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The map label for this gene is yclK [H]

Identifier: 229917736

GI number: 229917736

Start: 1965684

End: 1967063

Strand: Direct

Name: yclK [H]

Synonym: EAT1b_2013

Alternate gene names: 229917736

Gene position: 1965684-1967063 (Clockwise)

Preceding gene: 229917735

Following gene: 229917737

Centisome position: 65.53

GC content: 49.49

Gene sequence:

>1380_bases
ATGAAACAACAGTTAGTCATTCGCATGATGATTGTCGTGACGATCGTCTTGCTCTTACTCGTCAGCGTTTTGTCTTACGG
GACGTATCGCTACTATTACGAGTATGAGTCAGAAGCGTTACTGAGTCATGCGACCACGAGTGCATATCTATACCCTCAAG
CCCAAGTAGGGTTTGAGACGCCGAGTGCCTTTTCTGTCCTCGTCCGGACATTCGGATATCCTGGAACTGATTTGACGATT
GTCGACCGGAGCGGTTCGATTGTCAAAACGACGGGACAACGAACAAGGCAGGCCTCATTCTCTCAAGAACAGTTACAGCA
ATTGTCGCAAGGAGAGTCCGTCGTGCAGGACTATGACGTCGGGGATGAACATTACTTGTCAGTCACGAGTCCCATTATGA
CGAACGATACGTCCGAGTTTGCCATCAGTTTCACGCGACCGCTTGAAGATTTAGACACGCTCATTCGCGAAATTTGGATG
ACGGTCGTCATGATTGGCGTCTTGATCTGGTTGCTCGCCCTATACACCGTCATCCGGATTGCCGATCGCTTCGTCAGACC
GATTCGTCACATCATCGAGGCGGCAGATGAGATGGCTCAGGGCGACTTCGATGTATCCGTTGAGTCAAATGAGAAACATG
AGCTTGGAGCCTTGGCTCGAACGCTCACGTATTTAGGCGCGAAAGTCCAGCAGCATCAGTCGACACGTAACGAATTCCTC
TCGAGCGTGTCGCACGAACTCAGGACACCACTCACATCGATTAAGGGATGGACCGAGACGATTCGGGGTGGTGATGAGAA
ACTGTCTGAAGAGACAGAGCTCGGTTTAAGCATCATCCATTCGGAGACAGAGCGCATGATCGGTCTCGTCGAACAGCTTC
TTGACTATACAAAAATTGAAGAGAGTCGGTTGGTCTTGTATCGCCAAGAACTGGACCTGGTCTCTTTGTTACGTCAAGTG
ACACTGCAATTAAAACAGTCACTTGAAGACAAAGGCATCACACTCATCGAACGCTATCCTCAAAAATCGGTTGGACTATT
TGATGAGAATCGATTGAAACAAGTGTTCTTAAACATTCTCGAGAACGCGATTCGTTTCTCACCAGACGGAGGAACGTTGA
CCATCCGCTTCGTGCAATCGGAACGTGTCGCCTTCATCTCCATTATCGATGAAGGTCCCGGCATTTCACGAGAGCATTTG
CAGCGCATCACCGAGATGTTCTATCAGGCTCATGTCACAGAAGGTAGTAGCGGGCTTGGGCTTGCGATTTCAAAAGAATT
GATCGAAGCGCATGAAGGCAAACTCGTCATCGAGAGCGAGGTCGGACACGGGACGACAGTGACCGTCTTATTGCCGCTCG
ACCCGAGTGAACTACATTAA

Upstream 100 bases:

>100_bases
ACATTCGTCGCTTGCGACAAAAAATCGAAGATGACCCGAGTCATCCGGTGTACATCGAGACGTTGTGGGGTCACGGATAT
AAATGGCGGAGACATGAGGC

Downstream 100 bases:

>100_bases
TAGGATTAAACCTCCCGAATTCAGGCTACATATAGATGTATGTAGACTGAGGGAGGTTTTTATAATGGCCAATCAATCTT
TGAAGCCGTTAAAAGGAAGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKQQLVIRMMIVVTIVLLLLVSVLSYGTYRYYYEYESEALLSHATTSAYLYPQAQVGFETPSAFSVLVRTFGYPGTDLTI
VDRSGSIVKTTGQRTRQASFSQEQLQQLSQGESVVQDYDVGDEHYLSVTSPIMTNDTSEFAISFTRPLEDLDTLIREIWM
TVVMIGVLIWLLALYTVIRIADRFVRPIRHIIEAADEMAQGDFDVSVESNEKHELGALARTLTYLGAKVQQHQSTRNEFL
SSVSHELRTPLTSIKGWTETIRGGDEKLSEETELGLSIIHSETERMIGLVEQLLDYTKIEESRLVLYRQELDLVSLLRQV
TLQLKQSLEDKGITLIERYPQKSVGLFDENRLKQVFLNILENAIRFSPDGGTLTIRFVQSERVAFISIIDEGPGISREHL
QRITEMFYQAHVTEGSSGLGLAISKELIEAHEGKLVIESEVGHGTTVTVLLPLDPSELH

Sequences:

