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The map label for this gene is yhaA [H]

Identifier: 229917589

GI number: 229917589

Start: 1831176

End: 1832360

Strand: Reverse

Name: yhaA [H]

Synonym: EAT1b_1864

Alternate gene names: 229917589

Gene position: 1832360-1831176 (Counterclockwise)

Preceding gene: 229917590

Following gene: 229917583

Centisome position: 61.08

GC content: 50.46

Gene sequence:

>1185_bases
ATGCTTGTGACTGACCTCGATTTAAGATTGACGGAATTATACGAAGAAATGGTGTCCCTAAGACGCCATTTTCACCAATA
TCCTGAACTGTCTTTTCAAGAAGTCGAAACACCAAAGATGATTGCCTCGTATCTCCGTGAACTTGGGATTGAAGTACGTG
AGCACGTTGGTGGCAATGGGGTCGTTGGTCGAATCAAAGGCGGTGACGGTCCGACCATCGCATTACGTGCCGATTTTGAC
GCTCTTCCCATCCAAGATGTGAAAGATGTCCCGTATCGTTCAAAAGTGAACGGGGTCATGCATGCGTGTGGACACGATGC
CCACACGGCGACCCTTCTTGTTTTAGCGAAAGTATTGACTGAGATGAGTCTTCCAGGAGACGTCGTATTGATTCACCAGT
TCGCCGAAGAATTGGCTCCAGGTGGTGCCAAACCGATGATTGAAGACGGCTGTCTCGACAATGTGGATTATATATACGGT
GCGCACATTTGGACGCCACTTCCGTTCGGGACGATTGGGGTGAAAACAGGTCCTGTGATGGCTGCGGCCGATCGCTTTGA
ATTGACCATTACAGGAAAAGGTGGACATGGCGCGATTCCGCAACATACAGTTGATGCCCTCATGGTTGCTGTGAACGTGG
CGAATCATCTCCAACAGGTCGTCAGTCGGCGAATCGATCCGCTCGAACCTGCCGTGCTGACCATCGGTACGCTCCACTCC
GGACAAGCATTCAACGTCATTGCCGAAGAAGCCAAATTATCCGGTACCGTACGTACATTCACTCGGGAGACGCAAGAAAA
AATCATCTCCGATATGGAGCGGATCATTCGTTCCGTCTGTGAGGCGAGCGATGCTGATTATGAATTGGAGTATATAAAAG
GATATCCTGCTGTCGTGAATCATGTGACCGAAACGGAACTCGTCCGTGAGAGTGCGGTGGAGGTCGTTGGTGCAGACGGT
GTCATTGAGATGGCACCACTCATGGTCGGGGAAGATTTCGCTTATTATGTCGAGCACGTTCCTGGTAGCTTTTTCTTCAC
AGGAGCAGGAAATCCCGAGCTCTCTGCAATCTTCCCGCATCATCATCCCCGCTTCGACGTGGATGAACGAGCGATGTTGC
ACACAGCCCAAGTCTTGTTAGGGGCTTTACAACGAACGTGGATGACACACGAAAAAGAGGGCTGA

Upstream 100 bases:

>100_bases
CCCGACAGCTGTTACCTCAACCGATTCTCGATGATTTGGATTCACATGACATATGGTCCTTGCTTCAACCAAAACCAATC
CACTGACAGGAGTGTTTGCT

Downstream 100 bases:

>100_bases
TGCATCGCATCAGTCCTCTTCTGTAATTGGTTCTTTTAATTCTGAAACAAGCACTTGGTCGACACGCTTTCCGTCCATAT
CTACGACTTCAAAGCGAAGA

Product: amidohydrolase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLVTDLDLRLTELYEEMVSLRRHFHQYPELSFQEVETPKMIASYLRELGIEVREHVGGNGVVGRIKGGDGPTIALRADFD
ALPIQDVKDVPYRSKVNGVMHACGHDAHTATLLVLAKVLTEMSLPGDVVLIHQFAEELAPGGAKPMIEDGCLDNVDYIYG
AHIWTPLPFGTIGVKTGPVMAAADRFELTITGKGGHGAIPQHTVDALMVAVNVANHLQQVVSRRIDPLEPAVLTIGTLHS
GQAFNVIAEEAKLSGTVRTFTRETQEKIISDMERIIRSVCEASDADYELEYIKGYPAVVNHVTETELVRESAVEVVGADG
VIEMAPLMVGEDFAYYVEHVPGSFFFTGAGNPELSAIFPHHHPRFDVDERAMLHTAQVLLGALQRTWMTHEKEG

