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The map label for this gene is merA [H]

Identifier: 229917738

GI number: 229917738

Start: 1967490

End: 1968914

Strand: Direct

Name: merA [H]

Synonym: EAT1b_2015

Alternate gene names: 229917738

Gene position: 1967490-1968914 (Clockwise)

Preceding gene: 229917737

Following gene: 229917742

Centisome position: 65.59

GC content: 51.37

Gene sequence:

>1425_bases
ATGAAGGACTATCAGTTGATTGTCATCGGTGGTGGTGCTGCCGGGATGACAGTGGCCGCAGGGGCTGCGAATTTAGGCGC
GAAAGTCGCGCTTGTTGAAAAGAACAAGCATTTAGGTGGTGACTGTCTGCATGTCGGATGTGTGCCATCGAAAGCGTTGA
TTGCTGCTTCAAAAGAAGCGAATGCGATGATGCGCGCGGCCCGAGTTCTCGGAACCGAGTTCAATGGACAAGAGCATTAT
CAAATGTCAAAAGCGCGTGTCGACAAAGCACGTGCCATTATTCAAGACCATGACGGAACAGAACGTTTTGAAAAGATTGG
CGTCGACGTCTATATCGGAGAAGCCTCGTTCCAAAGTCCGCACGAATTAAAAGTAGGGAACGAGTTATTGATCGGGAAAA
AATTTGTCATCTCGACGGGGTCGAAACCGACAGTTCCTCCAATTGATGGACTCGATCAAGTCCCATACTTAACGAACGAA
ACGGTCTTTGAAGAAGAGACGCTCCCGGAGAGCCTGATTGTCATCGGCGCCGGAACGGTCGGGCTTGAATTGGCCCAGTC
CTTTGTCCGACTCGGCTCACAAGTGACCGTGCTCGAGGCGCAGGATGATTTCCTACCTAAAGAAGACGAGGACGTCGCCA
CCTATTTGAAATCTCAACTCGAGATGGCGATGCGCCTAAAAATCGGCGTAAAGGTCAATCGAGTCGATGAGGTCGATGGC
CGCATTCGTGTCACGACAGAGGTGAACGGCGAAACGGTCGAATATGAAGCGGACAAACTGCTGATGGCGACAGGTCGCGT
TCCCCGAATCGATGCGCTCCGTCTCGACCGGGCGAACGTTCAGGTGACGAAAGGGTATATCGATGTCTCGTCGTCATATC
GGACGTCACAATCTCATATCTTTGCCATTGGCGATACGATCAAAACGCTCCCATTCACACATGCCGCTGGAGAAGAAGGG
AAGGCGGTTGTCGCGAACGCGCTCTTTGGATTATGGAACAAGGTCGACTACAGCAAGCTCCCGTGGATCACATTCACAGA
TCCTGAGGTGTTCCATGTCGGGATGACAGAGAAAGAAGCGCGCGAGCGTTACGGCGACGACATTGAGACGTATGAAGCGA
AACTGTCCGAAGTGGACCGCTTCATTGCTGAACGCCAAGAAGCAGGATTTGTCAAAGTGATCACGAAGAAAAACGGAAAA
ATCATCGGAGCACATGCAGTCGGTGAATCGGCGGGGGATTGGATGCAAATTGTCGTCTATGCGATGCAACGTGGCGACAA
AATCGGGAAGCTGTCCCGTATGATTTACCCGTATCCGTCGCGAAGTGAGGCCGTGAAGAAAGTGACAGACCTTTACTGGC
GGAAACGTTTGTTCGAAAGTCCGCTCACCGAACTTGCGAGACGCTACTTTAAATTGGTGCAATAA

Upstream 100 bases:

>100_bases
AAGTCGAGTTTCGATCTGCGTCGCGTGTCGGCTATTCAGACTTTGGCGTGAATCGAAAACGAATGGAAGATGTGAGCGAA
CGATACCGGAGGTTGATTCG

Downstream 100 bases:

>100_bases
CACAAATCACCCACTCCAACGAGTGGGTGATTATTAGTTTGGTAAGGTGATGGTTTCAATCATCTTCGGTTCGCCTTCAG
AAAGGTCGACGAGTGGTTTG

Product: pyridine nucleotide-disulphide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKDYQLIVIGGGAAGMTVAAGAANLGAKVALVEKNKHLGGDCLHVGCVPSKALIAASKEANAMMRAARVLGTEFNGQEHY
QMSKARVDKARAIIQDHDGTERFEKIGVDVYIGEASFQSPHELKVGNELLIGKKFVISTGSKPTVPPIDGLDQVPYLTNE
TVFEEETLPESLIVIGAGTVGLELAQSFVRLGSQVTVLEAQDDFLPKEDEDVATYLKSQLEMAMRLKIGVKVNRVDEVDG
RIRVTTEVNGETVEYEADKLLMATGRVPRIDALRLDRANVQVTKGYIDVSSSYRTSQSHIFAIGDTIKTLPFTHAAGEEG
KAVVANALFGLWNKVDYSKLPWITFTDPEVFHVGMTEKEARERYGDDIETYEAKLSEVDRFIAERQEAGFVKVITKKNGK
IIGAHAVGESAGDWMQIVVYAMQRGDKIGKLSRMIYPYPSRSEAVKKVTDLYWRKRLFESPLTELARRYFKLVQ

Sequences:

>Translated_474_residues
MKDYQLIVIGGGAAGMTVAAGAANLGAKVALVEKNKHLGGDCLHVGCVPSKALIAASKEANAMMRAARVLGTEFNGQEHY
QMSKARVDKARAIIQDHDGTERFEKIGVDVYIGEASFQSPHELKVGNELLIGKKFVISTGSKPTVPPIDGLDQVPYLTNE
TVFEEETLPESLIVIGAGTVGLELAQSFVRLGSQVTVLEAQDDFLPKEDEDVATYLKSQLEMAMRLKIGVKVNRVDEVDG
RIRVTTEVNGETVEYEADKLLMATGRVPRIDALRLDRANVQVTKGYIDVSSSYRTSQSHIFAIGDTIKTLPFTHAAGEEG
KAVVANALFGLWNKVDYSKLPWITFTDPEVFHVGMTEKEARERYGDDIETYEAKLSEVDRFIAERQEAGFVKVITKKNGK
IIGAHAVGESAGDWMQIVVYAMQRGDKIGKLSRMIYPYPSRSEAVKKVTDLYWRKRLFESPLTELARRYFKLVQ
>Mature_474_residues
MKDYQLIVIGGGAAGMTVAAGAANLGAKVALVEKNKHLGGDCLHVGCVPSKALIAASKEANAMMRAARVLGTEFNGQEHY
QMSKARVDKARAIIQDHDGTERFEKIGVDVYIGEASFQSPHELKVGNELLIGKKFVISTGSKPTVPPIDGLDQVPYLTNE
TVFEEETLPESLIVIGAGTVGLELAQSFVRLGSQVTVLEAQDDFLPKEDEDVATYLKSQLEMAMRLKIGVKVNRVDEVDG
RIRVTTEVNGETVEYEADKLLMATGRVPRIDALRLDRANVQVTKGYIDVSSSYRTSQSHIFAIGDTIKTLPFTHAAGEEG
KAVVANALFGLWNKVDYSKLPWITFTDPEVFHVGMTEKEARERYGDDIETYEAKLSEVDRFIAERQEAGFVKVITKKNGK
IIGAHAVGESAGDWMQIVVYAMQRGDKIGKLSRMIYPYPSRSEAVKKVTDLYWRKRLFESPLTELARRYFKLVQ

