Click here to switch to the map view.

The map label for this gene is trmH [H]

Identifier: 229917708

GI number: 229917708

Start: 1943537

End: 1944301

Strand: Direct

Name: trmH [H]

Synonym: EAT1b_1985

Alternate gene names: 229917708

Gene position: 1943537-1944301 (Clockwise)

Preceding gene: 229917704

Following gene: 229917709

Centisome position: 64.79

GC content: 49.67

Gene sequence:

>765_bases
ATGGGTTGGAGACAGACCACATCTGCTTTGACGAAGGACGAGCAGATGGAATTAAAAGAAGAGTTATTGCAGAAGGGTGT
ATTGAAGGAGGACGATCTTCCTCTATATGAGATGCTCATGCCAGACCGCATTAAACGGATGTATGAAGTGTTGAATACAC
GAACGAACCATATCACGATTTTGACGGAAGGTGTGGACGATCCGCACAATCAATCCGCCGTGTTGCGTTCGGCCGATGCG
TTCGGGGTACAGACGTTGCACGTCGTCGAAGGACGGGCAAAGTTCAAACCGAACTCGATTATCGCCAAGAGTGCCGATCG
CTGGGTGGACGTCGTCGAGCATGGCTCCATTGAAGAGGCGATTGACGCTTTAAAAGAAAGTGGTTACCAAGTATATGCGA
CCGCGTTAAGTGAAGAGGCGATTCCGCTCGAAGAAGTGGATGTGTCGAAGCCGACCGTTCTTCTATTCGGGAATGAACAT
AGTGGTGTCTCGGAAACAGCACTCCATAAGGCGGACGGGAACTTTGTTATTCCGATGCAAGGGTATGTGCAGAGTTTGAA
CATCTCCGTGGCGGCGGCGATTTCCTTGTACGATGTCACGACACGGTCACGACATATCGTGACGGATGGCTATGAACTGC
CGGTCGAAGATCAAGCGCGCATCTTCAAAGACTGGATGGTCAAGAGCTTACCGAAGCGTACGCTTGGACTATATAAACAA
AAAGGTGTCACGGCTTTTGAACGGGAGACAGGAAATTCAGAATGA

Upstream 100 bases:

>100_bases
TTTGTTTTCATTTTTTGAAGTGTAAAGGGTAGATATCAGGCGAACTTGTCAGTCGAATCGAATCTAGCTACAATATGATA
GGTTGAAGGGAGCGAGAAAG

Downstream 100 bases:

>100_bases
ACGGCGAAATGAACAGACGTACGGAATCTTTGTCCAGAAACTGAAAGGGGATGGCACGATGAGTACGACGAGTTCATTTA
TCGTAAAAGATGTGACGAAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MGWRQTTSALTKDEQMELKEELLQKGVLKEDDLPLYEMLMPDRIKRMYEVLNTRTNHITILTEGVDDPHNQSAVLRSADA
FGVQTLHVVEGRAKFKPNSIIAKSADRWVDVVEHGSIEEAIDALKESGYQVYATALSEEAIPLEEVDVSKPTVLLFGNEH
SGVSETALHKADGNFVIPMQGYVQSLNISVAAAISLYDVTTRSRHIVTDGYELPVEDQARIFKDWMVKSLPKRTLGLYKQ
KGVTAFERETGNSE

Sequences:

>Translated_254_residues
MGWRQTTSALTKDEQMELKEELLQKGVLKEDDLPLYEMLMPDRIKRMYEVLNTRTNHITILTEGVDDPHNQSAVLRSADA
FGVQTLHVVEGRAKFKPNSIIAKSADRWVDVVEHGSIEEAIDALKESGYQVYATALSEEAIPLEEVDVSKPTVLLFGNEH
SGVSETALHKADGNFVIPMQGYVQSLNISVAAAISLYDVTTRSRHIVTDGYELPVEDQARIFKDWMVKSLPKRTLGLYKQ
KGVTAFERETGNSE
>Mature_253_residues
GWRQTTSALTKDEQMELKEELLQKGVLKEDDLPLYEMLMPDRIKRMYEVLNTRTNHITILTEGVDDPHNQSAVLRSADAF
GVQTLHVVEGRAKFKPNSIIAKSADRWVDVVEHGSIEEAIDALKESGYQVYATALSEEAIPLEEVDVSKPTVLLFGNEHS
GVSETALHKADGNFVIPMQGYVQSLNISVAAAISLYDVTTRSRHIVTDGYELPVEDQARIFKDWMVKSLPKRTLGLYKQK
GVTAFERETGNSE

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=212, Percent_Identity=26.4150943396226, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790083, Length=185, Percent_Identity=37.2972972972973, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 28450; Mature: 28318

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWRQTTSALTKDEQMELKEELLQKGVLKEDDLPLYEMLMPDRIKRMYEVLNTRTNHITI
CCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCEEEE
LTEGVDDPHNQSAVLRSADAFGVQTLHVVEGRAKFKPNSIIAKSADRWVDVVEHGSIEEA
EECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
IDALKESGYQVYATALSEEAIPLEEVDVSKPTVLLFGNEHSGVSETALHKADGNFVIPMQ
HHHHHHCCCEEEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHEECCCCEEEEHH
GYVQSLNISVAAAISLYDVTTRSRHIVTDGYELPVEDQARIFKDWMVKSLPKRTLGLYKQ
HHHHHHCEEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
KGVTAFERETGNSE
CCCCHHHCCCCCCC
>Mature Secondary Structure 
GWRQTTSALTKDEQMELKEELLQKGVLKEDDLPLYEMLMPDRIKRMYEVLNTRTNHITI
CCCHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHCCCCCEEEE
LTEGVDDPHNQSAVLRSADAFGVQTLHVVEGRAKFKPNSIIAKSADRWVDVVEHGSIEEA
EECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHH
IDALKESGYQVYATALSEEAIPLEEVDVSKPTVLLFGNEHSGVSETALHKADGNFVIPMQ
HHHHHHCCCEEEEEECCCCCCCHHHCCCCCCEEEEECCCCCCCHHHHHEECCCCEEEEHH
GYVQSLNISVAAAISLYDVTTRSRHIVTDGYELPVEDQARIFKDWMVKSLPKRTLGLYKQ
HHHHHHCEEEEEEEEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
KGVTAFERETGNSE
CCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]