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The map label for this gene is pcrA [H]

Identifier: 229917962

GI number: 229917962

Start: 2190532

End: 2192748

Strand: Direct

Name: pcrA [H]

Synonym: EAT1b_2241

Alternate gene names: 229917962

Gene position: 2190532-2192748 (Clockwise)

Preceding gene: 229917961

Following gene: 229917963

Centisome position: 73.02

GC content: 50.74

Gene sequence:

>2217_bases
ATGTATAATCTTAGCGAAGAATTAGTCAAAGGCATGAACCCGGAACAAGCAGAAGCGGTGAAGCATACGGATGGCCCGCT
GTTGATCATGGCGGGTGCAGGGTCTGGGAAGACTCGTGTCTTGACCCATCGTGTCGCCTATTTGATGGCGGCGAAGCAAG
TGGCACCGTGGAACATTTTAGCGATCACGTTCACGAATAAGGCAGCACGAGAAATGAAAGATCGGATTGCCCGTCTTGTG
GGTGGTGTCGCCGAAGACATTTGGATTTCTACATTCCACTCCATGTGCGTCCGGATTTTACGACGCGATATCGATCGCAT
CCGCTATGACAGGAGCTTTTCCATTTTAGATGCCTCGGATCAATTGACAGCAATCAAACAGGTGTTGAAAGAATTGAACC
TCGATCCGAAGAAGTACGAACCGCGTACCCTTCTCGGGATGATTTCAAATCATAAAAACGAACTGCGCACGGCGAAGGAT
GCGGCCGCCCTCGTCGGGAACAATCCGTACGAGCGAATCATCTCGGACGTCTATACGGCGTATGAGAAAAAGCTGAAACA
AAACAATGTGCTCGACTTTGACGACTTGATCATGAAGACGATTCACTTATTCCAAGAAGCACCGGACGTGTTGGCGTTCT
ATCAGAAGAAATTCAAGTACATCCATGTCGATGAGTATCAGGATACGAACCGTGCCCAATATACGCTCGTCAAACTTCTC
GCACAGGCACATGAGAATTTGTGTGTCGTCGGGGATTCGGATCAGTCCATTTATCGTTGGCGCGGTGCCGACATCGCCAA
CATCTTGACGTTCGAGAAAGATTATCCGAGCGCCCATGTCATCTTGCTCGAACAAAACTATCGTTCGACGAAACGGATTT
TAGAGGCGGCCAACGGCGTCATCCAAAACAACGCTTCCCGGAAAGAGAAGAACTTGTGGACTGAAAATGACGAAGGGGAG
AAATTGTTGCTCCATGTCGCATCAGACGATCGCGACGAAGCGTTCTTCATCATGAACCAGATGAAAGAGCTGAAGATGGA
AGGGATGGACTACGGACAGATGGCGGTCCTTTACCGGACGAACGCACAATCACGTGGACTTGAGGAGATGCTCCTCAAAT
CGAACATCCCTTACAAGATGGTCGGTGGGACGAAGTTCTATGAGCGAAAAGAGATCAAGGACGTCCTCGCATACTTGCGA
TTGATCGCCAACCCGGATGAGGACATCTCGTTCGTTCGCGTCGTCAATGAGCCGAAACGTGGGATTGGTGCTGCGACGAT
TGATAAACTAGGGGATTTTGCAGACATGCAGGGCGTCTCGCTCATGGATGCGATTCGTGACATCGAATTGTCAGGCATCG
CCCCACGCACTGCGACGAAACTTGCGGATTTCCGTCAGATGATGATGGATTTACGACAGATGGCGGACTACCTCTCCATT
TCAGAACTCGTTGAGGAAGTGTTGAAGAAGACAGGTTATGAAGAGATGCTCAAAATCGAGAAGACGCTTGAGGCGGAAAG
CCGACTTGAGAACTTGCAAGAGTTCTTGTCGGTCGCCCAAAACTTTGAGAAAGAGAGCGATGAACAGACGCTCGTCGCCT
TCCTCACGGATTTGACGCTCGTCTCAGACCTCGATTCACTTGATGAGGTCGATGAGTCACATCAAGTCACCCTGATGACG
TTGCACTCGGCGAAGGGACTTGAATTCCCGGTCGTCTTCCTGATTGGAATGGAAGAAGGGCTCTTCCCGCACAGCCGTGC
CTTGAACGACGAGGAAGAGATGGAAGAAGAACGTCGTCTCGCGTATGTCGGGATCACCCGTGCCGAGAAGCGTCTCTATT
TGACACGTGCTCAGTCACGAATGCTATACGGTCGCTTCCAAAACAACCCAGAGTCGAGATTCTTGCATGAGTTGCCGGAA
ACGTTGTTAGAGCGGTCCGGTAAGGCGAGAAAACCGATGCCATGGAACCCGGTCGAGTCACCAGGGAAGTTACCAGTGAA
TGGCTTTAGTTCGAAACCGAAGCCGAAACTCGCCCAATCATCCGGTGCCGAAACGGTCGGCTGGAATGTCGGCGACAAAG
CGAATCATAAAAAATGGGGACAAGGGACGGTCGTACAGACACGTGGTGAAGGCGACCAACTCGAAGTCGACATCGCATTC
CCGGAAGTCGGGATCAAGCGCCTATTGGCTAAATTTGCCCCTATCGAAAAAGCGTGA

