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The map label for this gene is gldA [H]

Identifier: 229917582

GI number: 229917582

Start: 1825900

End: 1827003

Strand: Reverse

Name: gldA [H]

Synonym: EAT1b_1857

Alternate gene names: 229917582

Gene position: 1827003-1825900 (Counterclockwise)

Preceding gene: 229917583

Following gene: 229917579

Centisome position: 60.9

GC content: 51.27

Gene sequence:

>1104_bases
ATGTCAGAACGCATTTTCATCAGTCCGGCCAAGTATGTGCAAGGGAAACAAACGATTGACCGCATCGGAGAGTTTGTCGC
TCACTTCGGTTCAAACGCTTTACTCATCGCGGATGACGTCGTATGGGAGTTGATCGGCGAACGGGTGACCAAATCTCTGC
AAGCGAGTCAGCTCAAAGCGACGAAAGCCGATTTTGTCGGAGAAGCTTCGAAGCATGAGATCACTCGAATCGCTAGAGAT
GCTAAGGAGAGTGACACAGCGCTTGTCATCGGTCTCGGCGGTGGGAAGACGCTCGATACAGCAAAAGCGGTCGCAGATGA
ATTGGATGCGCGCATCGTCATCGTACCGACCATCGCCTCTACGGATGCACCAACGAGTGCATTGTCGGTCATTTATACAG
AGGAAGGCAATTTCGAAAGTTACCGTTTCTATAAAAAGAATCCAGATCTCGTCCTTGTGGACACGGAATTGATTGCACAA
GCACCGCCACGCTTCTTAGCGTCAGGTATTGCCGATGCATTAGCCACGTGGGTGGAAGTACGAAGTGTCCTGGCATTCGG
AGGCAAGACGATGGCTGGCGGCGTCCCAACGCTTGCAGCGGAGGCGATTGCCGAACGGTGTGAAGAGGTTCTATTCACAT
ACGGGATTCAAGCATATGAAGCTGTCAAAGCAAAAGTCGTCACACCTGCGCTTGAAGCCGTTGTGGAAGCCAATACCCTA
TTGAGTGGACTTGGCTTTGAAAGCGGCGGCTTGGCTGCAGCCCACGCCATTCACAATGGATTCACCGCGCTTGAAGGAGA
CATCCACCATTTGACGCACGGTGAAAAAGTCGCATTCGGAACACTCGTACAACTCGCACTCGAAGGACGTCCTCAAGAGG
AGTTCGAACACTATGTCGCCTTTTATATGGCTCTCGACTTACCGGTCACACTCGAAGAGATCAAATTGAAGGATGCGTCT
CGAGACGACATCTTGAAAGTCGGGAAAGCCGCCACGGCAGATGGCGAAACGATTCATAGCGGTTTCGATGTCACAGCTGA
AGAAGTTGCAGACGCCATCATCGCTGCCGATCGCTATGCACGTCTTTATAAAGAACATCTATAA

Upstream 100 bases:

>100_bases
CCGTTCTTCATGTAATCTTCACGAACATTCTTTACAAACTGTAGTATGATGACCTTGTAAGCACTTACATCAAACCTGAT
ATGAAAGGATGGAGTTCACT

Downstream 100 bases:

>100_bases
AAAAACAACGAGAGGAAGCTCACACGGCTTCGTCTCGCTGTTTTTTGTTCAAAAAGAATGATAAGAGAATTCCGACTCCC
ATACAGCTGACCGTCAAAAG

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH; GlyDH [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSERIFISPAKYVQGKQTIDRIGEFVAHFGSNALLIADDVVWELIGERVTKSLQASQLKATKADFVGEASKHEITRIARD
AKESDTALVIGLGGGKTLDTAKAVADELDARIVIVPTIASTDAPTSALSVIYTEEGNFESYRFYKKNPDLVLVDTELIAQ
APPRFLASGIADALATWVEVRSVLAFGGKTMAGGVPTLAAEAIAERCEEVLFTYGIQAYEAVKAKVVTPALEAVVEANTL
LSGLGFESGGLAAAHAIHNGFTALEGDIHHLTHGEKVAFGTLVQLALEGRPQEEFEHYVAFYMALDLPVTLEEIKLKDAS
RDDILKVGKAATADGETIHSGFDVTAEEVADAIIAADRYARLYKEHL

