTTCCAATTCATCGAGTCGTGCTTTTTGTAA
ATGGTCAAATACTTTTGTGA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MGQSFSEWVSKASNFVWGPPLLILLVGTGIYLTVRLGGIQITKLPYALKLAFSKNQDQSSKGDISHFQALMTALAATVGT
GNIVGVSTAVVLGGPGAIVWMWVSGFFGMATKYAEAVLAVKYRVTDERGQMAGGPMYYLERGLNQRWLAVAFAAFASIAA
FGIGNGVQTNSVALALKTTFDVPLYISGILIMIFTGAVILGGVRSIGKVVSYFVPVMIAFYLGSGLIIMVMNYSLIPDAF
ALIFSSTFSAEAIGGGAIGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDMPGRQALVSMTQVFLDTLVVCSVTGLTLVM
GGQIGTGLEGVELTSATFELFLGPAGGYIVTIGLVLFAYSTVLGWSYYGERSIHYLFGQKSILPYRIAFVLVAGLGAMTT
NLNLVWALSDVFNGLMAIPNLIGLLFLSGVVIAETKRFNQQLEIENRKVS

Sequences:

>Translated_450_residues
MGQSFSEWVSKASNFVWGPPLLILLVGTGIYLTVRLGGIQITKLPYALKLAFSKNQDQSSKGDISHFQALMTALAATVGT
GNIVGVSTAVVLGGPGAIVWMWVSGFFGMATKYAEAVLAVKYRVTDERGQMAGGPMYYLERGLNQRWLAVAFAAFASIAA
FGIGNGVQTNSVALALKTTFDVPLYISGILIMIFTGAVILGGVRSIGKVVSYFVPVMIAFYLGSGLIIMVMNYSLIPDAF
ALIFSSTFSAEAIGGGAIGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDMPGRQALVSMTQVFLDTLVVCSVTGLTLVM
GGQIGTGLEGVELTSATFELFLGPAGGYIVTIGLVLFAYSTVLGWSYYGERSIHYLFGQKSILPYRIAFVLVAGLGAMTT
NLNLVWALSDVFNGLMAIPNLIGLLFLSGVVIAETKRFNQQLEIENRKVS
>Mature_449_residues
GQSFSEWVSKASNFVWGPPLLILLVGTGIYLTVRLGGIQITKLPYALKLAFSKNQDQSSKGDISHFQALMTALAATVGTG
NIVGVSTAVVLGGPGAIVWMWVSGFFGMATKYAEAVLAVKYRVTDERGQMAGGPMYYLERGLNQRWLAVAFAAFASIAAF
GIGNGVQTNSVALALKTTFDVPLYISGILIMIFTGAVILGGVRSIGKVVSYFVPVMIAFYLGSGLIIMVMNYSLIPDAFA
LIFSSTFSAEAIGGGAIGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDMPGRQALVSMTQVFLDTLVVCSVTGLTLVMG
GQIGTGLEGVELTSATFELFLGPAGGYIVTIGLVLFAYSTVLGWSYYGERSIHYLFGQKSILPYRIAFVLVAGLGAMTTN
LNLVWALSDVFNGLMAIPNLIGLLFLSGVVIAETKRFNQQLEIENRKVS

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=454, Percent_Identity=37.2246696035242, Blast_Score=261, Evalue=5e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47463; Mature: 47332

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSFSEWVSKASNFVWGPPLLILLVGTGIYLTVRLGGIQITKLPYALKLAFSKNQDQSS
CCCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCEEEEECCEEEEEEECCCCCCCC
KGDISHFQALMTALAATVGTGNIVGVSTAVVLGGPGAIVWMWVSGFFGMATKYAEAVLAV
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHE
KYRVTDERGQMAGGPMYYLERGLNQRWLAVAFAAFASIAAFGIGNGVQTNSVALALKTTF
EEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
DVPLYISGILIMIFTGAVILGGVRSIGKVVSYFVPVMIAFYLGSGLIIMVMNYSLIPDAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
ALIFSSTFSAEAIGGGAIGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDMPGRQALVSM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHH
TQVFLDTLVVCSVTGLTLVMGGQIGTGLEGVELTSATFELFLGPAGGYIVTIGLVLFAYS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEHHHEEEEEECCCCCHHHHHHHHHHHHH
TVLGWSYYGERSIHYLFGQKSILPYRIAFVLVAGLGAMTTNLNLVWALSDVFNGLMAIPN
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCEEEEEHHHHHHHHHHHHH
LIGLLFLSGVVIAETKRFNQQLEIENRKVS
HHHHHHHCCHHEEHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
GQSFSEWVSKASNFVWGPPLLILLVGTGIYLTVRLGGIQITKLPYALKLAFSKNQDQSS
CCCHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEEECCEEEEECCEEEEEEECCCCCCCC
KGDISHFQALMTALAATVGTGNIVGVSTAVVLGGPGAIVWMWVSGFFGMATKYAEAVLAV
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHE
KYRVTDERGQMAGGPMYYLERGLNQRWLAVAFAAFASIAAFGIGNGVQTNSVALALKTTF
EEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
DVPLYISGILIMIFTGAVILGGVRSIGKVVSYFVPVMIAFYLGSGLIIMVMNYSLIPDAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHH
ALIFSSTFSAEAIGGGAIGAAIRYGVARGVFSNEAGLGSAPIAAAAAKTDMPGRQALVSM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHH
TQVFLDTLVVCSVTGLTLVMGGQIGTGLEGVELTSATFELFLGPAGGYIVTIGLVLFAYS
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEHHHEEEEEECCCCCHHHHHHHHHHHHH
TVLGWSYYGERSIHYLFGQKSILPYRIAFVLVAGLGAMTTNLNLVWALSDVFNGLMAIPN
HHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHCCCEEEEEHHHHHHHHHHHHH
LIGLLFLSGVVIAETKRFNQQLEIENRKVS
HHHHHHHCCHHEEHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]