Upstream 100 bases:

>100_bases
TCGGCAATATCATTTATGAAGACTTAGCTGGAGCACTTGCGACTGTCCGAGCACTCCGATAAAATAAGAACAAACGTTTG
GAACAAGGAAGGAAACGATT

Downstream 100 bases:

>100_bases
GGTGAGAATATGGAGATGGCAGAACGCGTAGAAGAGCTACGTCAAACGTTAAATCAATATGGTTACGAGTATTACGTACT
CGACCGTCCGAGCGTGCCGG

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 738; Mature: 738

Protein sequence:

>738_residues
MYNLSEELVKGMNPEQAEAVKHTDGPLLIMAGAGSGKTRVLTHRVAYLMAAKQVAPWNILAITFTNKAAREMKDRIARLV
GGVAEDIWISTFHSMCVRILRRDIDRIRYDRSFSILDASDQLTAIKQVLKELNLDPKKYEPRTLLGMISNHKNELRTAKD
AAALVGNNPYERIISDVYTAYEKKLKQNNVLDFDDLIMKTIHLFQEAPDVLAFYQKKFKYIHVDEYQDTNRAQYTLVKLL
AQAHENLCVVGDSDQSIYRWRGADIANILTFEKDYPSAHVILLEQNYRSTKRILEAANGVIQNNASRKEKNLWTENDEGE
KLLLHVASDDRDEAFFIMNQMKELKMEGMDYGQMAVLYRTNAQSRGLEEMLLKSNIPYKMVGGTKFYERKEIKDVLAYLR
LIANPDEDISFVRVVNEPKRGIGAATIDKLGDFADMQGVSLMDAIRDIELSGIAPRTATKLADFRQMMMDLRQMADYLSI
SELVEEVLKKTGYEEMLKIEKTLEAESRLENLQEFLSVAQNFEKESDEQTLVAFLTDLTLVSDLDSLDEVDESHQVTLMT
LHSAKGLEFPVVFLIGMEEGLFPHSRALNDEEEMEEERRLAYVGITRAEKRLYLTRAQSRMLYGRFQNNPESRFLHELPE
TLLERSGKARKPMPWNPVESPGKLPVNGFSSKPKPKLAQSSGAETVGWNVGDKANHKKWGQGTVVQTRGEGDQLEVDIAF
PEVGIKRLLAKFAPIEKA

Sequences:

>Translated_738_residues
MYNLSEELVKGMNPEQAEAVKHTDGPLLIMAGAGSGKTRVLTHRVAYLMAAKQVAPWNILAITFTNKAAREMKDRIARLV
GGVAEDIWISTFHSMCVRILRRDIDRIRYDRSFSILDASDQLTAIKQVLKELNLDPKKYEPRTLLGMISNHKNELRTAKD
AAALVGNNPYERIISDVYTAYEKKLKQNNVLDFDDLIMKTIHLFQEAPDVLAFYQKKFKYIHVDEYQDTNRAQYTLVKLL
AQAHENLCVVGDSDQSIYRWRGADIANILTFEKDYPSAHVILLEQNYRSTKRILEAANGVIQNNASRKEKNLWTENDEGE
KLLLHVASDDRDEAFFIMNQMKELKMEGMDYGQMAVLYRTNAQSRGLEEMLLKSNIPYKMVGGTKFYERKEIKDVLAYLR
LIANPDEDISFVRVVNEPKRGIGAATIDKLGDFADMQGVSLMDAIRDIELSGIAPRTATKLADFRQMMMDLRQMADYLSI
SELVEEVLKKTGYEEMLKIEKTLEAESRLENLQEFLSVAQNFEKESDEQTLVAFLTDLTLVSDLDSLDEVDESHQVTLMT
LHSAKGLEFPVVFLIGMEEGLFPHSRALNDEEEMEEERRLAYVGITRAEKRLYLTRAQSRMLYGRFQNNPESRFLHELPE
TLLERSGKARKPMPWNPVESPGKLPVNGFSSKPKPKLAQSSGAETVGWNVGDKANHKKWGQGTVVQTRGEGDQLEVDIAF
PEVGIKRLLAKFAPIEKA
>Mature_738_residues
MYNLSEELVKGMNPEQAEAVKHTDGPLLIMAGAGSGKTRVLTHRVAYLMAAKQVAPWNILAITFTNKAAREMKDRIARLV
GGVAEDIWISTFHSMCVRILRRDIDRIRYDRSFSILDASDQLTAIKQVLKELNLDPKKYEPRTLLGMISNHKNELRTAKD
AAALVGNNPYERIISDVYTAYEKKLKQNNVLDFDDLIMKTIHLFQEAPDVLAFYQKKFKYIHVDEYQDTNRAQYTLVKLL
AQAHENLCVVGDSDQSIYRWRGADIANILTFEKDYPSAHVILLEQNYRSTKRILEAANGVIQNNASRKEKNLWTENDEGE
KLLLHVASDDRDEAFFIMNQMKELKMEGMDYGQMAVLYRTNAQSRGLEEMLLKSNIPYKMVGGTKFYERKEIKDVLAYLR
LIANPDEDISFVRVVNEPKRGIGAATIDKLGDFADMQGVSLMDAIRDIELSGIAPRTATKLADFRQMMMDLRQMADYLSI
SELVEEVLKKTGYEEMLKIEKTLEAESRLENLQEFLSVAQNFEKESDEQTLVAFLTDLTLVSDLDSLDEVDESHQVTLMT
LHSAKGLEFPVVFLIGMEEGLFPHSRALNDEEEMEEERRLAYVGITRAEKRLYLTRAQSRMLYGRFQNNPESRFLHELPE
TLLERSGKARKPMPWNPVESPGKLPVNGFSSKPKPKLAQSSGAETVGWNVGDKANHKKWGQGTVVQTRGEGDQLEVDIAF
PEVGIKRLLAKFAPIEKA

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=738, Percent_Identity=40.5149051490515, Blast_Score=546, Evalue=1e-156,
Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=42.3197492163009, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787196, Length=338, Percent_Identity=28.1065088757396, Blast_Score=113, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=731, Percent_Identity=29.0013679890561, Blast_Score=224, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6324477, Length=694, Percent_Identity=23.342939481268, Blast_Score=124, Evalue=4e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84008; Mature: 84008

