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The map label for this gene is carA [H]

Identifier: 229917790

GI number: 229917790

Start: 2018272

End: 2019324

Strand: Direct

Name: carA [H]

Synonym: EAT1b_2068

Alternate gene names: 229917790

Gene position: 2018272-2019324 (Clockwise)

Preceding gene: 229917789

Following gene: 229917791

Centisome position: 67.28

GC content: 51.85

Gene sequence:

>1053_bases
ATGAACGGAAGCATCAGCTTAATGAACGGAAAGCATTTTACAGGAACATGGGAGACGAGTGTGCGACACGCAAGTGGCGA
AGTCGTATTCTTCACCGGGATGACGGGCTATCAAGAAGTGTTGACCGACCCGTCATATGCTGGACAAATCATCGCATTCA
CTTATCCGCTGATCGGACAATATGGACTGGAATCAGACGGGAGCGAAAGTGCTCGCATTCAAGTGGCCGGCGTCCTCGTG
CAATCACTTGCCGATTCTGGGGATCAGTCTGACTTAAAGGATTGGTTAGAGCACGAAGGGATTCCAGTTCTGTCACAAGT
CGATGTGCGTGCACTCGTTCATGTGTTGCGTGATGAAGGCGATCAGTTTGGGATGATGGCAGAGGAGGCACATGAAACGT
TGGACGTACCCGATATCACTCGGCTGACAGAGCGCGTCACAACAGCGTCTATCGTTCAGGAAGGGAACGGGGAGAAAGGG
CATGTCGTCTGTCTCGACTTCGGGGTGAAGGCGTCGATGATCGGGGAAATGGTGAAACGTGGATATCGTGTGACGAGTGT
TCCGTACCATACGTCAAAAGAGGTCGTCGAAGGGTTGCGACCGGATGGGATTCTAGTAACGAACGGACCCGGGGACCCGC
GAGACCACCTCGGGCAAATAGAAGTCATCCGGGAATTGGCGTTATCGTATCCGACCCTTGGGATCTGCATGGGGCATCAA
TTGCTCGCACTCGCATTCGGTTGCAAGATTGAAAAGCAAACATACGGACATCGTGGTTCGAATCATCCGGTCCGAAACGT
CAAGACGAACGACATATGGATGACGTCGCAAAACCACGGGTATGTGGTCGAGCGTATGAGTATCGAACAGAGCCCGCTTG
AATTGCTGTATGAGAACGTGAACGATCACTCGGTGGAAGGGTTGATTCATCCGAATGCATCCATTTTCACGACACAGTTC
CATCCGGAGGCGAGTCCTGGACCAACGGACGCGCAGTCCGTCTTCGACCGTTTTGATGAAATGATTCAAGAAGGAGTGAA
CGTGTATGCATAA

Upstream 100 bases:

>100_bases
CGCACGTCATCCGATTCCTGCCTTCCCTATTGGTCACAAAATCGGAGATCGACGAGGCATTGACGAAACTTGAACAAATC
TTACAGAAGGAGGGCGTCGT

Downstream 100 bases:

>100_bases
AAAAGTACTCGTCATTGGTTCAGGACCAATCGTGATTGGGCAGGCCGCAGAGTTTGATTATTCCGGAACGCAGGCGTGCC
AGGCGCTTCGTGAAGCCGGG

Product: carbamoyl-phosphate synthase, small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNGSISLMNGKHFTGTWETSVRHASGEVVFFTGMTGYQEVLTDPSYAGQIIAFTYPLIGQYGLESDGSESARIQVAGVLV
QSLADSGDQSDLKDWLEHEGIPVLSQVDVRALVHVLRDEGDQFGMMAEEAHETLDVPDITRLTERVTTASIVQEGNGEKG
HVVCLDFGVKASMIGEMVKRGYRVTSVPYHTSKEVVEGLRPDGILVTNGPGDPRDHLGQIEVIRELALSYPTLGICMGHQ
LLALAFGCKIEKQTYGHRGSNHPVRNVKTNDIWMTSQNHGYVVERMSIEQSPLELLYENVNDHSVEGLIHPNASIFTTQF
HPEASPGPTDAQSVFDRFDEMIQEGVNVYA

Sequences:

>Translated_350_residues
MNGSISLMNGKHFTGTWETSVRHASGEVVFFTGMTGYQEVLTDPSYAGQIIAFTYPLIGQYGLESDGSESARIQVAGVLV
QSLADSGDQSDLKDWLEHEGIPVLSQVDVRALVHVLRDEGDQFGMMAEEAHETLDVPDITRLTERVTTASIVQEGNGEKG
HVVCLDFGVKASMIGEMVKRGYRVTSVPYHTSKEVVEGLRPDGILVTNGPGDPRDHLGQIEVIRELALSYPTLGICMGHQ
LLALAFGCKIEKQTYGHRGSNHPVRNVKTNDIWMTSQNHGYVVERMSIEQSPLELLYENVNDHSVEGLIHPNASIFTTQF
HPEASPGPTDAQSVFDRFDEMIQEGVNVYA
>Mature_350_residues
MNGSISLMNGKHFTGTWETSVRHASGEVVFFTGMTGYQEVLTDPSYAGQIIAFTYPLIGQYGLESDGSESARIQVAGVLV
QSLADSGDQSDLKDWLEHEGIPVLSQVDVRALVHVLRDEGDQFGMMAEEAHETLDVPDITRLTERVTTASIVQEGNGEKG
HVVCLDFGVKASMIGEMVKRGYRVTSVPYHTSKEVVEGLRPDGILVTNGPGDPRDHLGQIEVIRELALSYPTLGICMGHQ
LLALAFGCKIEKQTYGHRGSNHPVRNVKTNDIWMTSQNHGYVVERMSIEQSPLELLYENVNDHSVEGLIHPNASIFTTQF
HPEASPGPTDAQSVFDRFDEMIQEGVNVYA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=363, Percent_Identity=36.6391184573003, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI21361331, Length=339, Percent_Identity=35.1032448377581, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI169790915, Length=339, Percent_Identity=35.1032448377581, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1786215, Length=371, Percent_Identity=36.6576819407008, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=34.8773841961853, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324878, Length=366, Percent_Identity=37.1584699453552, Blast_Score=225, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=34.010152284264, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45555749, Length=347, Percent_Identity=34.5821325648415, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24642586, Length=347, Percent_Identity=34.8703170028818, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 38472; Mature: 38472

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSISLMNGKHFTGTWETSVRHASGEVVFFTGMTGYQEVLTDPSYAGQIIAFTYPLIGQ
CCCCEEEECCCEECCCHHHHHEECCCCEEEEECCCCHHHHHCCCCCCCEEEEEECHHHHC
YGLESDGSESARIQVAGVLVQSLADSGDQSDLKDWLEHEGIPVLSQVDVRALVHVLRDEG
CCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DQFGMMAEEAHETLDVPDITRLTERVTTASIVQEGNGEKGHVVCLDFGVKASMIGEMVKR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHC
GYRVTSVPYHTSKEVVEGLRPDGILVTNGPGDPRDHLGQIEVIRELALSYPTLGICMGHQ
CEEEEECCCCCHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLALAFGCKIEKQTYGHRGSNHPVRNVKTNDIWMTSQNHGYVVERMSIEQSPLELLYENV
HHHHHHCCEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCC
NDHSVEGLIHPNASIFTTQFHPEASPGPTDAQSVFDRFDEMIQEGVNVYA
CCCCCCEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNGSISLMNGKHFTGTWETSVRHASGEVVFFTGMTGYQEVLTDPSYAGQIIAFTYPLIGQ
CCCCEEEECCCEECCCHHHHHEECCCCEEEEECCCCHHHHHCCCCCCCEEEEEECHHHHC
YGLESDGSESARIQVAGVLVQSLADSGDQSDLKDWLEHEGIPVLSQVDVRALVHVLRDEG
CCCCCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
DQFGMMAEEAHETLDVPDITRLTERVTTASIVQEGNGEKGHVVCLDFGVKASMIGEMVKR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHC
GYRVTSVPYHTSKEVVEGLRPDGILVTNGPGDPRDHLGQIEVIRELALSYPTLGICMGHQ
CEEEEECCCCCHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLALAFGCKIEKQTYGHRGSNHPVRNVKTNDIWMTSQNHGYVVERMSIEQSPLELLYENV
HHHHHHCCEEEHHHCCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHCC
NDHSVEGLIHPNASIFTTQFHPEASPGPTDAQSVFDRFDEMIQEGVNVYA
CCCCCCEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]