Sequences:

>Translated_367_residues
MSERIFISPAKYVQGKQTIDRIGEFVAHFGSNALLIADDVVWELIGERVTKSLQASQLKATKADFVGEASKHEITRIARD
AKESDTALVIGLGGGKTLDTAKAVADELDARIVIVPTIASTDAPTSALSVIYTEEGNFESYRFYKKNPDLVLVDTELIAQ
APPRFLASGIADALATWVEVRSVLAFGGKTMAGGVPTLAAEAIAERCEEVLFTYGIQAYEAVKAKVVTPALEAVVEANTL
LSGLGFESGGLAAAHAIHNGFTALEGDIHHLTHGEKVAFGTLVQLALEGRPQEEFEHYVAFYMALDLPVTLEEIKLKDAS
RDDILKVGKAATADGETIHSGFDVTAEEVADAIIAADRYARLYKEHL
>Mature_366_residues
SERIFISPAKYVQGKQTIDRIGEFVAHFGSNALLIADDVVWELIGERVTKSLQASQLKATKADFVGEASKHEITRIARDA
KESDTALVIGLGGGKTLDTAKAVADELDARIVIVPTIASTDAPTSALSVIYTEEGNFESYRFYKKNPDLVLVDTELIAQA
PPRFLASGIADALATWVEVRSVLAFGGKTMAGGVPTLAAEAIAERCEEVLFTYGIQAYEAVKAKVVTPALEAVVEANTLL
SGLGFESGGLAAAHAIHNGFTALEGDIHHLTHGEKVAFGTLVQLALEGRPQEEFEHYVAFYMALDLPVTLEEIKLKDASR
DDILKVGKAATADGETIHSGFDVTAEEVADAIIAADRYARLYKEHL

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Is also able to use various diols as substrates, such as propan-1,2-diol, butan-

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=362, Percent_Identity=48.8950276243094, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI1786815, Length=349, Percent_Identity=33.2378223495702, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39373; Mature: 39242

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERIFISPAKYVQGKQTIDRIGEFVAHFGSNALLIADDVVWELIGERVTKSLQASQLKA
CCCCEEECHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
TKADFVGEASKHEITRIARDAKESDTALVIGLGGGKTLDTAKAVADELDARIVIVPTIAS
HHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECC
TDAPTSALSVIYTEEGNFESYRFYKKNPDLVLVDTELIAQAPPRFLASGIADALATWVEV
CCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
RSVLAFGGKTMAGGVPTLAAEAIAERCEEVLFTYGIQAYEAVKAKVVTPALEAVVEANTL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFESGGLAAAHAIHNGFTALEGDIHHLTHGEKVAFGTLVQLALEGRPQEEFEHYVA
HHHCCCCCCCHHHHHHHHCCCHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
FYMALDLPVTLEEIKLKDASRDDILKVGKAATADGETIHSGFDVTAEEVADAIIAADRYA
HHHHHCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RLYKEHL
HHHHHCC
>Mature Secondary Structure 
SERIFISPAKYVQGKQTIDRIGEFVAHFGSNALLIADDVVWELIGERVTKSLQASQLKA
CCCEEECHHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
TKADFVGEASKHEITRIARDAKESDTALVIGLGGGKTLDTAKAVADELDARIVIVPTIAS
HHHHHHCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEEEECC
TDAPTSALSVIYTEEGNFESYRFYKKNPDLVLVDTELIAQAPPRFLASGIADALATWVEV
CCCCCCEEEEEEECCCCCCCEEEEECCCCEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
RSVLAFGGKTMAGGVPTLAAEAIAERCEEVLFTYGIQAYEAVKAKVVTPALEAVVEANTL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGFESGGLAAAHAIHNGFTALEGDIHHLTHGEKVAFGTLVQLALEGRPQEEFEHYVA
HHHCCCCCCCHHHHHHHHCCCHHCCCCHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
FYMALDLPVTLEEIKLKDASRDDILKVGKAATADGETIHSGFDVTAEEVADAIIAADRYA
HHHHHCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RLYKEHL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]