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLSEELVKGMNPEQAEAVKHTDGPLLIMAGAGSGKTRVLTHRVAYLMAAKQVAPWNIL
CCCCHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEE
AITFTNKAAREMKDRIARLVGGVAEDIWISTFHSMCVRILRRDIDRIRYDRSFSILDASD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
QLTAIKQVLKELNLDPKKYEPRTLLGMISNHKNELRTAKDAAALVGNNPYERIISDVYTA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YEKKLKQNNVLDFDDLIMKTIHLFQEAPDVLAFYQKKFKYIHVDEYQDTNRAQYTLVKLL
HHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
AQAHENLCVVGDSDQSIYRWRGADIANILTFEKDYPSAHVILLEQNYRSTKRILEAANGV
HHHCCCEEEECCCCCCEEEECCCCHHHHHEECCCCCCEEEEEEECCCHHHHHHHHHHCHH
IQNNASRKEKNLWTENDEGEKLLLHVASDDRDEAFFIMNQMKELKMEGMDYGQMAVLYRT
HHCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NAQSRGLEEMLLKSNIPYKMVGGTKFYERKEIKDVLAYLRLIANPDEDISFVRVVNEPKR
CCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GIGAATIDKLGDFADMQGVSLMDAIRDIELSGIAPRTATKLADFRQMMMDLRQMADYLSI
CCCHHHHHHHCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SELVEEVLKKTGYEEMLKIEKTLEAESRLENLQEFLSVAQNFEKESDEQTLVAFLTDLTL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSDLDSLDEVDESHQVTLMTLHSAKGLEFPVVFLIGMEEGLFPHSRALNDEEEMEEERRL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHHHCE
AYVGITRAEKRLYLTRAQSRMLYGRFQNNPESRFLHELPETLLERSGKARKPMPWNPVES
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGKLPVNGFSSKPKPKLAQSSGAETVGWNVGDKANHKKWGQGTVVQTRGEGDQLEVDIAF
CCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEC
PEVGIKRLLAKFAPIEKA
CHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MYNLSEELVKGMNPEQAEAVKHTDGPLLIMAGAGSGKTRVLTHRVAYLMAAKQVAPWNIL
CCCCHHHHHCCCCCCHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEE
AITFTNKAAREMKDRIARLVGGVAEDIWISTFHSMCVRILRRDIDRIRYDRSFSILDASD
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCH
QLTAIKQVLKELNLDPKKYEPRTLLGMISNHKNELRTAKDAAALVGNNPYERIISDVYTA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
YEKKLKQNNVLDFDDLIMKTIHLFQEAPDVLAFYQKKFKYIHVDEYQDTNRAQYTLVKLL
HHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
AQAHENLCVVGDSDQSIYRWRGADIANILTFEKDYPSAHVILLEQNYRSTKRILEAANGV
HHHCCCEEEECCCCCCEEEECCCCHHHHHEECCCCCCEEEEEEECCCHHHHHHHHHHCHH
IQNNASRKEKNLWTENDEGEKLLLHVASDDRDEAFFIMNQMKELKMEGMDYGQMAVLYRT
HHCCCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NAQSRGLEEMLLKSNIPYKMVGGTKFYERKEIKDVLAYLRLIANPDEDISFVRVVNEPKR
CCCCCCHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
GIGAATIDKLGDFADMQGVSLMDAIRDIELSGIAPRTATKLADFRQMMMDLRQMADYLSI
CCCHHHHHHHCCHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SELVEEVLKKTGYEEMLKIEKTLEAESRLENLQEFLSVAQNFEKESDEQTLVAFLTDLTL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSDLDSLDEVDESHQVTLMTLHSAKGLEFPVVFLIGMEEGLFPHSRALNDEEEMEEERRL
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHHHCE
AYVGITRAEKRLYLTRAQSRMLYGRFQNNPESRFLHELPETLLERSGKARKPMPWNPVES
EEEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGKLPVNGFSSKPKPKLAQSSGAETVGWNVGDKANHKKWGQGTVVQTRGEGDQLEVDIAF
CCCCCCCCCCCCCCCCHHHCCCCCEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEC
PEVGIKRLLAKFAPIEKA
CHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]