Sequences:

>Translated_394_residues
MLVTDLDLRLTELYEEMVSLRRHFHQYPELSFQEVETPKMIASYLRELGIEVREHVGGNGVVGRIKGGDGPTIALRADFD
ALPIQDVKDVPYRSKVNGVMHACGHDAHTATLLVLAKVLTEMSLPGDVVLIHQFAEELAPGGAKPMIEDGCLDNVDYIYG
AHIWTPLPFGTIGVKTGPVMAAADRFELTITGKGGHGAIPQHTVDALMVAVNVANHLQQVVSRRIDPLEPAVLTIGTLHS
GQAFNVIAEEAKLSGTVRTFTRETQEKIISDMERIIRSVCEASDADYELEYIKGYPAVVNHVTETELVRESAVEVVGADG
VIEMAPLMVGEDFAYYVEHVPGSFFFTGAGNPELSAIFPHHHPRFDVDERAMLHTAQVLLGALQRTWMTHEKEG
>Mature_394_residues
MLVTDLDLRLTELYEEMVSLRRHFHQYPELSFQEVETPKMIASYLRELGIEVREHVGGNGVVGRIKGGDGPTIALRADFD
ALPIQDVKDVPYRSKVNGVMHACGHDAHTATLLVLAKVLTEMSLPGDVVLIHQFAEELAPGGAKPMIEDGCLDNVDYIYG
AHIWTPLPFGTIGVKTGPVMAAADRFELTITGKGGHGAIPQHTVDALMVAVNVANHLQQVVSRRIDPLEPAVLTIGTLHS
GQAFNVIAEEAKLSGTVRTFTRETQEKIISDMERIIRSVCEASDADYELEYIKGYPAVVNHVTETELVRESAVEVVGADG
VIEMAPLMVGEDFAYYVEHVPGSFFFTGAGNPELSAIFPHHHPRFDVDERAMLHTAQVLLGALQRTWMTHEKEG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=422, Percent_Identity=27.2511848341232, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 43248; Mature: 43248

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTDLDLRLTELYEEMVSLRRHFHQYPELSFQEVETPKMIASYLRELGIEVREHVGGNG
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHCHHHHHHCCCCC
VVGRIKGGDGPTIALRADFDALPIQDVKDVPYRSKVNGVMHACGHDAHTATLLVLAKVLT
EEEEEECCCCCEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EMSLPGDVVLIHQFAEELAPGGAKPMIEDGCLDNVDYIYGAHIWTPLPFGTIGVKTGPVM
HHCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCE
AAADRFELTITGKGGHGAIPQHTVDALMVAVNVANHLQQVVSRRIDPLEPAVLTIGTLHS
EECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GQAFNVIAEEAKLSGTVRTFTRETQEKIISDMERIIRSVCEASDADYELEYIKGYPAVVN
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHH
HVTETELVRESAVEVVGADGVIEMAPLMVGEDFAYYVEHVPGSFFFTGAGNPELSAIFPH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCEEECCC
HHPRFDVDERAMLHTAQVLLGALQRTWMTHEKEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLVTDLDLRLTELYEEMVSLRRHFHQYPELSFQEVETPKMIASYLRELGIEVREHVGGNG
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHCHHHHHHCCCCC
VVGRIKGGDGPTIALRADFDALPIQDVKDVPYRSKVNGVMHACGHDAHTATLLVLAKVLT
EEEEEECCCCCEEEEEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EMSLPGDVVLIHQFAEELAPGGAKPMIEDGCLDNVDYIYGAHIWTPLPFGTIGVKTGPVM
HHCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCHHCCCCCCCCCCCCCCCCE
AAADRFELTITGKGGHGAIPQHTVDALMVAVNVANHLQQVVSRRIDPLEPAVLTIGTLHS
EECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
GQAFNVIAEEAKLSGTVRTFTRETQEKIISDMERIIRSVCEASDADYELEYIKGYPAVVN
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCCHHHHH
HVTETELVRESAVEVVGADGVIEMAPLMVGEDFAYYVEHVPGSFFFTGAGNPELSAIFPH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCEEECCC
HHPRFDVDERAMLHTAQVLLGALQRTWMTHEKEG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]