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=28.1720430107527, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=28.1449893390192, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.8481012658228, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI33519430, Length=482, Percent_Identity=26.9709543568465, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519428, Length=482, Percent_Identity=26.9709543568465, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI33519426, Length=482, Percent_Identity=26.9709543568465, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI148277065, Length=482, Percent_Identity=26.9709543568465, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI148277071, Length=482, Percent_Identity=26.9709543568465, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI291045266, Length=473, Percent_Identity=28.7526427061311, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=26.3829787234043, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786307, Length=480, Percent_Identity=31.25, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=29.004329004329, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87082354, Length=477, Percent_Identity=25.9958071278826, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=28.7015945330296, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789065, Length=210, Percent_Identity=28.0952380952381, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=28.9130434782609, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=501, Percent_Identity=27.9441117764471, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71982272, Length=494, Percent_Identity=27.5303643724696, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71983429, Length=432, Percent_Identity=26.1574074074074, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=432, Percent_Identity=26.1574074074074, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=30.5439330543933, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=29.0526315789474, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=25.8333333333333, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI21358499, Length=454, Percent_Identity=29.7356828193833, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24640549, Length=470, Percent_Identity=28.5106382978723, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640553, Length=470, Percent_Identity=28.5106382978723, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28.2105263157895, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=27.7894736842105, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 52368; Mature: 52368

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYQLIVIGGGAAGMTVAAGAANLGAKVALVEKNKHLGGDCLHVGCVPSKALIAASKEA
CCCEEEEEEECCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHEEECHHH
NAMMRAARVLGTEFNGQEHYQMSKARVDKARAIIQDHDGTERFEKIGVDVYIGEASFQSP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCC
HELKVGNELLIGKKFVISTGSKPTVPPIDGLDQVPYLTNETVFEEETLPESLIVIGAGTV
CCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCHH
GLELAQSFVRLGSQVTVLEAQDDFLPKEDEDVATYLKSQLEMAMRLKIGVKVNRVDEVDG
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEECEEEECEECCCC
RIRVTTEVNGETVEYEADKLLMATGRVPRIDALRLDRANVQVTKGYIDVSSSYRTSQSHI
EEEEEEECCCCEEEECCCEEEEECCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCEE
FAIGDTIKTLPFTHAAGEEGKAVVANALFGLWNKVDYSKLPWITFTDPEVFHVGMTEKEA
EEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHH
RERYGDDIETYEAKLSEVDRFIAERQEAGFVKVITKKNGKIIGAHAVGESAGDWMQIVVY
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHH
AMQRGDKIGKLSRMIYPYPSRSEAVKKVTDLYWRKRLFESPLTELARRYFKLVQ
HHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDYQLIVIGGGAAGMTVAAGAANLGAKVALVEKNKHLGGDCLHVGCVPSKALIAASKEA
CCCEEEEEEECCCCCCEEECCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHEEECHHH
NAMMRAARVLGTEFNGQEHYQMSKARVDKARAIIQDHDGTERFEKIGVDVYIGEASFQSP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEECCCCCCCC
HELKVGNELLIGKKFVISTGSKPTVPPIDGLDQVPYLTNETVFEEETLPESLIVIGAGTV
CCCCCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEECCHH
GLELAQSFVRLGSQVTVLEAQDDFLPKEDEDVATYLKSQLEMAMRLKIGVKVNRVDEVDG
HHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEECEEEECEECCCC
RIRVTTEVNGETVEYEADKLLMATGRVPRIDALRLDRANVQVTKGYIDVSSSYRTSQSHI
EEEEEEECCCCEEEECCCEEEEECCCCCCCCEEEECCCCEEEEEEEEEECCCCCCCCCEE
FAIGDTIKTLPFTHAAGEEGKAVVANALFGLWNKVDYSKLPWITFTDPEVFHVGMTEKEA
EEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHH
RERYGDDIETYEAKLSEVDRFIAERQEAGFVKVITKKNGKIIGAHAVGESAGDWMQIVVY
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEEECCCCCCHHHHHHHH
AMQRGDKIGKLSRMIYPYPSRSEAVKKVTDLYWRKRLFESPLTELARRYFKLVQ
HHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]