>Translated_459_residues
MKQQLVIRMMIVVTIVLLLLVSVLSYGTYRYYYEYESEALLSHATTSAYLYPQAQVGFETPSAFSVLVRTFGYPGTDLTI
VDRSGSIVKTTGQRTRQASFSQEQLQQLSQGESVVQDYDVGDEHYLSVTSPIMTNDTSEFAISFTRPLEDLDTLIREIWM
TVVMIGVLIWLLALYTVIRIADRFVRPIRHIIEAADEMAQGDFDVSVESNEKHELGALARTLTYLGAKVQQHQSTRNEFL
SSVSHELRTPLTSIKGWTETIRGGDEKLSEETELGLSIIHSETERMIGLVEQLLDYTKIEESRLVLYRQELDLVSLLRQV
TLQLKQSLEDKGITLIERYPQKSVGLFDENRLKQVFLNILENAIRFSPDGGTLTIRFVQSERVAFISIIDEGPGISREHL
QRITEMFYQAHVTEGSSGLGLAISKELIEAHEGKLVIESEVGHGTTVTVLLPLDPSELH
>Mature_459_residues
MKQQLVIRMMIVVTIVLLLLVSVLSYGTYRYYYEYESEALLSHATTSAYLYPQAQVGFETPSAFSVLVRTFGYPGTDLTI
VDRSGSIVKTTGQRTRQASFSQEQLQQLSQGESVVQDYDVGDEHYLSVTSPIMTNDTSEFAISFTRPLEDLDTLIREIWM
TVVMIGVLIWLLALYTVIRIADRFVRPIRHIIEAADEMAQGDFDVSVESNEKHELGALARTLTYLGAKVQQHQSTRNEFL
SSVSHELRTPLTSIKGWTETIRGGDEKLSEETELGLSIIHSETERMIGLVEQLLDYTKIEESRLVLYRQELDLVSLLRQV
TLQLKQSLEDKGITLIERYPQKSVGLFDENRLKQVFLNILENAIRFSPDGGTLTIRFVQSERVAFISIIDEGPGISREHL
QRITEMFYQAHVTEGSSGLGLAISKELIEAHEGKLVIESEVGHGTTVTVLLPLDPSELH

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=272, Percent_Identity=31.25, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1786600, Length=275, Percent_Identity=33.0909090909091, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788713, Length=231, Percent_Identity=31.6017316017316, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789149, Length=356, Percent_Identity=25.561797752809, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994928, Length=255, Percent_Identity=29.4117647058824, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786783, Length=321, Percent_Identity=27.1028037383178, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082128, Length=304, Percent_Identity=27.3026315789474, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788549, Length=225, Percent_Identity=29.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=29.2181069958848, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=27.6363636363636, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790861, Length=338, Percent_Identity=25.7396449704142, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=29.8165137614679, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=290, Percent_Identity=25.5172413793103, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=30.3418803418803, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790551, Length=292, Percent_Identity=23.6301369863014, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790300, Length=244, Percent_Identity=30.7377049180328, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789403, Length=222, Percent_Identity=28.8288288288288, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=265, Percent_Identity=23.7735849056604, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786912, Length=230, Percent_Identity=25.2173913043478, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787374, Length=356, Percent_Identity=22.752808988764, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51796; Mature: 51796

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQLVIRMMIVVTIVLLLLVSVLSYGTYRYYYEYESEALLSHATTSAYLYPQAQVGFET
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHCCCCEEECCCHHCCCCC
PSAFSVLVRTFGYPGTDLTIVDRSGSIVKTTGQRTRQASFSQEQLQQLSQGESVVQDYDV
CHHHHHHHHHCCCCCCCEEEEECCCCEEECCCHHHHHHCCCHHHHHHHHCCHHHHHHCCC
GDEHYLSVTSPIMTNDTSEFAISFTRPLEDLDTLIREIWMTVVMIGVLIWLLALYTVIRI
CCCCEEEECCCCCCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFVRPIRHIIEAADEMAQGDFDVSVESNEKHELGALARTLTYLGAKVQQHQSTRNEFL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVSHELRTPLTSIKGWTETIRGGDEKLSEETELGLSIIHSETERMIGLVEQLLDYTKIE
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESRLVLYRQELDLVSLLRQVTLQLKQSLEDKGITLIERYPQKSVGLFDENRLKQVFLNIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCCCCHHHHHHHHHHHH
ENAIRFSPDGGTLTIRFVQSERVAFISIIDEGPGISREHLQRITEMFYQAHVTEGSSGLG
HHHHEECCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LAISKELIEAHEGKLVIESEVGHGTTVTVLLPLDPSELH
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHCCC
>Mature Secondary Structure
MKQQLVIRMMIVVTIVLLLLVSVLSYGTYRYYYEYESEALLSHATTSAYLYPQAQVGFET
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHCCCCEEECCCHHCCCCC
PSAFSVLVRTFGYPGTDLTIVDRSGSIVKTTGQRTRQASFSQEQLQQLSQGESVVQDYDV
CHHHHHHHHHCCCCCCCEEEEECCCCEEECCCHHHHHHCCCHHHHHHHHCCHHHHHHCCC
GDEHYLSVTSPIMTNDTSEFAISFTRPLEDLDTLIREIWMTVVMIGVLIWLLALYTVIRI
CCCCEEEECCCCCCCCCCHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADRFVRPIRHIIEAADEMAQGDFDVSVESNEKHELGALARTLTYLGAKVQQHQSTRNEFL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVSHELRTPLTSIKGWTETIRGGDEKLSEETELGLSIIHSETERMIGLVEQLLDYTKIE
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ESRLVLYRQELDLVSLLRQVTLQLKQSLEDKGITLIERYPQKSVGLFDENRLKQVFLNIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCCCCHHHHHHHHHHHH
ENAIRFSPDGGTLTIRFVQSERVAFISIIDEGPGISREHLQRITEMFYQAHVTEGSSGLG
HHHHEECCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LAISKELIEAHEGKLVIESEVGHGTTVTVLLPLDPSELH
HHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]