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The map label for this gene is purF [H]

Identifier: 229917951

GI number: 229917951

Start: 2177567

End: 2178976

Strand: Direct

Name: purF [H]

Synonym: EAT1b_2230

Alternate gene names: 229917951

Gene position: 2177567-2178976 (Clockwise)

Preceding gene: 229917950

Following gene: 229917952

Centisome position: 72.59

GC content: 53.62

Gene sequence:

>1410_bases
ATGTTATATGACATCCGAGGCATGAACGAGGAGTGCGGCGTCTTCGGTGTGTTCGGTCACATCGAAGCGTCACACCTAGC
CTATTACGGACTGCACAGCTTGCAACACCGTGGCCAAGAAGGAACGGGAATCGTCACGGCAGACGGAGAGCGTCTCCACG
CGAAACGTGGACTCGGACTCGTCACCGAAGTGTTTGACGAAACATCTCTCGACGCACTCACCGGGAACCATGCGATTGGA
CACGTCCGCTACTCGACGGCTGGCGGCAACACGCTCGAGAATGTTCAACCGCTCCATTTCAAGTCGTTGACTGGCGATTT
GGCGATGGCGCATAACGGAAACCTCGTGAATGCGAACGAGTTGAAACATATGCTCGAAGCGGAAGGTGCGATTTTCCAGA
CGACGTCCGACACGGAAGTCGTGGCCCATCTCATCAAGCGGAGTCGCGGCAATTCGTTGAAAGAACGGATCGCTGACAGC
TTGGCCCGTCTCGTCGGGGCATTCGCATTCCTATTCTTGACGGAAGATGCACTGTACGTCGGTGTTGATGTGCATGGGTT
ACGTCCATTGTCACTTGGACAAACCGAAGACGGGGCTTACGTGTTCGCTTCGGAAACGTGTGCGTTTGACGTCGTGGGGG
CAACGTACGTACGAGATGTTGAACCAGGAGAATTGCTCATGGTCACGAATGAAGGGATCACGTCGGAGCGATTCAGTGAA
GCGGCAGTCCGTGCCATGTGTTCAATGGAATACATCTACTTCTCACGACCGGACTCAATCATCGATGGCATCAACGTGCA
TACGGCACGTAAAGCCATGGGGAAAAAGATGTTTGAAGAAGCACCGGTCGAAGCGGATGTCGTGACAGGTGTGCCGGATT
CAAGTATTTCCGCGGCAATCGGCTACGCTGAGGCGAGCGGAATCCCATACGAGATGGGTCTCATTAAAAACCGATACGTC
GGACGGACGTTCATTCAACCGTCCCAGGAACTACGGGAGCGCGGCGTGAAGATGAAGTTGTCAGCGTTACGCGGTGTCGT
GAACGGGAAACGCGTCATCATGGTCGATGATTCCATCGTCCGTGGAACGACGAGTCGCCGAATCGTCACGTTACTTCGAG
AGGCAGGCGCGACGGAAGTGCATGTGCGCATCACGTCACCACCAATCAAACATCCGTGTTACTACGGAATCGATACATCC
ACCAAGGAAGAGTTAATTGCGGCAACCAAATCCATTGAAGAAATCAGAGAAGAGATTGGTGCCGATTCACTAGCGTTCCT
CACATTGGATGGAACGGTCGAAGCCATCGATCGTCCATTCGAAGGAGCACTACGCGGGCAGTGTGCTGCATGCTTCACGG
GACTTTATCCGACTGAACTTGCAGAACTCGTGACAGAGACAAAGGCTTAA

Upstream 100 bases:

>100_bases
GGACAGTTACAGAAGCATCCACACTTGTCGTTCGTGCAAGCGACCGCTTGATTGAAATGGACGTACAAGAGTTGGAACGT
GTATGGGAAGGAGCTATTCC

Downstream 100 bases:

>100_bases
GGGGGAAAAGCGATGAGCGTTCGTTATGAACAAGCGGGTGTGAATTTAGAAGCAGGGTACGAGGCCGTATCCCGTATGAA
ACGTCATGTCGCTCGGACAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MLYDIRGMNEECGVFGVFGHIEASHLAYYGLHSLQHRGQEGTGIVTADGERLHAKRGLGLVTEVFDETSLDALTGNHAIG
HVRYSTAGGNTLENVQPLHFKSLTGDLAMAHNGNLVNANELKHMLEAEGAIFQTTSDTEVVAHLIKRSRGNSLKERIADS
LARLVGAFAFLFLTEDALYVGVDVHGLRPLSLGQTEDGAYVFASETCAFDVVGATYVRDVEPGELLMVTNEGITSERFSE
AAVRAMCSMEYIYFSRPDSIIDGINVHTARKAMGKKMFEEAPVEADVVTGVPDSSISAAIGYAEASGIPYEMGLIKNRYV
GRTFIQPSQELRERGVKMKLSALRGVVNGKRVIMVDDSIVRGTTSRRIVTLLREAGATEVHVRITSPPIKHPCYYGIDTS
TKEELIAATKSIEEIREEIGADSLAFLTLDGTVEAIDRPFEGALRGQCAACFTGLYPTELAELVTETKA

Sequences:

>Translated_469_residues
MLYDIRGMNEECGVFGVFGHIEASHLAYYGLHSLQHRGQEGTGIVTADGERLHAKRGLGLVTEVFDETSLDALTGNHAIG
HVRYSTAGGNTLENVQPLHFKSLTGDLAMAHNGNLVNANELKHMLEAEGAIFQTTSDTEVVAHLIKRSRGNSLKERIADS
LARLVGAFAFLFLTEDALYVGVDVHGLRPLSLGQTEDGAYVFASETCAFDVVGATYVRDVEPGELLMVTNEGITSERFSE
AAVRAMCSMEYIYFSRPDSIIDGINVHTARKAMGKKMFEEAPVEADVVTGVPDSSISAAIGYAEASGIPYEMGLIKNRYV
GRTFIQPSQELRERGVKMKLSALRGVVNGKRVIMVDDSIVRGTTSRRIVTLLREAGATEVHVRITSPPIKHPCYYGIDTS
TKEELIAATKSIEEIREEIGADSLAFLTLDGTVEAIDRPFEGALRGQCAACFTGLYPTELAELVTETKA
>Mature_469_residues
MLYDIRGMNEECGVFGVFGHIEASHLAYYGLHSLQHRGQEGTGIVTADGERLHAKRGLGLVTEVFDETSLDALTGNHAIG
HVRYSTAGGNTLENVQPLHFKSLTGDLAMAHNGNLVNANELKHMLEAEGAIFQTTSDTEVVAHLIKRSRGNSLKERIADS
LARLVGAFAFLFLTEDALYVGVDVHGLRPLSLGQTEDGAYVFASETCAFDVVGATYVRDVEPGELLMVTNEGITSERFSE
AAVRAMCSMEYIYFSRPDSIIDGINVHTARKAMGKKMFEEAPVEADVVTGVPDSSISAAIGYAEASGIPYEMGLIKNRYV
GRTFIQPSQELRERGVKMKLSALRGVVNGKRVIMVDDSIVRGTTSRRIVTLLREAGATEVHVRITSPPIKHPCYYGIDTS
TKEELIAATKSIEEIREEIGADSLAFLTLDGTVEAIDRPFEGALRGQCAACFTGLYPTELAELVTETKA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=511, Percent_Identity=39.1389432485323, Blast_Score=360, Evalue=2e-99,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=38.5106382978723, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1790167, Length=165, Percent_Identity=27.8787878787879, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=41.8410041841004, Blast_Score=347, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6323958, Length=452, Percent_Identity=35.8407079646018, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI28573187, Length=499, Percent_Identity=40.4809619238477, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24659598, Length=495, Percent_Identity=40.2020202020202, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659604, Length=463, Percent_Identity=41.2526997840173, Blast_Score=350, Evalue=1e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50925; Mature: 50925

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYDIRGMNEECGVFGVFGHIEASHLAYYGLHSLQHRGQEGTGIVTADGERLHAKRGLGL
CEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCH
VTEVFDETSLDALTGNHAIGHVRYSTAGGNTLENVQPLHFKSLTGDLAMAHNGNLVNANE
HHHHHCCCCCHHHCCCCEEEEEEEECCCCCCHHCCCCCEEHHCCCCEEEECCCCEECHHH
LKHMLEAEGAIFQTTSDTEVVAHLIKRSRGNSLKERIADSLARLVGAFAFLFLTEDALYV
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GVDVHGLRPLSLGQTEDGAYVFASETCAFDVVGATYVRDVEPGELLMVTNEGITSERFSE
EEEECCCCCCCCCCCCCCCEEEEECCCEEEECCHHHEECCCCCCEEEEECCCCCHHHHHH
AAVRAMCSMEYIYFSRPDSIIDGINVHTARKAMGKKMFEEAPVEADVVTGVPDSSISAAI
HHHHHHHHHHEEEECCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHC
GYAEASGIPYEMGLIKNRYVGRTFIQPSQELRERGVKMKLSALRGVVNGKRVIMVDDSIV
CHHHCCCCCEEHHHHHCCCCCCHHCCCHHHHHHCCCEEEHHHHHHHCCCCEEEEECCHHH
RGTTSRRIVTLLREAGATEVHVRITSPPIKHPCYYGIDTSTKEELIAATKSIEEIREEIG
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
ADSLAFLTLDGTVEAIDRPFEGALRGQCAACFTGLYPTELAELVTETKA
CCCEEEEEECCCHHHHCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLYDIRGMNEECGVFGVFGHIEASHLAYYGLHSLQHRGQEGTGIVTADGERLHAKRGLGL
CEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHCCCCH
VTEVFDETSLDALTGNHAIGHVRYSTAGGNTLENVQPLHFKSLTGDLAMAHNGNLVNANE
HHHHHCCCCCHHHCCCCEEEEEEEECCCCCCHHCCCCCEEHHCCCCEEEECCCCEECHHH
LKHMLEAEGAIFQTTSDTEVVAHLIKRSRGNSLKERIADSLARLVGAFAFLFLTEDALYV
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
GVDVHGLRPLSLGQTEDGAYVFASETCAFDVVGATYVRDVEPGELLMVTNEGITSERFSE
EEEECCCCCCCCCCCCCCCEEEEECCCEEEECCHHHEECCCCCCEEEEECCCCCHHHHHH
AAVRAMCSMEYIYFSRPDSIIDGINVHTARKAMGKKMFEEAPVEADVVTGVPDSSISAAI
HHHHHHHHHHEEEECCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHC
GYAEASGIPYEMGLIKNRYVGRTFIQPSQELRERGVKMKLSALRGVVNGKRVIMVDDSIV
CHHHCCCCCEEHHHHHCCCCCCHHCCCHHHHHHCCCEEEHHHHHHHCCCCEEEEECCHHH
RGTTSRRIVTLLREAGATEVHVRITSPPIKHPCYYGIDTSTKEELIAATKSIEEIREEIG
CCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHC
ADSLAFLTLDGTVEAIDRPFEGALRGQCAACFTGLYPTELAELVTETKA
CCCEEEEEECCCHHHHCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]