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The map label for this gene is gmuD [H]

Identifier: 229917611

GI number: 229917611

Start: 1852469

End: 1853893

Strand: Direct

Name: gmuD [H]

Synonym: EAT1b_1886

Alternate gene names: 229917611

Gene position: 1852469-1853893 (Clockwise)

Preceding gene: 229917610

Following gene: 229917612

Centisome position: 61.75

GC content: 43.93

Gene sequence:

>1425_bases
ATGACGAACCCGACACAAACACGAATGAAAGAGCAAAAGACCTATCAATTTCCGACAGACTTTTGGTGGGGAACGGCTTC
TTCTGCTACACAGATGGAAGGTGCTGCTTTTGAAGGTGGAAAAGGATTGAACATCTGGGATTACTGGTATATGCAAGAGC
CGAACCGCTTCTTCAATGGCGTGAGCGCGACAGAGGCCTCAAACTTCTATCATTTGTATGAAGAAGATATCGCATTGATG
AAAGAACTCGGGCATAACACGTTCCGTTTCTCCATTTCATGGTCACGCCTTTTTCCACAAGGTGTCGGGGAAGTAAATGA
AGAGGCAATCACATTTTATAACAATGTCATCAATTGCTTAATCGATCATGGTATCGAACCGTTCATCAATCTGTATCACT
TTGATATGCCGATGGCGCTTCAAGAACAAGGTGGCTGGGAGTCACGGGAAGTCGTGGAGGCATATGTCACCTTTGCGAAG
ACATGCTTTGAGTTGTTTGGTGACCGCGTCGAGAAGTGGTTCACGCATAATGAACCGATTGTACCGGTAGAGGGTGGATA
TTTATATGATTTTCATTATCCAAACGTTGTTGATTTCAAACGAGCTGTCCAAGTGGCGTACCACTCGATCTTATCGAGTG
CGATGGCAATCGAATCGTATCGTGACATGGGACAATCAGGGCAAATCGGAATCATCTTAAATCTGACACCCTCATATCCG
AGAAGTCAGCACCCAGCTGACTTGAGCGCAGCGCGCATTGCTGATGCGATGTTCAATCGCTCGTTCCTCGATCCATCTGT
TTTAGGCACGTTCCCTCAAGAATTAGTAGATGTGCTGAAGTCGGAAGATGCGATGCCACAGATGGAAGCAGGCGATCTTG
AACTCATTCGAGAACATACAGTCGATCTGCTTGGCATCAACTATTACCAGCCGCGTCGCGTGAAAGCGAAAGCATCGCTT
CCGAATCCGGACGCACCATTCTTACCGGAAAACTTATTTGATTTCTACGAGATGCCTGGACGTAAAATGAATCCATATCG
TGGATGGGAAATTTACGAAAAAGGAATCTATGATATTTTAATCAATCTAAAAGAAAACTATGGTAATATTCCATGCTTCA
TTTCAGAGAATGGAATGGGTGTCGAAGGTGAAGAGCGTTATCGTGACGAGAACGGAAGTGTCCAAGACGATTATCGGATT
GACTTCATTCGTGAACATCTGAAATGGATGCATCAAGCGATTGAAGAGGGTTCGAAGGTGAAAGGCTATCATCTTTGGAC
GTTCATGGACAATTGGTCATGGAGCAACGCTTATAAAAATCGCTATGGATTTGTAGGTATTGATTTAGATACGCAACAGC
GTACTGTAAAAAAATCAGGATACTGGTTTAAAGAATTGATTGAAGCGAAAGGTTTCACAGAATAA

Upstream 100 bases:

>100_bases
ATCGACCGCATGAACTTGAAAAAAGAGTTAGAAGAATCAACAGATAAACAAGAAGCAGTGTAAACAAATAGAGAAAATAG
AAATAAGGGAGAGATAAAAG

Downstream 100 bases:

>100_bases
TGAAGCGAACTGACAAAGACTACTCTTTGTCAGTTTTTTTTGATATATTGTACATATATGTCTATACATGAGGTGCTATA
TGTCTACTAAGAAAAAACCA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTNPTQTRMKEQKTYQFPTDFWWGTASSATQMEGAAFEGGKGLNIWDYWYMQEPNRFFNGVSATEASNFYHLYEEDIALM
KELGHNTFRFSISWSRLFPQGVGEVNEEAITFYNNVINCLIDHGIEPFINLYHFDMPMALQEQGGWESREVVEAYVTFAK
TCFELFGDRVEKWFTHNEPIVPVEGGYLYDFHYPNVVDFKRAVQVAYHSILSSAMAIESYRDMGQSGQIGIILNLTPSYP
RSQHPADLSAARIADAMFNRSFLDPSVLGTFPQELVDVLKSEDAMPQMEAGDLELIREHTVDLLGINYYQPRRVKAKASL
PNPDAPFLPENLFDFYEMPGRKMNPYRGWEIYEKGIYDILINLKENYGNIPCFISENGMGVEGEERYRDENGSVQDDYRI
DFIREHLKWMHQAIEEGSKVKGYHLWTFMDNWSWSNAYKNRYGFVGIDLDTQQRTVKKSGYWFKELIEAKGFTE

Sequences:

>Translated_474_residues
MTNPTQTRMKEQKTYQFPTDFWWGTASSATQMEGAAFEGGKGLNIWDYWYMQEPNRFFNGVSATEASNFYHLYEEDIALM
KELGHNTFRFSISWSRLFPQGVGEVNEEAITFYNNVINCLIDHGIEPFINLYHFDMPMALQEQGGWESREVVEAYVTFAK
TCFELFGDRVEKWFTHNEPIVPVEGGYLYDFHYPNVVDFKRAVQVAYHSILSSAMAIESYRDMGQSGQIGIILNLTPSYP
RSQHPADLSAARIADAMFNRSFLDPSVLGTFPQELVDVLKSEDAMPQMEAGDLELIREHTVDLLGINYYQPRRVKAKASL
PNPDAPFLPENLFDFYEMPGRKMNPYRGWEIYEKGIYDILINLKENYGNIPCFISENGMGVEGEERYRDENGSVQDDYRI
DFIREHLKWMHQAIEEGSKVKGYHLWTFMDNWSWSNAYKNRYGFVGIDLDTQQRTVKKSGYWFKELIEAKGFTE
>Mature_473_residues
TNPTQTRMKEQKTYQFPTDFWWGTASSATQMEGAAFEGGKGLNIWDYWYMQEPNRFFNGVSATEASNFYHLYEEDIALMK
ELGHNTFRFSISWSRLFPQGVGEVNEEAITFYNNVINCLIDHGIEPFINLYHFDMPMALQEQGGWESREVVEAYVTFAKT
CFELFGDRVEKWFTHNEPIVPVEGGYLYDFHYPNVVDFKRAVQVAYHSILSSAMAIESYRDMGQSGQIGIILNLTPSYPR
SQHPADLSAARIADAMFNRSFLDPSVLGTFPQELVDVLKSEDAMPQMEAGDLELIREHTVDLLGINYYQPRRVKAKASLP
NPDAPFLPENLFDFYEMPGRKMNPYRGWEIYEKGIYDILINLKENYGNIPCFISENGMGVEGEERYRDENGSVQDDYRID
FIREHLKWMHQAIEEGSKVKGYHLWTFMDNWSWSNAYKNRYGFVGIDLDTQQRTVKKSGYWFKELIEAKGFTE

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=481, Percent_Identity=30.7692307692308, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI32481206, Length=483, Percent_Identity=30.0207039337474, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI13273313, Length=456, Percent_Identity=28.7280701754386, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI28376633, Length=486, Percent_Identity=27.7777777777778, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI24497614, Length=486, Percent_Identity=27.3662551440329, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI190360571, Length=131, Percent_Identity=36.6412213740458, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1789070, Length=489, Percent_Identity=35.3783231083845, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI2367174, Length=497, Percent_Identity=33.6016096579477, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI2367270, Length=472, Percent_Identity=31.3559322033898, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17552856, Length=474, Percent_Identity=33.3333333333333, Blast_Score=237, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI17539390, Length=472, Percent_Identity=33.0508474576271, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI21356577, Length=466, Percent_Identity=28.1115879828326, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55066; Mature: 54935

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNPTQTRMKEQKTYQFPTDFWWGTASSATQMEGAAFEGGKGLNIWDYWYMQEPNRFFNG
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCHHHHCC
VSATEASNFYHLYEEDIALMKELGHNTFRFSISWSRLFPQGVGEVNEEAITFYNNVINCL
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHH
IDHGIEPFINLYHFDMPMALQEQGGWESREVVEAYVTFAKTCFELFGDRVEKWFTHNEPI
HHCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VPVEGGYLYDFHYPNVVDFKRAVQVAYHSILSSAMAIESYRDMGQSGQIGIILNLTPSYP
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
RSQHPADLSAARIADAMFNRSFLDPSVLGTFPQELVDVLKSEDAMPQMEAGDLELIREHT
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
VDLLGINYYQPRRVKAKASLPNPDAPFLPENLFDFYEMPGRKMNPYRGWEIYEKGIYDIL
HHEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHEE
INLKENYGNIPCFISENGMGVEGEERYRDENGSVQDDYRIDFIREHLKWMHQAIEEGSKV
EEEHHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KGYHLWTFMDNWSWSNAYKNRYGFVGIDLDTQQRTVKKSGYWFKELIEAKGFTE
CCEEEEEEECCCCCCHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TNPTQTRMKEQKTYQFPTDFWWGTASSATQMEGAAFEGGKGLNIWDYWYMQEPNRFFNG
CCCHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEEEEECCCHHHHCC
VSATEASNFYHLYEEDIALMKELGHNTFRFSISWSRLFPQGVGEVNEEAITFYNNVINCL
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHH
IDHGIEPFINLYHFDMPMALQEQGGWESREVVEAYVTFAKTCFELFGDRVEKWFTHNEPI
HHCCCHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VPVEGGYLYDFHYPNVVDFKRAVQVAYHSILSSAMAIESYRDMGQSGQIGIILNLTPSYP
EECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
RSQHPADLSAARIADAMFNRSFLDPSVLGTFPQELVDVLKSEDAMPQMEAGDLELIREHT
CCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHC
VDLLGINYYQPRRVKAKASLPNPDAPFLPENLFDFYEMPGRKMNPYRGWEIYEKGIYDIL
HHEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCHHHEE
INLKENYGNIPCFISENGMGVEGEERYRDENGSVQDDYRIDFIREHLKWMHQAIEEGSKV
EEEHHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
KGYHLWTFMDNWSWSNAYKNRYGFVGIDLDTQQRTVKKSGYWFKELIEAKGFTE
CCEEEEEEECCCCCCHHHHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]