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The map label for this gene is glsA

Identifier: 229917972

GI number: 229917972

Start: 2205041

End: 2205982

Strand: Direct

Name: glsA

Synonym: EAT1b_2251

Alternate gene names: 229917972

Gene position: 2205041-2205982 (Clockwise)

Preceding gene: 229917971

Following gene: 229917974

Centisome position: 73.5

GC content: 49.89

Gene sequence:

>942_bases
ATGAATGTACTCGAACAACAGTTGAACCACTTGGTTGATGAGTGTCGTCCGTACACGGCTCAAGGGAAAGTGGCGGACTA
TATCCCTGAACTCGCACATGTGAAAAACGATTTACTCGGGGTGGCGATTTGTTTGCCGGATGGTACGTATATTCACGCGG
GGGATGTTCATCACAAGTTTACGATTCAGAGCGTATCGAAGGCGCTCACACTTTGTTATGTATTGATGGAGTTCGGGGAG
GACTATGTCTTTTCGAAAGTAGGGATGGAGCCGACGGGGGACGCATTCAACTCCATCGCCAAACTCGAGGAAACGGTCCC
GTCAAAACCACTGAATCCGATGATCAACGCCGGAGCGTTAGCGGTGACGAGTATGATTTTGGGGGAGACGGCAGAACTCA
AAATCTATCAGTTCCGCCAGTTTTTAGCGACGTTGTTAAATCGCTCGGTCGAGGAGGTCACGTACGACGAAAAAGTCGCC
CGCTCTGAATACGAGACGACCGACTTGAACCGTGCCTTGCTCTATTTCATGCGTCACCATGGAATTGTGGAAGGTGACGT
GGATGAGATCATTGATGTGTATACGAAACAGTGTGCCATTGAGATTGATTGTTTTGATTTGGCACGAATCGGGCGTGTCT
TTGCAGGGAATGGAGAAGACCCGGATACGGGCGAAGAGTTGATTCCACGCCGGGTCGTCCGAATCGTCAAGGCCATCATG
ACGACGTGTGGCATGTATGATGCATCGGGTGAGTTCGCTGTCCGGGTTGGTCTTCCAGGAAAAAGTGGGGTGTCGGGTGC
CATCTTGGCGGTCGGGAACCACGAACTAGATTTAAATAACGTCGGGTTTGGCATATTTGGTCCGGCACTTGATTCAAAAG
GAAACTCGATTGCCGGAATGAAGTTGTTGGAACTATTGATTGAGCGCTATACTTCTCGCTAA

Upstream 100 bases:

>100_bases
GATTTGGGCGGATGAAGACTGGTATAAAGATGATGGACCACCGGACGGATAATATGATAAGATGACCCGGTTGAAATTTG
ATGGAACATGAGGTGATGGA

Downstream 100 bases:

>100_bases
ATCGATAGGTGCTCCACGGATGCATCGAGTTCCTGCTGTAATGTTTCAATTTTTCGGTCGTATGCGGACTGTAGTTCAGC
TGGAGGGGTGAAGGCCCGCC

Product: Glutaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNVLEQQLNHLVDECRPYTAQGKVADYIPELAHVKNDLLGVAICLPDGTYIHAGDVHHKFTIQSVSKALTLCYVLMEFGE
DYVFSKVGMEPTGDAFNSIAKLEETVPSKPLNPMINAGALAVTSMILGETAELKIYQFRQFLATLLNRSVEEVTYDEKVA
RSEYETTDLNRALLYFMRHHGIVEGDVDEIIDVYTKQCAIEIDCFDLARIGRVFAGNGEDPDTGEELIPRRVVRIVKAIM
TTCGMYDASGEFAVRVGLPGKSGVSGAILAVGNHELDLNNVGFGIFGPALDSKGNSIAGMKLLELLIERYTSR

Sequences:

>Translated_313_residues
MNVLEQQLNHLVDECRPYTAQGKVADYIPELAHVKNDLLGVAICLPDGTYIHAGDVHHKFTIQSVSKALTLCYVLMEFGE
DYVFSKVGMEPTGDAFNSIAKLEETVPSKPLNPMINAGALAVTSMILGETAELKIYQFRQFLATLLNRSVEEVTYDEKVA
RSEYETTDLNRALLYFMRHHGIVEGDVDEIIDVYTKQCAIEIDCFDLARIGRVFAGNGEDPDTGEELIPRRVVRIVKAIM
TTCGMYDASGEFAVRVGLPGKSGVSGAILAVGNHELDLNNVGFGIFGPALDSKGNSIAGMKLLELLIERYTSR
>Mature_313_residues
MNVLEQQLNHLVDECRPYTAQGKVADYIPELAHVKNDLLGVAICLPDGTYIHAGDVHHKFTIQSVSKALTLCYVLMEFGE
DYVFSKVGMEPTGDAFNSIAKLEETVPSKPLNPMINAGALAVTSMILGETAELKIYQFRQFLATLLNRSVEEVTYDEKVA
RSEYETTDLNRALLYFMRHHGIVEGDVDEIIDVYTKQCAIEIDCFDLARIGRVFAGNGEDPDTGEELIPRRVVRIVKAIM
TTCGMYDASGEFAVRVGLPGKSGVSGAILAVGNHELDLNNVGFGIFGPALDSKGNSIAGMKLLELLIERYTSR

Specific function: Unknown

COG id: COG2066

COG function: function code E; Glutaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutaminase family

Homologues:

Organism=Homo sapiens, GI156104878, Length=289, Percent_Identity=38.7543252595156, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI20336214, Length=293, Percent_Identity=36.1774744027304, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787804, Length=304, Percent_Identity=38.8157894736842, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1786693, Length=282, Percent_Identity=39.0070921985816, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17532727, Length=309, Percent_Identity=37.8640776699029, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17507019, Length=298, Percent_Identity=36.5771812080537, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI193204073, Length=318, Percent_Identity=34.5911949685535, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI193204075, Length=320, Percent_Identity=34.0625, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI281363241, Length=313, Percent_Identity=35.7827476038339, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI281363239, Length=313, Percent_Identity=35.7827476038339, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653162, Length=311, Percent_Identity=35.3697749196142, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653158, Length=313, Percent_Identity=35.7827476038339, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653164, Length=311, Percent_Identity=35.3697749196142, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653156, Length=313, Percent_Identity=35.7827476038339, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI116008307, Length=311, Percent_Identity=35.3697749196142, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653166, Length=311, Percent_Identity=35.3697749196142, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLSA_EXISA (C4L2C1)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886618.1
- ProteinModelPortal:   C4L2C1
- GeneID:   7870838
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2251
- OMA:   RNASIAY
- ProtClustDB:   CLSK2488972
- HAMAP:   MF_00313
- InterPro:   IPR012338
- InterPro:   IPR015868
- Gene3D:   G3DSA:3.40.710.20
- PANTHER:   PTHR12544
- TIGRFAMs:   TIGR03814

Pfam domain/function: PF04960 Glutaminase; SSF56601 PBP_transp_fold

EC number: =3.5.1.2

Molecular weight: Translated: 34413; Mature: 34413

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLEQQLNHLVDECRPYTAQGKVADYIPELAHVKNDLLGVAICLPDGTYIHAGDVHHKF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCCHHH
TIQSVSKALTLCYVLMEFGEDYVFSKVGMEPTGDAFNSIAKLEETVPSKPLNPMINAGAL
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHH
AVTSMILGETAELKIYQFRQFLATLLNRSVEEVTYDEKVARSEYETTDLNRALLYFMRHH
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIVEGDVDEIIDVYTKQCAIEIDCFDLARIGRVFAGNGEDPDTGEELIPRRVVRIVKAIM
CCCCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TTCGMYDASGEFAVRVGLPGKSGVSGAILAVGNHELDLNNVGFGIFGPALDSKGNSIAGM
HHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEECCCCCCEEECCCCCCCCCCHHHH
KLLELLIERYTSR
HHHHHHHHHHHCC
>Mature Secondary Structure
MNVLEQQLNHLVDECRPYTAQGKVADYIPELAHVKNDLLGVAICLPDGTYIHAGDVHHKF
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCEEECCCCCHHH
TIQSVSKALTLCYVLMEFGEDYVFSKVGMEPTGDAFNSIAKLEETVPSKPLNPMINAGAL
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHH
AVTSMILGETAELKIYQFRQFLATLLNRSVEEVTYDEKVARSEYETTDLNRALLYFMRHH
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIVEGDVDEIIDVYTKQCAIEIDCFDLARIGRVFAGNGEDPDTGEELIPRRVVRIVKAIM
CCCCCCHHHHHHHHHHHHEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
TTCGMYDASGEFAVRVGLPGKSGVSGAILAVGNHELDLNNVGFGIFGPALDSKGNSIAGM
HHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCEEECCCCCCEEECCCCCCCCCCHHHH
KLLELLIERYTSR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA